Inhibitory effect of Sialyl Lewis-X oligosaccharide on reperfusion injury -Optimal timing and temperature during cardioplegia-

唾液酸路易斯-X寡糖对再灌注损伤的抑制作用-心脏停搏时的最佳时机和温度-

• 批准号: 13671416
• 负责人: HAYASHIDA Nobuhiko
• 金额: $ 2.11万
• 依托单位: KURUME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671416/
• 关键词: Sialyl Lewis-X; ischemia reperfusion injury; myocardial protection; endothelial function; myocardial metabolism; 温度; 心筋保護液; ミエロペロキシターゼ活性; 顆粒球エラスターゼ

Objective: Effects of supplemental Sialyl Lewis-X analogue, a major ligand for all three selectin family members, during warm blood cardioplegia were expressed in the blood perfused isolated rat heart.Methods: The isolated hearts were arrested for 60 min with warm blood cardioplegia given at 20-min intervals. This was followed by 60 min of reperfusion. The hearts were divided into the following two groups according to the supplemental drugs added to the cardioplegic solution. The Control group (n=6) received standard warm blood cardioplegia. The SLX group (n=6) received warm blood cardioplegia supplemented with Sialyl Lewis-X analogue (60μg/ml). Cardiac function, endothelial function, myocardial metabolism and myocardial myeroperoxidase activity were assessed before and after cardioplegic arrest.Results: Left ventricular developed pressure and dp/dt were significantly (p<0.05) greater and -dp/dt was significantly (p<0.05) lower in the SLX group than the control group during reperfusion. Coronary flow at 15 min of reperfusion and NO production, when acetylcholine chloride was added were significantly (p<0.05) greater in the SLX group than the control group. Myeloperoxidase activity was significantly (p<0.05) lower in the SLX than the control group.Conclusions: The results suggest that selectin-mediated endothelial-leukocyte interactions may play an important role in myocardial ischemia and reperfusion injury. Supplementation of Sialyl Lewis-X analogue during warm blood cardioplegia may provide superior myocardial protection by suppressing leukocyte-endothelial interaction during early reperfusion period.

目的：在血液灌流的大鼠离体心上观察选择素家族3个成员的主要配体唾液酸化Lewis-X类似物在温血停搏液中的作用。然后再灌流60min。根据停搏液中添加的补充药物，将心脏分为以下两组。对照组(n=6)采用标准温血停搏液。SLX组(n=6)：温血停搏液中加入唾液酸路易斯-X类似物(60μg/ml)。结果：再灌注期SLX组左室发展压和dp/dt显著高于对照组(p<0.05)，-dp/dt显著低于对照组(p&lt；0.05)。加入氯化乙酰胆碱后，SLX组再灌流15min的冠脉流量和NO生成量均显著高于对照组(P<0.05)。SLX组髓过氧化物酶活性显著低于对照组(p&lt；0.05)。结论：选择素介导的内皮-白细胞相互作用在心肌缺血再灌注损伤中可能起重要作用。在温血停搏液中补充唾液酸路易斯-X类似物可能通过抑制再灌注早期白细胞与内皮细胞的相互作用来提供更好的心肌保护。

项目成果

N.Hayashida, et l.: "Does Off-pump Coronary Artery Bypass Grafting Really Preserve Renal Function?"Circulation Journal. 66. 921-925 (2002)

N.Hayashida 等人：“非体外循环冠状动脉旁路移植真的能保护肾功能吗？”循环杂志。

H.Teshima, et al.: "Obstruction of St.Jude Medical valves in the aortic position : Histology and immunohistrochemistry of pannus"J Thorac Cardiovase Surg. (in press).

H.Teshima 等人：“主动脉位置 St.Jude 医疗瓣膜阻塞：血管翳的组织学和免疫组织化学”J Thorac Cardiovase Surg。

N.Hayashida: "Does Off-pump Coronary Artery Bypass Grafting Really Preserve Renal Function?"Circulation Journal. 66. 921-925 (2002)

N.Hayashida：“非体外循环冠状动脉旁路移植真的能保留肾功能吗？”循环杂志。

H. Teshima, et al: "Obstruction of St. Jude Medical valves in the aortic position: History and immunohistrochemistry of pannus"J Thorac Surg. in press.

H. Teshima 等人：“主动脉位置 St. Jude 医疗瓣膜阻塞：血管翳的历史和免疫组织化学”J Thorac Surg。

H.Teshima, et al.: "Detection of Pannus by Multidetector-Row Computed Tomography"Ann Thorac Surg. 75. 1631-1633 (2003)

H.Teshima 等人：“通过多探测器行计算机断层扫描检测血管翳”Ann Thorac Surg。