Invesigation on signal transduction of erythropoietin in vascular smooth muscle cells

血管平滑肌细胞促红细胞生成素信号转导研究

• 批准号: 13671125
• 负责人: KUSANO Eiji
• 金额: $ 2.24万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671125/
• 关键词: signal transuduction; erythropoietin; hypertension; vasular smooth muscle cell; MAPK system; cAMP; endothelial progenitor cells; 透析患者; 心血管系合併症; 血管新生; VEGF; cAMP; 血管平滑筋細胞; MAPK; protein kinase A; 細胞増殖; 培養血管平滑筋細胞

We have examined mechanism of pathogenesis of the eythropoietin (EPO)-induced hypertension until now in cultured vascular smooth muscle cell and by dialysis patients. Since antiplatelet drug & induces inducible nitric oxide synthase (iNOS). this drug brings about direct decrease of blood pressure with the stimulation of the NO production, and the possibility of inducing decrease of blood pressure by the induction of the apoptosis of vasucular smooth muscle through the remodeling of blood vessel has been reported. In the present study, we would like to clarify the mechanism of pathogenesis of the EPO-induced hypertension by investigating the signal transduction system in vascular smooth muscle cell.

到目前为止，我们已经在培养的血管平滑肌细胞和透析患者中研究了促红细胞生成素(EPO)诱导高血压的发病机制。因为抗血小板药物可诱导诱导型一氧化氮合酶(INOS)。该药通过刺激NO的产生而直接降低血压，并可能通过血管重塑诱导血管平滑肌细胞凋亡而导致血压下降。本研究拟通过对血管平滑肌细胞内信号转导系统的研究，阐明EPO致高血压的发病机制。

项目成果

Ito C: "Cilostazol enhances IL-1β-induced NO production and apoptosis in rat vascular smooth muscle via PKA dependent pathway."Cell Signal. 14. 625-632 (2002)

Ito C：“西洛他唑通过 PKA 依赖性途径增强大鼠血管平滑肌中 IL-1β 诱导的 NO 产生和细胞凋亡。”Cell Signal。 14. 625-632 (2002)

Ito, C., Yamamoto, H., Furukawa, Y., Takeda, S., Watanabe, Y., Akimoto, T., Iimura, O., Ando, Y., Kusano, E., Asano, Y.: "Cyclic AMP inhibits PDGF-induced mesangium cell proliferation through negative expression of cyclin D1 and Cdk2."35th Annual Meeting

Ito, C.、Yamamoto, H.、Furukawa, Y.、Takeda, S.、Watanabe, Y.、Akimoto, T.、Iimura, O.、Ando, Y.、Kusano, E.、Asano, Y.：

Eizawa T: "Circulating endothelial progenitor cells are reduced in hemodialysis patients"Curr Med Res Opin. 19(7). 627-633 (2003)

Eizawa T：“血液透析患者的循环内皮祖细胞减少”Curr Med Res Opin。

Kusano, E, Akimoto, T., Sasaki, N., Ando, Y., Asano, Y.: "Hemodialysis and nitric oxide"J Artif Organs. 4. 23-29 (2001)

Kusano, E、Akimoto, T.、Sasaki, N.、Ando, Y.、Asano, Y.：“血液透析和一氧化氮”J Artif Organs。

Inoue M: "Ticlopidine upregulates interleukin-1β stimulated nitric oxide production in cultured rat vasucular smooth muscle cells"Exp Nephrol. (in press). (2002)

Inoue M：“噻氯匹定上调培养的大鼠血管平滑肌细胞中白细胞介素 1β 刺激的一氧化氮生成”Exp Nephrol（出版中）。