The research for the differentiation of olfactory neurons and the genes regulating the expression of olfactory receptors

嗅觉神经元分化及嗅觉受体表达调控基因的研究

• 批准号: 13671779
• 负责人: MURATA Junko
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671779/
• 关键词: Neural stem cell; Inner ear hair cell; Allergic rhinitis; Posterior-superiot nasal neurectomy; Nerve regeneration

We were not able to implement our research just the same as we had planned in the project title the biggining of the research. We have developed our original research plan to two different directions.In the former research, we have investigated the development of mouse inner ear sensory epithelium, and we sometimes have compared our results to the case of olfactory neurons. Musashi1 (Msi1) is an RNA-binding protein expressed in neural stem/progenitor cells, astroglial progenitor cells and astrocytes in the vertebrate central nervous system (CNS). We investigated Msi1 expression in the young adult mice inner ear by immunohistochemistry using monoclonal antibody against Msi1. Msi1 immunostaining was found in a variety of supporting cells but not in the hair cells in the sensory epithlium. We suppose that this wide expression of Msi1 in the supporting cells of young adult mice indicates that those supporting cells might have the potential to become hair cell progenitors when hair cells are injured, but some other mechanisms would strictly inhibit this ability.We also have been doing a clinical research related to our original project theme. We have carried out posterior-superior nasal neurectomy under endonasal endoscopic observation for the patients with severe allergic rhinitis. This surgical method is relatively novel one reported at first in 1998 (Kikawada et al.,). We have been tring to evaluate the effectiveness of it. We also begin to investigate the possibility of the regeneration of posterior-superior nerve after the operation and its effect to the clinical symptoms, using immunohistochemical method.

我们没能实现我们的研究，就像我们在项目标题中计划的那样，这项研究的重要性。我们将最初的研究计划发展到两个不同的方向。在以前的研究中，我们研究了小鼠内耳感觉上皮的发育，有时我们会将我们的结果与嗅觉神经元的情况进行比较。Musashi1(Msi1)是一种RNA结合蛋白，表达于脊椎动物中枢神经系统的神经干/祖细胞、星形胶质前体细胞和星形胶质细胞。我们用抗Msi1的单抗，用免疫组织化学方法研究了Msi1在幼年小鼠内耳的表达。Msi1免疫染色见于多种支持细胞，但在感觉上皮毛细胞中未见表达。我们推测，Msi1在幼年小鼠的支持细胞中的广泛表达表明，当毛细胞受到损伤时，这些支持细胞可能具有成为毛细胞前体细胞的潜力，但其他一些机制会严格抑制这一能力。我们也一直在进行与我们最初的项目主题相关的临床研究。我们在鼻内窥镜下对重度变应性鼻炎患者行鼻后上神经切断术。这种手术方法是1998年首次报道的一种相对新颖的方法(Kikawada等人)。我们一直在努力评估它的有效性。并应用免疫组织化学方法，探讨术后后上神经再生的可能性及其对临床症状的影响。