Usage of ultrasound energy in combination with chemotherapy against brain tumors

使用超声能量联合化疗治疗脑肿瘤

• 批准号: 13671477
• 负责人: TSUJITA Naotaka
• 金额: $ 2.11万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671477/
• 关键词: Ultrasound; Brain-tumor; Chemotherapy; Device; Cancer

Cell survival rate assay was tested in order to determine the effects of low intensity ultrasound on C6 Rat Glioma, U1O5MG, U251MG Human Glioma cells in the presence of Porfimer Sodium (Photofrin).C6 Rat Glioma, U105MG, U251MG cells were exposed to ultrasound at an intensity of 0.3 W/cm2 (200 kHz). Ultrasound exposure alone for 10 seconds resulted in no significant decrease of colonies compared to non-treated cells Significant differences were obtained between ultrasound treated and untreated cells in the presence of various concentrations of Photofrin (25, 75, 150 ug/mg). C6 and U105 had the most response to ultrasound and drug combination treatment. U251 glioma cells seemed to have the least response to sonodynamic effects. Morphological evaluation of ultrasound irradiated cells with scanning electron microscopy showed minor disruption of cell surface and disappearance of microvilli.These observations suggests that low intensity ultrasound altered the cell membrane thus resulting in change in gliorna cell membrane.

细胞存活率测定是为了确定在Porfimer Sodium（Photofrin）存在下低强度超声对C6大鼠胶质瘤、U105 MG、U251 MG人胶质瘤细胞的影响。将C6大鼠胶质瘤、U105 MG、U251 MG细胞暴露于强度为0.3W/cm 2（200 kHz）的超声。与未处理的细胞相比，单独超声暴露10秒未导致集落的显著减少。在各种浓度的Photofrin（25、75、150 μ g/mg）存在下，在超声处理的和未处理的细胞之间获得显著差异。C6和U105对超声和药物联合治疗的反应最大。U251胶质瘤细胞似乎对声动力效应的反应最小。用扫描电镜观察超声波照射后细胞的形态学变化，发现细胞表面有轻微的破坏，微绒毛消失，提示低强度的超声波改变了细胞膜，从而导致胶质细胞膜的改变。

项目成果

Tachibana K: "Neurosonology"Ultrasound Therapy. (in press).

Tachibana K：“神经超声学”超声治疗。

Ogawa K, Tachibana K, Uchida T, Tai T, Yamashita N, Tsujita N, Miyauchi R.: "High-resolution scanning welectron microscopic evaluation of cell membrane porosity by ultrasound"Med Electron Microsc日本臨床電子顕微鏡学会誌. 34. 249-253 (2002)

Okawa K、Tachibana K、Uchida T、Tai T、Yamashita N、Tsujita N、Miyauchi R.：“通过超声波对细胞膜孔隙度进行高分辨率扫描电子显微镜评估” Med Electron Microsc 日本临床电子显微镜杂志 34。 249 -253 (2002)

Ogawa K, Tachibana K, Uchida T, Tai T, Yamashita N, Tsujita N, Mivauchi R: "High-resolution scanning welectron microscopic evaluation of cell membrane porosity by ultrasound"Med Electron Microsc. 34. 249-253 (2002)

Okawa K、Tachibana K、Uchida T、Tai T、Yamashita N、Tsujita N、Mivauchi R：“通过超声波对细胞膜孔隙度进行高分辨率扫描电子显微镜评估”Med Electron Microsc。