Inhibitory effect of Sialyl Lewis-X oligosaccharide on reperfusion injury -Optimal timing and temperature during cardioplegia-

唾液酸路易斯-X寡糖对再灌注损伤的抑制作用-心脏停搏时的最佳时机和温度-

• 批准号: 13671416
• 负责人: HAYASHIDA Nobuhiko
• 金额: $ 2.11万
• 依托单位: KURUME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671416/
• 关键词: Sialyl Lewis-X; ischemia reperfusion injury; myocardial protection; endothelial function; myocardial metabolism; 温度; 心筋保護液; ミエロペロキシターゼ活性; 顆粒球エラスターゼ

Objective: Effects of supplemental Sialyl Lewis-X analogue, a major ligand for all three selectin family members, during warm blood cardioplegia were expressed in the blood perfused isolated rat heart.Methods: The isolated hearts were arrested for 60 min with warm blood cardioplegia given at 20-min intervals. This was followed by 60 min of reperfusion. The hearts were divided into the following two groups according to the supplemental drugs added to the cardioplegic solution. The Control group (n=6) received standard warm blood cardioplegia. The SLX group (n=6) received warm blood cardioplegia supplemented with Sialyl Lewis-X analogue (60μg/ml). Cardiac function, endothelial function, myocardial metabolism and myocardial myeroperoxidase activity were assessed before and after cardioplegic arrest.Results: Left ventricular developed pressure and dp/dt were significantly (p<0.05) greater and -dp/dt was significantly (p<0.05) lower in the SLX group than the control group during reperfusion. Coronary flow at 15 min of reperfusion and NO production, when acetylcholine chloride was added were significantly (p<0.05) greater in the SLX group than the control group. Myeloperoxidase activity was significantly (p<0.05) lower in the SLX than the control group.Conclusions: The results suggest that selectin-mediated endothelial-leukocyte interactions may play an important role in myocardial ischemia and reperfusion injury. Supplementation of Sialyl Lewis-X analogue during warm blood cardioplegia may provide superior myocardial protection by suppressing leukocyte-endothelial interaction during early reperfusion period.

目的：补充Sialyl Lewis-X类似物的影响，一个主要的配体为所有三个选择素家族成员，在温血心脏停搏液中表达的血液灌注离体大鼠heart.Methods：离体心脏停搏60分钟，给予温血心脏停搏液在20分钟的时间间隔。然后再灌注60分钟。根据心脏停搏液中添加的补充药物将心脏分为以下两组。对照组（n=6）给予标准温血停搏液。SLX组（n=6）给予含Sialyl Lewis-X类似物（60μg/ml）的温血停搏液。结果：再灌注期间，与对照组相比，SLX组左室发展压和dp/dt明显升高（p<0.05），-dp/dt明显降低（p<0.05）。SLX组再灌注15分钟时的冠脉流量和NO生成量均显著高于对照组（p<0.05）。SLX组髓过氧化物酶活性显著低于对照组（P<0.05）。结论：选择素介导的内皮细胞-白细胞相互作用在心肌缺血再灌注损伤中可能起重要作用。在温血停搏液中加入唾液酸化Lewis-X类似物可能通过抑制再灌注早期白细胞-内皮细胞相互作用而提供上级心肌保护。

项目成果

N.Hayashida, et l.: "Does Off-pump Coronary Artery Bypass Grafting Really Preserve Renal Function?"Circulation Journal. 66. 921-925 (2002)

N.Hayashida 等人：“非体外循环冠状动脉旁路移植真的能保护肾功能吗？”循环杂志。

H.Teshima, et al.: "Obstruction of St.Jude Medical valves in the aortic position : Histology and immunohistrochemistry of pannus"J Thorac Cardiovase Surg. (in press).

H.Teshima 等人：“主动脉位置 St.Jude 医疗瓣膜阻塞：血管翳的组织学和免疫组织化学”J Thorac Cardiovase Surg。

N.Hayashida: "Does Off-pump Coronary Artery Bypass Grafting Really Preserve Renal Function?"Circulation Journal. 66. 921-925 (2002)

N.Hayashida：“非体外循环冠状动脉旁路移植真的能保留肾功能吗？”循环杂志。

H. Teshima, et al: "Obstruction of St. Jude Medical valves in the aortic position: History and immunohistrochemistry of pannus"J Thorac Surg. in press.

H. Teshima 等人：“主动脉位置 St. Jude 医疗瓣膜阻塞：血管翳的历史和免疫组织化学”J Thorac Surg。

H.Teshima, et al.: "Detection of Pannus by Multidetector-Row Computed Tomography"Ann Thorac Surg. 75. 1631-1633 (2003)

H.Teshima 等人：“通过多探测器行计算机断层扫描检测血管翳”Ann Thorac Surg。