Photodynamic effect of photoproduct originated in photosensitizer on experimental tongue squamous cell carcinoma in rat

光敏剂光产物对实验性大鼠舌鳞癌的光动力作用

• 批准号: 18592217
• 负责人: OGASAWARA Toshiyuki
• 金额: $ 2.49万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592217/
• 关键词: Tongue Cancer; Photodynamic therapy; 5-Aminolevulinic Acid; Protoporphyrin-IX; Photoproduct; 光線力学的治療; 口腔癌治療; 5-aminolevulinic acid; protoporphyrinIX; ランプ; レーザー; photofrin

Purpose: 5-aminolevulinic acid (ALA)-induced protoporphyrinlX (PpIX) has been used as a photosensitizer in photodynamic therapy (PDT) for oral cancer treatment. However, the application of ALA-PDT for oral lesion is limited in relatively early malignant tumor. The Pp-IX is produced in mitochondria of cancer cell after the ALA oral-administration and is activated optimally around 635nm irradiation to induce photodestruction of the malignant lesion. Recently, it is reported that during irradiation of ALA-PDT several photoproducts are formed. Photoprotoporphyrin is the main product with peak absorption around 670nm and is efficient photosensitizer itself. It has therefore been speculated that concomitant irradiation of ALA-sensitized lesions with 635 and adding 670nm light might be more effective in harnessing the therapeutic effects arising from both Pp-IX and its photoproduct.Material and Method: Malignant lesions of rat oral cavity (tongue, gingiva, palatal mucosa) were used in this st … More udy which was induced by 4-nitroquinoline-l-oxide (4NQ0) administration about for 6 months. At 4h after oral administration of ALA (250mg/kg), the lesions were irradiated with three type of emission wavelength of diode laser. Three type were(1) 635nm CW (80mW/150J) ; (635nm)(2) 635nm CW (80mW/150J) + 670nm CW(80mW/ 150J) ; (635nm+670nm)(3) 635nm CW (80mW/150J) +Pulsed Three Wavelengths ; 659nm 664nm 669nm(42 : 7 : 1) (80mW/150J) ; (635nm+PTW).Rats were killed at 24h after laser irradiation and oral cancer samples were excised. Ki67 and apoptosis (Apoptag) were examined immunohistochemically for each samples as treatment evaluation.Conclusion and Discussion: Assessment of decrease of tumor activity and apoptosis revealed efficacy in the 635nm + PMW group compared to 635nm alone group and 635nm+670nm group. This result suggested that concomitant irradiation of ALA-sensitized cancer with 635 nm and adding photoproduct light showed enhancing the therapeutic effects. And it might be considered that 659nm, 664nm 669nm is more effective rather than 670nm.alone for tumor as photodestruction effect of photoproduct. Less

目的：5-氨基乙酰丙酸（ALA）诱导的原卟啉lX（PpIX）已被用作口腔癌光动力疗法（PDT）中的光敏剂。然而，ALA-PDT在口腔病变中的应用仅限于相对早期的恶性肿瘤。口服 ALA 后，Pp-IX 在癌细胞线粒体中产生，并在 635nm 照射附近最佳激活，诱导恶性病变的光破坏。最近，据报道，在 ALA-PDT 照射过程中，形成了多种光产物。光原卟啉是主要产品，在670nm附近有峰值吸收，本身就是高效的光敏剂。因此，推测用 635 光和添加 670nm 光同时照射 ALA 敏感病变可能更有效地利用 Pp-IX 及其光产物产生的治疗效果。 材料和方法：本研究中使用了大鼠口腔（舌头、牙龈、腭粘膜）的恶性病变。 4-硝基喹啉-1-氧化物（4NQ0）给药约6个月。口服ALA（250mg/kg）后4h，用三种发射波长的二极管激光照射病灶。三种类型为(1) 635nm CW (80mW/150J) ； (635nm)(2) 635nm 连续波 (80mW/150J) + 670nm 连续波(80mW/150J) ; (635nm+670nm)(3) 635nm CW (80mW/150J) +脉冲三波长； 659nm 664nm 669nm(42:7:1) (80mW/150J) ; (635nm+PTW)。激光照射后24小时处死大鼠并切除口腔癌样本。对每个样本进行免疫组织化学检查Ki67和细胞凋亡(Apoptag)作为治疗评估。结论和讨论：对肿瘤活性和细胞凋亡降低的评估显示，与单独635nm组和635nm+670nm组相比，635nm+PMW组的功效。该结果表明，同时用 635 nm 照射 ALA 敏感的癌症并添加光产物光可增强治疗效果。可以认为，作为光产物的光破坏作用，659nm、664nm、669nm比单独670nm更有效。较少的

项目成果

Pathological fracture of the mandible resulting from osteomyelitis successfully treated with only intermaxillary elastic guiding.

单纯颌间弹性引导成功治疗骨髓炎引起的下颌骨病理性骨折。

• 发表时间: 2008
• 期刊: Int J Oral Maxillofac Surg 11(in press)
• 作者: Nakahara;H.;et. al.;Hiroko Kitahara;Hiroko Kitahara;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Yukari Hashiba;Shinichi Nozaki;Shinichi Nozaki;能崎 晋一;能崎 晋一;Tomihiro Otani;能崎 晋一;Shinichi Nozaki;Ogasawara T
• 通讯作者: Ogasawara T

Granular cell tumor of the lower alveolar mucosa : A case report with MIB-1 and p27 immunohistochemistry and review of the literature.

下牙槽粘膜颗粒细胞瘤：MIB-1 和 p27 免疫组织化学病例报告及文献综述。

• 发表时间: 2007
• 期刊: Asian Journal of Oral and Maxillofacial Surgery (In press)
• 作者: Matsuura;H.;Tsubota K;Sano;K.;Kitagawa;Y.;Nakamura;M.;Miyauchi;K;Ogasawara;T
• 通讯作者: T

Pathological fracture of the mandible resulting from osteomyelitis successfully treated with only intemmxillary elastic guiding

单纯腋间弹力引导成功治疗骨髓炎下颌骨病理性骨折

• 发表时间: 2008
• 期刊: Int. 1 Oral Maxillofac Surg 11(in press)
• 作者: Nakahara;H.;et. al.;Hiroko Kitahara;Hiroko Kitahara;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Yukari Hashiba;Shinichi Nozaki;Shinichi Nozaki;能崎 晋一;能崎 晋一;Tomihiro Otani;能崎 晋一;Shinichi Nozaki;Ogasawara T;T. Ogasawara
• 通讯作者: T. Ogasawara

Influence of administration methods on the accumulation of ALA-induced Pp-IX in mouse tongue tumors

• DOI: 10.1111/j.1601-0825.2006.01216.x
• 发表时间: 2006-07-01
• 期刊: ORAL DISEASES
• 影响因子: 3.8
• 作者: Ogasawara, T.;Miyoshi, N.;Kinoshita, H.
• 通讯作者: Kinoshita, H.

Pathological fracture of the mandible resulting from osteomyelitis successfully treated with only intermaxillary elastic guiding

单纯颌间弹性导向成功治疗骨髓炎下颌骨病理性骨折

• 发表时间: 2008
• 期刊: Int J Oral Maxillofac Surg. 11(in press)
• 作者: Nakahara;H.;et. al.;Hiroko Kitahara;Hiroko Kitahara;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Kunio Yoshizawa;Yukari Hashiba;Shinichi Nozaki;Shinichi Nozaki;能崎 晋一;能崎 晋一;Tomihiro Otani;能崎 晋一;Shinichi Nozaki;Ogasawara T;T. Ogasawara;Ogasawara T
• 通讯作者: Ogasawara T