Distribution of wall shear stress of vascular anastomosis and Irnmuno-hlstologicai study of anastomotic Intimal hyperplasia

血管吻合口壁剪应力分布及吻合口内膜增生的免疫组织学研究

• 批准号: 13671264
• 负责人: ISHIBASHI Hiroyuki
• 金额: $ 0.7万
• 依托单位: Aichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671264/
• 关键词: Vascular anastomosis; End-to-side anastomosis; Shear stress; Anastomotic Intimal hyperplasia; Arterial re constructive surgery of leg; Late graft occlusion; Remodeling of vascular wall

The purpose of this research was to determine the pathophyslology of anastomotic intimal hyperplasia by measuring the local wall shear in an end-to-side anastomosis and by immunohistological staining of the anastomotic intimal hyperplasia.An end-to-side anastomosis was produced in rabbit common carotid arteries. A vascular cast of the anastomosis was made from the anastomosed arteries by infusing a plastic casting compound, from which an ultraviolet-transparent flow mold was constructed. The wall shear stress was measured at various sites around the anastomosis using the photochromic tracer technique : it was -1 to -3dyne/cm^2 the proximal floor and 16 to 2gdyne/cm^2 the distal floor. BrdU was administered 2 weeks after the end-to-side anastomosis. Longitudinal sections of the anastomosis were prepared and then stained immunohistologically to measure the rate of cell division at different positions. The rate of BrdU-positive-cells was highest (16%) at the distal floor opposite the toe of the anastomosis ; at other portions it was 4 to 10%. Axial sections of the anastomosis were then prepared and stained immunohistologically by MIB. However, in this series many of the operated rabbits died or suffered occlusion of the anastomoses, and no results were obtained due to the shortage of harvested specimens.The above results were presented at the 37^<th> European Society for Surgical Research in 2002 and at the Asian Chapter Congress of the International Union of Angiology in 2003.

本研究的目的是通过测量端侧吻合局部壁剪力和吻合内膜增生的免疫组织学染色来确定吻合内膜增生的病理生理学。对兔颈总动脉进行端侧吻合。将吻合的动脉注入塑料浇铸物，制成吻合口的血管浇铸物，并以此为基础构建紫外透明流模。使用光致变色示踪技术测量吻合口周围不同部位的壁剪应力：近端为-1至-3dyne/cm^2，远端为16至2gdyne/cm^2。端侧吻合2周后给予BrdU。取吻合口纵切面，免疫组织染色，测定不同部位细胞分裂率。brdu阳性细胞率在吻合趾对面远端底处最高（16%）；在其他部分是4%到10%。然后制备吻合口的轴向切片，并用MIB进行免疫组织染色。然而，在本系列手术中，许多手术兔死亡或吻合口闭塞，由于采集标本不足，未取得任何结果。上述结果发表于2002年欧洲外科研究学会和2003年国际血管学联合会亚洲分会大会。

项目成果

H.Ishibashi, Y.Kiyota, H.Ikeda, M.Ojiha: "Topical wall shear stress and intimal hyperplasia at end-to-side anastomosis"Proceedings of the 37^<th> Congress of the European Society for Surgical Research. 35-368 (2002)

H.Ishibashi、Y.Kiyota、H.Ikeda、M.Ojiha：“端侧吻合术中的局部壁剪切应力和内膜增生”欧洲外科研究学会第 37 届大会论文集。

Hiroyuki Ishibashi, Y.Kiyota, H.Ikeda, M.Ojha: "Topical wall shear stress and intimal hyperplasia at end-to-side anastomosis"Proceedings of the 37^<th> Congress of the Eur. Soc. Surg. Res.. 365-368 (2002)

Hiroyuki Ishibashi、Y.Kiyota、H.Ikeda、M.Ojha：“端侧吻合术中的局部壁剪切应力和内膜增生”第 37 届欧洲议会会议记录。

Z.S.Jackson, H.Ishibashi, A.I.Gotlieb, B.L.Langille: "Effects of anastomotic angle on vascular tissue responses at end-to-side arterial grafts"J. Vasc. Surg.. 34. 300-307 (2001)

Z.S.Jackson、H.Ishibashi、A.I.Gotlieb、B.L.Langille：“吻合角对端侧动脉移植物血管组织反应的影响”J。