Significance of extra-cellular matrices and adhesion molecules in the pen-neural invasion of head and neck carcinomas

我们研究了细胞外基质、层粘连蛋白和胶原蛋白在舌鳞状细胞癌（SCCs）和头颈部腺样囊性癌（ACCs）侵袭和转移中的意义。结果和结论如下：1。舌SCCs中层粘连蛋白的表达。层粘连蛋白γ2链（LNγ2）在舌SCCs手术切除标本中的表达模式分为两类。当肿瘤细胞相互附着并呈扩张性生长时，lnγ - 2仅在肿瘤巢的外周细胞中表达（外周表达）。相反，当肿瘤细胞呈浸润性生长，细胞间黏附减弱时，lnγ - 2在几乎所有细胞中弥漫性表达（弥漫性表达）。lnγ - 2弥漫表达的肿瘤细胞高度表现为笔神经浸润和血管浸润，与预后不良密切相关。结果表明：肿瘤细胞呈扩散性生长，保留了正常鳞状上皮的特征和依赖基质的生长能力；浸润性生长的肿瘤细胞高表达lnγ - 2，具有迁移和不依赖基质的能力。扩散的lnγ - 2表达可能诱导了舌sccs的转移潜能和恶性表型。头颈部acc中层粘连蛋白和胶原蛋白的表达。在手术切除的头颈部acc标本中检测lnγ - 2和4型胶原的表达。这两种基质在大肿瘤巢的周围区域以及筛网型acc假囊肿的内边界均有表达。相比之下，仅在管型acc的肿瘤巢周围可见到基质的表达。从大筛状肿瘤巢中萌发的小肿瘤巢在笔状神经浸润区沿神经延伸。在假性囊肿的内缘和浸润的长瘤巢周围仍观察到基质的表达。据报道，位于肿瘤巢周围和假囊肿内缘的肿瘤细胞来源于肌上皮细胞。因此，我们的研究结果表明，ACC的肌上皮细胞内衬含有层粘连蛋白和胶原的基底膜基质，这些细胞利用基底膜基质侵入笔神经区。少

We have investigated the significance of extra-cellular matrices, laminin and collagen, in the invasion and metastasis of squamous cell carcinomas (SCCs) of the tongue and adenoid cystic carcinomas (ACCs) of the head and neck. Results and conclusions are described as follows:1.Laminin expression in the tongue SCCs.The expression patterns of laminin γ2 chain (LNγ2) in the surgically removed specimens of the tongue SCCs were classified as two categories. When tumor cells were attached to each other and showed expansive growth, LNγ2 was expressed only in the peripheral cells of the tumor nests (peripheral expression). In contrast, when tumor cells showed infiltrative growth diminishing cell-cell adhesion, LNγ2 expression was diffusely observed in almost all of the cells (diffuse expression). The tumors cells with LNγ2 diffuse expression highly showed pen-neural invasions and vascular invasions, and closely related to the poor prognosis. It was suggested that the tumor cells showing expans … More ive growth preserved the characters of normal squamous epithelium and matrix-dependent growth capacities, and that the tumor cells showing infiltrative growth highly expressed LNγ2 and thus obtained migration and matrix-independent capacities. It seems likely that the diffuse LNγ2 expression induce metastatic potentials and malignant phenotypes of tongue SCCs.2.Laminin and collagen expression m ACCs of the head and neck.The expression of LNγ2 and type 4 collagen was examined in the surgically removed specimens of ACCs of the head and neck. The two matrices were expressed in the surrounding areas of large tumor nests as well as in the inner borders of pseudo-cysts of cribriform type ACCs. In contrast, the matrices expression was observed only in the surrounding areas of tumor nests of tubular type ACCs. Small tumor nests budding from the large cribriform tumor nests were elongated along the nerves in areas of the pen-neural invasion. The matrices expression was still observed in the inner border of the pseudocysts as well as around the invading long tumor nests. It was reported that the tumor cells located in the peripheral area of the tumor nests and inner border of the pseudo-cysts were derived from myoepithelial origin. Therefore, our results suggest that the myoepithelial cells of ACC are lined with basement membrane matrices containing laminin and collagen, and that these cells invade through the pen-neural areas by use of the basement membrane matrices. Less