Inhibitory effects of Sialyl Lewis^x oligosaccharide on reperfusion injury by avoidance of apoptosis
Sialyl Lewis^x 寡糖通过避免细胞凋亡对再灌注损伤的抑制作用
基本信息
- 批准号:15591505
- 负责人:
- 金额:$ 1.73万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Object : Effects of supplemental Sialyl Lewis^x analogue, a major ligand for all three selectin family members, during warm blood cardioplegia were assessed in the blood perfused isolated rat heart. We evaluated the preservation of myocardial and endothelial function, and the avoidance of apoptosis.Method : The blood-perfused isolated rat heart model was prepared with 20 Wistar rats. The isolated hearts were arrested for 60 min with warm blood cardioplegia given at 20-min intervals. This was followed by 60 min of reperfusion. The hearts were divided into the following two groups according to the supplemental drugs added to the cardioplegic solution. The control group (n=10) received standard warm blood cardioplegia. The SLX group (n=10) received warm blood cardioplegia supplemented with Sialyl Lewis^x analogue (60 μg/ml). Cardiac function, endothelial function, myocardial metabolism and myocardial myeroperoxidase activity were assessed before and after cardioplegic arrest. Tissue morph … More ology was examined by transmission electron microscopy. Apoptosis was determined by DNA fragmentation and TUNEL.Result : Cardiac function was preserved better in the SLX group than the control group during reperfusion. C.flow and NO prod were greater in the SLX group than the control group when Ach.was added. MPO in myocardial tissue, expression of E-selectin and P-selectin in endthelium were lower in the SLX group than the control group. Transmission electron microscopy showed endothelial cell damage and appearance of apoptosis body in the control group, whereas such morphological change was less in the SLX group. DNA ladder and TUNEL-positive cell was shown in the control group. The results suggest that selectin-mediated endothelial-leukocyte interactions may play an important role in myocardial ischemia and reperfusion injury. Supplementation of Sialyl Lewis^x analogue during warm blood cardioplegia may provide superior myocardial protection by suppressing leukocyte-endothelial interaction and the avoidance of myocardial/endothelial apoptosis during early reperfusion period. Less
目的:观察三种选择素家族成员的主要配体Sialyl Lewis^x类似物在离体大鼠血灌注心脏温血停跳时的作用。我们评估了心肌和内皮功能的保存,以及细胞凋亡的避免。方法:20只Wistar大鼠制备血灌注离体大鼠心脏模型。离体心脏停搏60分钟,每隔20分钟给予温血停搏。然后再灌注60分钟。根据停搏液中添加的补充药物将心脏分为以下两组。对照组(n=10)采用标准温血停搏。SLX组(n=10)给予温血停搏液,加用Sialyl Lewis^x类似物(60 μg/ml)。测定心脏骤停前后的心功能、内皮功能、心肌代谢和心肌过氧化物酶活性。组织形态…更多形态学用透射电子显微镜检查。细胞凋亡通过DNA片段化和TUNEL检测。结果:SLX组在再灌注过程中心功能的保存优于对照组。SLX组的C.flow和NO prod均高于对照组。是补充道。SLX组心肌组织MPO、内皮组织e -选择素和p -选择素的表达均低于对照组。透射电镜显示对照组内皮细胞损伤,出现凋亡小体,而SLX组这种形态学改变较少。对照组呈DNA阶梯状,tunel阳性细胞。提示选择素介导的内皮-白细胞相互作用可能在心肌缺血再灌注损伤中起重要作用。在温血心脏骤停期间补充Sialyl Lewis^x类似物可能通过抑制白细胞-内皮相互作用和避免心肌/内皮细胞凋亡在早期再灌注期间提供更好的心肌保护。少
项目成果
期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
N.Hayashida, et al.: "Cardiac operations in cirrhotic patients"Ann Thorac Cadiovasc Surg. In press.
N.Hayashida 等人:“肝硬化患者的心脏手术”Ann Thorac Cadiovasc Surg。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Cardiac Operations in Cirrhotic Patients
肝硬化患者的心脏手术
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:N.Hayashida;et al.;k.Hayashida;k.Hayashida
- 通讯作者:k.Hayashida
P-Wave Signal-Averaged Electrocardigram for Predicting Atrial Arrhythmia After Cardiac Surgery
P 波信号平均心电图用于预测心脏手术后房性心律失常
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:H.Hayashida
- 通讯作者:H.Hayashida
N.Hayashida, et al.: "Clinical outcome after cardiac operations in patients with cirrhosis"Ann Thorac Surg. 77. 500-505 (2004)
N.Hayashida 等人:“肝硬化患者心脏手术后的临床结果”Ann Thorac Surg。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Clinical outcome after cardiac operations in patients with cirrhosis
- DOI:10.1016/j.athoracsur.2003.06.021
- 发表时间:2004-02-01
- 期刊:
- 影响因子:4.6
- 作者:Hayashida, N;Shoujima, T;Aoyagi, S
- 通讯作者:Aoyagi, S
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HAYASHIDA Nobuhiko其他文献
HAYASHIDA Nobuhiko的其他文献
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{{ truncateString('HAYASHIDA Nobuhiko', 18)}}的其他基金
Inhibitory effect of Sialyl Lewis-X oligosaccharide on reperfusion injury -Optimal timing and temperature during cardioplegia-
唾液酸路易斯-X寡糖对再灌注损伤的抑制作用-心脏停搏时的最佳时机和温度-
- 批准号:
13671416 - 财政年份:2001
- 资助金额:
$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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8363112 - 财政年份:2011
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biosynthesis regulation of sialyl lewis X in sera and its participation in immune response
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19790398 - 财政年份:2007
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Grant-in-Aid for Young Scientists (B)
Sialyl Lewis X binding lectins on natural killer cells and their functions
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16590465 - 财政年份:2004
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$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Application of sialyl-Lewis X conjugated liposome to the murine experimental autoimmune uveoretinitis
唾液酸-Lewis X缀合脂质体在小鼠实验性自身免疫性葡萄膜视网膜炎中的应用
- 批准号:
15591852 - 财政年份:2003
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$ 1.73万 - 项目类别:
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SIALYL LEWIS X IN RISK ASSESSMENT FOR PERIODONTAL DISEASE
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$ 1.73万 - 项目类别:
Inhibitory effect of Sialyl Lewis-X oligosaccharide on reperfusion injury -Optimal timing and temperature during cardioplegia-
唾液酸路易斯-X寡糖对再灌注损伤的抑制作用-心脏停搏时的最佳时机和温度-
- 批准号:
13671416 - 财政年份:2001
- 资助金额:
$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Therapeutic trial of sialyl Lewis^x analogue on airway inflammation in asthma
唾液酸化Lewis^x类似物对哮喘气道炎症的治疗试验
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12670569 - 财政年份:2000
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SIALYL LEWIS X IN RISK ASSESSMENT FOR PERIODONTAL DISEASE
SIALYL LEWIS X 参与牙周病风险评估
- 批准号:
6153665 - 财政年份:1999
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$ 1.73万 - 项目类别:
SIALYL LEWIS X IN RISK ASSESSMENT FOR PERIODONTAL DISEASE
SIALYL LEWIS X 参与牙周病风险评估
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6300869 - 财政年份:1999
- 资助金额:
$ 1.73万 - 项目类别:
SIALYL LEWIS X IN RISK ASSESSMENT FOR PERIODONTAL DISEASE
SIALYL LEWIS X 参与牙周病风险评估
- 批准号:
6481888 - 财政年份:1999
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$ 1.73万 - 项目类别: