Correlation between Histological Degree of Chondrosarcoma and Detection of MMps, ADAMTSs and TIPs

软骨肉瘤的组织学程度与MMps、ADAMTS和TIP检测的相关性

• 批准号: 13671546
• 负责人: SHUNZO Osaka
• 金额: $ 1.09万
• 依托单位: Nihon University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671546/
• 关键词: Chondrosarcoma; Malignancy; Immunochemstry; Prognosis; Matrx metalloproteinase; A disintegrin and metalloproteinase; Tissue inhibitor of metalloproteinases; 軟骨系腫瘍; マトリックスメタプロテアーゼ; 軟骨系良性腫瘍; Matrix Metalloproteinase

Background: Malignant degree of human chondrosarcoma can be difficult to determine using only histological findings. We therefore assessed expressions of matrix metalloproteinases (MMPs), a disintegrin and metalloproteinase (ADAM) with thrombospondin motifs (ADAMTSs) and tissue inhibitor of metal I oprotei nases (TIMPs) in chondrosarcoma and ascertained relationships to histological degree of malignancy and prognosis.Materials and Methods: In 28 chondrosarcoma patients, inimunostaining was performed using antibodies for each of the proteins of MMP-2, 3, 7, 9, 13, ADAMTS-4, 5 and TIMP-1, 2, 3.Results: Chondrosarcoma were classified into 7, 15 and 6 cases in histologically malignant grade I, 11 and 111, respectively. All target proteins were expressed in. chondrosarcoma. Positive correlations (p<0.05) existed between immunostaining scores and histological grades for all proteins except MMP 9, with strong correlations (p<0.01) for MMPs 2, 3 and 13, both ADAMTSs and all 3 TIMPs. No correlation existed between prognosis and immunostaining scores.Conclusions: These proteins could thus indicate degree of malignancy in human chondrosarcoma.

背景：仅凭组织学结果很难判断人软骨肉瘤的恶性程度。因此，我们检测了基质金属蛋白酶(MMPs)、去整合素和金属蛋白酶(ADAM)及其抑制物(TIMPs)在软骨肉瘤中的表达，并探讨了它们与软骨肉瘤恶性程度和预后的关系。材料和方法：对28例软骨肉瘤患者分别采用免疫组织化学方法检测基质金属蛋白酶-2、3、7、9、13、ADAMTS-4、ADAMTS-4和TIMP-1、2、3的表达。所有目的蛋白均在大肠杆菌中得到了表达。软骨肉瘤。除MMP9外，MMP2、MMP3、MMP13、ADAMTs和所有3种TIMP的表达与组织学分级均呈正相关(p&lt；0.05)。结论：这些蛋白可提示软骨肉瘤的恶性程度。

项目成果

大塚俊三他: "軟骨肉腫におけるMMP-2,MMP-9,TIMP-1,TIMP-2の免疫組織学的検討"第1回関東骨軟部腫瘍の基礎を語る会. 4 (2003)

Shunzo Otsuka 等：“软骨肉瘤中 MMP-2、MMP-9、TIMP-1 和 TIMP-2 的免疫组织学研究”第 1 届关东骨和软组织肿瘤基础会议 4 (2003)。

軟骨肉腫におけるMMps, TIMPs, ADAMs免疫組織学的検討:追加研究

软骨肉瘤中 MMps、TIMP 和 ADAM 的免疫组织学研究：其他研究

• 期刊: 日本癌治療学会雑誌(抄録)
• 作者: 大幸俊三、杉谷雅彦;吉田行弘
• 通讯作者: 吉田行弘

Correlation between histological degree of chondrosarcoma and detection of MMPs, ADAMTSs and T1MPs

软骨肉瘤组织学程度与MMPs、ADAMTSs、T1MPs检测的相关性

• 期刊: Anticancer Research (waiting)
• 作者: Osaka S;Yoshida Y;Sugitani Y
• 通讯作者: Sugitani Y