Usage of ultrasound energy in combination with chemotherapy against brain tumors

为探讨低强度超声波对C6大鼠脑胶质瘤、U105 MG、U251 MG人脑胶质瘤细胞的作用，采用低强度超声波照射C6大鼠脑胶质瘤、U105 MG、U251 MG细胞。在不同浓度的Photofrin(25，75,150 ug/mg)存在下，单独超声作用10秒，与未处理细胞相比，未处理细胞与超声处理细胞的集落数无显著差异。C6和U105对超声和药物联合治疗的反应最大。U251胶质瘤细胞似乎对声动力效应的反应最小。扫描电子显微镜对超声辐照细胞的形态评价显示，细胞表面有轻微的破坏，微血管消失，提示低强度超声波改变了细胞膜，从而导致胶质细胞膜的改变。

Cell survival rate assay was tested in order to determine the effects of low intensity ultrasound on C6 Rat Glioma, U1O5MG, U251MG Human Glioma cells in the presence of Porfimer Sodium (Photofrin).C6 Rat Glioma, U105MG, U251MG cells were exposed to ultrasound at an intensity of 0.3 W/cm2 (200 kHz). Ultrasound exposure alone for 10 seconds resulted in no significant decrease of colonies compared to non-treated cells Significant differences were obtained between ultrasound treated and untreated cells in the presence of various concentrations of Photofrin (25, 75, 150 ug/mg). C6 and U105 had the most response to ultrasound and drug combination treatment. U251 glioma cells seemed to have the least response to sonodynamic effects. Morphological evaluation of ultrasound irradiated cells with scanning electron microscopy showed minor disruption of cell surface and disappearance of microvilli.These observations suggests that low intensity ultrasound altered the cell membrane thus resulting in change in gliorna cell membrane.