Significance of extra-cellular matrices and adhesion molecules in the pen-neural invasion of head and neck carcinomas

细胞外基质和粘附分子在头颈癌笔神经侵犯中的意义

• 批准号: 13671785
• 负责人: KURAROMI Yuichiro
• 金额: $ 2.05万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671785/
• 关键词: head and neck carcinoma; tongue squamous cell carcinoma; acenoid cystic carcinoma; laminin; collagen; extra-cellular matrix; peri-neural invasion; invasion; metastasis; 基底膜

We have investigated the significance of extra-cellular matrices, laminin and collagen, in the invasion and metastasis of squamous cell carcinomas (SCCs) of the tongue and adenoid cystic carcinomas (ACCs) of the head and neck. Results and conclusions are described as follows:1.Laminin expression in the tongue SCCs.The expression patterns of laminin γ2 chain (LNγ2) in the surgically removed specimens of the tongue SCCs were classified as two categories. When tumor cells were attached to each other and showed expansive growth, LNγ2 was expressed only in the peripheral cells of the tumor nests (peripheral expression). In contrast, when tumor cells showed infiltrative growth diminishing cell-cell adhesion, LNγ2 expression was diffusely observed in almost all of the cells (diffuse expression). The tumors cells with LNγ2 diffuse expression highly showed pen-neural invasions and vascular invasions, and closely related to the poor prognosis. It was suggested that the tumor cells showing expans … More ive growth preserved the characters of normal squamous epithelium and matrix-dependent growth capacities, and that the tumor cells showing infiltrative growth highly expressed LNγ2 and thus obtained migration and matrix-independent capacities. It seems likely that the diffuse LNγ2 expression induce metastatic potentials and malignant phenotypes of tongue SCCs.2.Laminin and collagen expression m ACCs of the head and neck.The expression of LNγ2 and type 4 collagen was examined in the surgically removed specimens of ACCs of the head and neck. The two matrices were expressed in the surrounding areas of large tumor nests as well as in the inner borders of pseudo-cysts of cribriform type ACCs. In contrast, the matrices expression was observed only in the surrounding areas of tumor nests of tubular type ACCs. Small tumor nests budding from the large cribriform tumor nests were elongated along the nerves in areas of the pen-neural invasion. The matrices expression was still observed in the inner border of the pseudocysts as well as around the invading long tumor nests. It was reported that the tumor cells located in the peripheral area of the tumor nests and inner border of the pseudo-cysts were derived from myoepithelial origin. Therefore, our results suggest that the myoepithelial cells of ACC are lined with basement membrane matrices containing laminin and collagen, and that these cells invade through the pen-neural areas by use of the basement membrane matrices. Less

我们研究了细胞外基质，层粘连蛋白和胶原在舌鳞状细胞癌(SCCs)和头颈部腺样囊性癌(ACCs)侵袭和转移中的意义。结果和结论如下：1.层粘连蛋白在舌鳞状细胞癌中的表达：层粘连蛋白γ-2链(LN-γ-2)在手术切除的舌鳞状细胞癌标本中的表达分为两类。当肿瘤细胞相互贴附并呈膨胀性生长时，LNγ2仅在瘤巢外周细胞中表达(外周表达)。相反，当肿瘤细胞呈浸润性生长，细胞间黏附减弱时，LNγ2在几乎所有细胞中都有弥漫性表达(弥漫性表达)。LNγ2弥漫性表达的肿瘤细胞表现为神经侵袭和血管侵袭，与预后不良密切相关。有人认为，显示…膨胀的肿瘤细胞较多的生长保留了正常鳞状上皮的特征和基质依赖的生长能力，而浸润性生长的肿瘤细胞高度表达LNγ2，从而获得了迁移和非基质依赖的能力。2.头颈部鳞状细胞癌中层粘连蛋白和胶原的表达。手术切除的头颈部鳞状细胞癌标本中LNγ2和4型胶原的表达。这两种基质在筛状型ACCs的大瘤巢周围和假性囊腔内缘均有表达。相反，基质仅在管状型ACCs的肿瘤巢周围区域表达。从大的筛状肿瘤巢中长出的小肿瘤巢在周围神经侵袭区沿神经被拉长。在假性囊肿的内缘及侵袭的长瘤巢周围仍可见基质的表达。据报道，位于瘤巢周边和假性囊肿内缘的肿瘤细胞来源于肌上皮细胞。因此，我们的结果表明，ACC的肌上皮细胞被含有层粘连蛋白和胶原的基底膜基质所覆盖，这些细胞通过基底膜基质侵入周围神经区。较少

项目成果

Yasumatsu R, Nakashima T, Kuratomi Y, Hirakawa N, Azuma K, Tomita K, Cataltepe S, Silverman GA, Clayman GL, Komiyama S.: "Serum squamous ell carcinoma antigen is a useful biologic marker in patients with inverted papillomas of the sinonasal tract."Cancer.

Yasumatsu R、Nakashima T、Kuratomi Y、Hirakawa N、Azuma K、Tomita K、Cataltepe S、Silverman GA、Clayman GL、Komiyama S.：“血清鳞状细胞癌抗原是鼻窦内翻性乳头状瘤患者的有用生物标志物

倉富勇一郎, 山本智矢, 熊本芳彦, 中島寅彦, 益田宗幸, 安松隆治, 小池浩次, 小宮山荘太郎: "当科における下咽頭癌の治療成績-FAR療法、根治切除,遊離空腸再建による遠隔成績と頸部郭清の適応に関する検討-"日耳鼻. 107(in press). (2004)

Yuichiro Kuratomi、Tomoya Yamamoto、Yoshihiko Kumamoto、Torahiko Nakajima、Muneyuki Masuda、Ryuji Yasumatsu、Koji Koike、Sotaro Komiyama：“我们科室下咽癌的治疗结果——FAR治疗、根治性切除和免费空肠重建的远程结果和宫颈结果“考虑部分解剖的适应症 -”Nippon Otolaryngology 107（出版中）。

倉富勇一郎: "頭頸部扁平上皮癌における基底膜蛋白ラミニンの意義と癌治療への展開"日本耳鼻咽喉科学会会報. 105・6. 721-726 (2002)

Yuichiro Kuratomi：“基底膜蛋白层粘连蛋白在头颈部鳞状细胞癌中的意义及其在癌症治疗中的发展”日本耳鼻喉科学会通报105・6（2002）。

Karatomi Y.: "Significance and therapeutic development of basement membrane laminin in squamous cell carcinoma of the head and neck"Nippon Jibiinkoka Gakkai Kaiho. 105. 721-726 (2002)

Karatomi Y.：“基底膜层粘连蛋白在头颈部鳞状细胞癌中的意义和治疗进展”Nippon Jibiinkoka Gakkai Kaiho。

Kuratomi Y, Kumamoto M, Yasumatsu R, Nakashima T, Masuda M, Kumamoto Y, Yamamoto T, Komiyama S.: "Laminin γ2 chain expression in squamous cell carcinomas of the tongue and its clinical relevance."Nippon Zibiinkoka Gakkai Kaiho. (in press). (2004)

Kuratomi Y、Kumamoto M、Yasumatsu R、Nakashima T、Masuda M、Kumamoto Y、Yamamoto T、Komiyama S.：“舌鳞状细胞癌中层粘连蛋白 γ2 链的表达及其临床相关性。”Nippon Zibiinkoka Gakkai Kaiho（见） (2004)