Analysis of Rejection after Fetal Cardiac Cell Transplantation in Comparison with Organ Transplantation

胎儿心肌细胞移植后排斥反应与器官移植的比较分析

• 批准号: 13671382
• 负责人: NISHIMURA Kazunobu
• 金额: $ 2.11万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671382/
• 关键词: Cardiac Cell Transplantation; Rejection; Cardiac Organ Transplantation; Fetal Cardiac Cell; Echocardiography; 核医学診断

1) Study 1Cultured neonatal cardiomyocytes (6x10^6) from LEW (cell isograft group: CIG) and ACI (cell allograft group: CAG) rats were transplanted into LEW hearts. LEW to ACI heterotopic organ allografts (OAG) were compared with CIA and CAG. A total of 9 pathological parameters associated with rejection were semi-quantitatively graded in conventional histology at day 14. Large numbers of donor myocardial cells were present from day 2 through day 28 in all animals of the CIG and the CAG. The grades of leukocyte infiltration and myocardial necrosis were significantly reduced in the CAG compared with in the OAG (P<0.01). Minimal vascular pathology was observed in the vessels in the CAG, while severe vascular pathology was established in the OAG2) Study 2Four weeks after induction of myocardial infarction in rats, cultured neonatal cardiomyocyte (6x10^6) from ACI (n=8: CAG) and LEW (n=7: CIG) rats labeled with PKH fluorescent dye were transplanted into LEW hearts. Seven rats had medium injection only (Control group: COG). No immunosuppression was given in any groups. In COG, percent area change (% AC) and percent circumferential shortening (%CS) were unchanged over 8 weeks. In CIG, % AC and %CS were improved over the first 4 weeks and continued to be increased in the second 4 weeks. In CAG, %AC and %CS were improved over the first 4 weeks, while no subsequent improvement was observed. Large number of PKH positive cells, also positively stained with anti-myoglobin antibody was present at 56 day in CAG as well as CIGConclusionCell allograft transplantation revealed less rejection than organ transplantation. .Left ventricular functions were significantly improved after allogeneic cardiomyocyte transplantation as well as isogeneic cardiomyocyte transplantation. However, allogeneic cardiomyocyte transplantation was transient and declined again over time

1)研究1将来自LEW（细胞同种移植组：CIG）和ACI（细胞同种异体移植组：CAG）大鼠的培养的新生心肌细胞（6 × 10^6）移植到LEW心脏中。将LEW至ACI异位器官移植（OAG）与CIA和CAG进行比较。在第14天，在常规组织学中对与排斥反应相关的总共9个病理学参数进行半定量分级。从第2天到第28天，CIG和CAG的所有动物中均存在大量供体心肌细胞。CAG组白细胞浸润和心肌坏死程度较OAG组明显减轻（P<0.01）。在CAG中的血管中观察到最小的血管病理学，而在OAG 2）研究中建立了严重的血管病理学。在诱导大鼠心肌梗死后4周，将用PKH荧光染料标记的来自ACI（n=8：CAG）和LEW（n=7：CIG）大鼠的培养的新生心肌细胞（6 × 10^6）移植到LEW心脏中。7只大鼠仅注射培养基（对照组：COG）。任何组均未给予免疫抑制。在COG中，面积变化百分比（% AC）和周向缩短百分比（%CS）在8周内保持不变。在CIG中，% AC和%CS在前4周内有所改善，并在后4周内继续增加。在CAG中，%AC和%CS在前4周内有所改善，而随后未观察到改善。CAG和CIG术后第56天出现大量PKH阳性细胞，抗肌红蛋白抗体染色阳性。结论同种异体细胞移植的排斥反应较器官移植轻。异基因心肌细胞移植和同基因心肌细胞移植后左心功能均得到明显改善。然而，同种异体心肌细胞移植是短暂的，并随着时间的推移再次下降