STUDY OF TELOMERASE ACTIVITY AND EXPRESSION OF THE CATALYTIC SUBUNIT ASSOCIATED WITH THIS GENE IN HUMAN UROLOGICAL CANCER.

研究人类泌尿癌中端粒酶活性和与该基因相关的催化亚基的表达。

• 批准号: 09671645
• 负责人: FUJIOKA Tomoaki
• 金额: $ 1.86万
• 依托单位: IWATE MEDICAL UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF UROLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671645/
• 关键词: Telomerase activity; Telomerase-subunit; TRAP assay; RT-PCR method; renal cell carcinoma; bladder cancer; oncogene; tumor-maker; テロメラーゼ; 分子生物学; テロメラーゼ・サブセット; hEST2; hTRT; TLP1; TPI; 膀胱正常組織; 集団検診; 尿剥離細胞; TRAP Assay; 前立腺癌; テロメア長; サザンブロツ

Teromerase is the ribonucleoprotein enzyme associated with the immortalization and oncogenesis of cancer cells. Telomerase activity was examined in 47 RCCs using PCR-based telomeric repeat amplification protocol (TRAP) assay. Among 30 RCCs, 18 samples(60%) displayed the activity, white none of the normal tissues exhibited the activity. Subdivision of the tumors according to telomerase activity did not reveal any obvious difference in distribitution of tumor size, stage, histocytological subtype, or DNA-ploidy. However, statistically significant relationship between the frequency of telomerase-positive activity and serum lAP levels or tumor grade was foumd, respectively (p<0.05). These results indicate that telomerase activity may be an important factor for' evaluating the malignancy potential of RCC, rather than for detecting malignancy of the kidney.Although TRAP assay is a highly sensitivity method, it has been reported that Taq inhibitor and other factors in method might cause false results during PCR amplification. Recently, three new major components of telomerase genes which are hTR, TLP1/TPI, and hEST2/hTRT has been cloned. In 27 human urinary bladder cancers, expression of hEST2/hTRT and TLP1/TPI was studied by using revel-se transcriptase-polymerase chain reaction (RT-PCR) method. In all 27 cancers (100%), hEST2/hTRT expression was detected, while TLP1/TPL was displayed in 25 of 27 speciniens(92%). Five normal bladder tissues showed no expression of hEST2/hTRT, but 3 of 5 tisses (60%) did TLPl/TP1 expression. These results indicated that RT-PCR for detecting expression of hEST2/hTRP gene may be a powerful method for the mass screening or diagnosis of urinary bladder.

Teromerase是与癌细胞的永生化和肿瘤发生有关的核糖核蛋白酶。使用基于PCR的端粒重复扩增方案（TRAP）测定法，在47个RCC中检查了端粒酶活性。在30个RCC中，有18个样品（60％）显示活性，白色没有正常组织表现出活性。根据端粒酶活性的肿瘤细分并未揭示肿瘤大小，阶段，组织化学亚型或DNA-倍性的分布差异。然而，端粒酶阳性活性和血清圈水平或肿瘤等级之间具有统计学意义的关系（p <0.05）。这些结果表明，端粒酶活性可能是评估RCC的恶性肿潜力的重要因素，而不是检测肾脏的恶性肿瘤。尽管陷阱测定是一种高度敏感的方法，据报道，taq抑制剂和其他因素在PCR放大过程中可能会导致错误结果。最近，克隆了HTR，TLP1/TPI和HEST2/HTRT的三个新的主要组成部分。在27种人类膀胱癌中，使用Revel-SE转录酶 - 聚合酶链反应（RT-PCR）方法研究了HEST2/HTRT和TLP1/TPI的表达。在所有27种癌症（100％）中，均检测到HEST2/HTRT表达，而TLP1/TPL在27个斑点中的25个（92％）中显示。五个正常的膀胱组织没有表达HEST2/HTRT，但5个组织中的3个（60％）为TLPL/TP1表达。这些结果表明，用于检测HEST2/HTRP基因表达的RT-PCR可能是对泌尿膀胱的质量筛查或诊断的有力方法。