Experimental studies of prevention and therapy in intractable otitis media

顽固性中耳炎防治的实验研究

• 批准号: 13671793
• 负责人: OZEKI Masashi
• 金额: $ 2.05万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671793/
• 关键词: Intractable otitis media; recurrent otitis media; H. influenzae; Adenovirus; Eustachian tube

Clinical signs and otoscopyThis increased level of inflammation was sustained for an additional 7days post-IN inoculation of NTHi, after which the mean tympanic membrane inflammation score increased to value of 2+ indicating moderate inflammation with an effusion present or suspected. This level of inflammation was maintained to day 14 after NTHi inoculation when the study was terminated.Quantitation of adherent NTHiThe mean number of NTHi adherent to floor mucus at the pharyngeal portion of the ET was significantly greater 4 days after IN inoculation than at one day post inoculation. Adherence to pharyngeal floor mucus continued to increase slightly over the remaining 10 days of observation. At the mid and tympanic portions of the ET, the number of NTHi adherent to floor mucus increased more gradually, not reaching a maximum value until day 14.NTHi adherence to roof mucus was significantly less than to floor mucus at all three portions of the ET on the days indicated. The number of NTHi adherent to cells of the mucosal epithelium in any portion (pharyngeal, mid, tympanic) or region (roof, floor) of the ET was negligible at all time points of this study.NTHi adherence to middle ear mucosa was minimal until approximately 7-10 days after inoculation when there were also clinical signs of the onset of OM as determined by both otoscopy and tympanometry and delineated above. This increase in mean adherent bacteria was significantly between days : 4 and 7 ; and 7 and 10 days.Electron microscopyNTHi were found embedded in the mucus in the ET lumen and were not observed below the depth of the ciliary tips nor were they seen adherent to the surface of any epithelial cell type in any compartment of the ET

临床症状和耳镜检查在IN接种NTHi后，这种增加的炎症水平再持续7天，此后平均鼓膜炎症评分增加至2+值，表明存在或怀疑存在渗出液的中度炎症。粘附的NTHi的定量在ET的咽部粘附到地板粘液的NTHi的平均数在IN接种后4天比接种后1天显著更大。在剩余的10天观察期内，咽底粘液的粘附继续轻微增加。在ET的中部和鼓室部分，粘附于地板粘液的NTHi的数量逐渐增加，直到第14天才达到最大值。在所示日期的ET的所有三个部分，NTHi粘附于屋顶粘液显著低于地板粘液。在本研究的所有时间点，粘附于ET的任何部分（咽、中部、鼓室）或区域（顶部、底部）中的粘膜上皮细胞的NTHi的数量是可忽略的。粘附于中耳粘膜的NTHi是最小的，直到接种后约7-10天，此时还存在OM发作的临床体征，如通过耳镜检查和鼓室测压两者确定的和上文描述的。电镜观察发现NTHi包埋在ET腔的粘液中，在纤毛尖端的深度以下没有观察到，也没有观察到它们粘附在ET的任何隔室中的任何上皮细胞类型的表面上

项目成果

宮本直哉: "咽喉頭異常感と胃食道逆流症との関連性に対する検討"口咽科. 13・3. 347-351 (2001)

宫本直哉：“咽喉异常与胃食管反流病的关系研究”口咽科学13・3（2001）。

Naoya miyamoto: "Surgery on MRSA-infected otitits media"JOHNS. 19-5. 673-675 (2003)

宫本直哉：“MRSA 感染的中耳炎手术”JOHNS。

宮本直哉: "MRSA中耳炎に対する手術的治療経験"JOHNS. 17・12. 1727-1730 (2001)

Naoya Miyamoto：“MRSA 中耳炎的手术治疗经验”JOHNS 17・1730（2001）。

Takagi S, Nakamura Y, Suzuki M. et al.: "Epitope analysis of the Japanese cypress pollen major allergen, Cha o 1, recognized by a human cell clone"Nagoya Med J. 45. 121-131 (2002)

Takagi S、Nakamura Y、Suzuki M.等：“由人类细胞克隆识别的日本扁柏花粉主要过敏原 Cha o 1 的表位分析”Nagoya Med J. 45. 121-131 (2002)

Masashi ozeki: "Feline immunodeficiency virus-mediated gene therapy of middle ear mucosa cells"Auris Nasus Larynx. 29,12. 1727-1730 (2001)

Masashi ozeki：“猫免疫缺陷病毒介导的中耳粘膜细胞基因治疗”Auris Nasus Larynx。