Tumor growth inhibition and cancer prevention by serene enriched garlic in murine renal adenocarcinoma

富含宁静的大蒜对小鼠肾腺癌的肿瘤生长抑制和癌症预防作用

• 批准号: 13671672
• 负责人: FUJIOKA Tomoaki
• 金额: $ 2.24万
• 依托单位: IWATE MEDICAL UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671672/
• 关键词: garlic; serene; renal cell carcinoma; apoptosis; anti-tumor effect; 腎細細胞; アポートシス; 抗腫瘍効果; 大蒜; 抗腫瘍作用; 腎癌; 発癌予防

Renca was inoculated in BALB/c mice subcutaneously or renalsubcapsularly. Various concentrations of garlic powder in saline were administered orally every two days until the day 21 and tumor volumes and weights examined, apoptosis induction in the treated tumor was also investigated by TUNEL method and RT-PCR as to Bax and bcl-2 mRNA expression. Furthermore, the concentrations of serene in tissues and sera in Renca bearing mice were measured by ICP-MS. Renca growth was inhibited and apoptosis was also induced in the tumors by treatment in a dose-dependent manner. Lower concentration of serene in tumor tisses and sera in tumor bearing mice could be correct by the garlic treatment. These finding may be important not only the treatment of renal cancer but also in its prevention

Renca 通过皮下或肾囊下接种于 BALB/c 小鼠。每两天口服不同浓度的大蒜粉盐水，直至第21天，检查肿瘤体积和重量，还通过TUNEL方法和RT-PCR研究治疗肿瘤中Bax和bcl-2 mRNA表达的细胞凋亡诱导。此外，通过 ICP-MS 测量了 Renca 小鼠组织和血清中 Serene 的浓度。通过以剂量依赖性方式治疗，Renca 生长受到抑制，肿瘤中细胞凋亡也被诱导。大蒜治疗可以纠正荷瘤小鼠肿瘤组织和血清中 Serene 浓度较低的问题。这些发现不仅对于肾癌的治疗而且对于肾癌的预防也很重要

项目成果

福 文彦, 岩動 一将, 他: "マウス腎癌におけるニンニクの抗腫瘍効果"Biotherapy. 15(3). 359-362 (2001)

Fukuhiko Fuku、Kazumasa Iwado 等人：“大蒜对小鼠肾癌的抗肿瘤作用”生物疗法 15(3) 359-362 (2001)。

旗福文彦, 岩動一蒋, 小原 航, 加藤利基, 鈴木 泰, 藤岡知昭: "マウス腎癌におけるニンニクの抗腫瘍効果"Biotherapy. 15・3. 359-362 (2001)

Fumihiko Hatafuku、Kazumasa Iwado、Wataru Ohara、Toshiki Kato、Yasushi Suzuki、Tomoaki Fujioka：“大蒜对小鼠肾癌的抗肿瘤作用”15・3（2001）。

Hatafuku F, Isurugi M, Obara H, Kato T and Fujioka: "Tumor growth inhibition by garlic in murine renal Adenocarcinoma"Biotherapy. 15. 359-362 (2001)

Hatafuku F、Isurugi M、Obara H、Kato T 和 Fujioka：“大蒜对小鼠肾腺癌的肿瘤生长抑制作用”生物疗法。

旗福文彦, 岩動一将, 小原 航, 加藤利基, 鈴木 泰, 藤岡知昭: "マウス賢癌におけるニンニクの坑腫瘍効果"Biotherapy. 15. 359-362 (2001)

Fumihiko Hatafuku、Kazumasa Iwado、Wataru Ohara、Toshiki Kato、Yasushi Suzuki、Tomoaki Fujioka：“大蒜对小鼠癌症的抗肿瘤作用”生物疗法。

藤岡知昭: "ニンニク、セレニュウムと癌"IMMUNOBLADDER NEWS. 6. 18-21 (2002)

Tomoaki Fujioka：“大蒜、硒和癌症”免疫膀胱新闻。6. 18-21 (2002)