A study regarding the mechanism in development of endolymphatic hydrops in Meniere's disease

梅尼埃病内淋巴积水发生机制的研究

• 批准号: 13671797
• 负责人: NAGANUMA Hideaki
• 金额: $ 2.18万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671797/
• 关键词: Vasopressin; V-2receptor; endolymphatic hydrops; aquaporin; AQP-2; dehydration; 脱水; メニエール病; 内耳; 平衡障害; 聴覚障害; ABR; vasopressin

Until now, the mechanism in development of endolymphatic hydrops was not elucidated. There were some reports that V-2receptor for Vasopressin and water channel aquaporin-2 (AQP-2) in inner ear membranous labyrinth participated in development of endolymphatic hydrops. It was confirmed that endolymphatic hydrops was developed morphologically in administering vasopressin to abdominal cavity in guinea pig in previous reports. It was not, however, confirmed that this animal model could reflect the diseases with endolymphatic hydrops including Meniere's disease.In Study1, it was investigated whether the hearing disturbance was occurred in the endolymphatic hydrops animal model (Wistar rat) by administering vasopressin to abdominal cavity. As the results in Study1, it was confirmed that the hearing disturbance was occurred in 71.4%.In Study2, the time course of the hearing disturbance in the endolymphatic hydrops animal model by administering vasopressin to abdominal cavity was investigated. … More As the results in Study2, it was confirmed as follows; 1) the hearing disturbance was occurred in 30-120 minutes after administering vasopressin, 2) the recovery in hearing was found in 50%, 3) the hearing disturbance was mainly in only one side (80%).In Study3, the influence of dehydration load to the hearing disturbance in the endolymphatic hydrops animal model by administering vasopressin to abdominal cavity was investigated. As the results in Study3, in the endolymphatic hydrops animal model that dehydration for 24hrs was loaded, the hearing disturbance in only one side was occurred withn only 10 minutes after administering vasopressin. It was thought that AQP-2 was composed in the cell by dehydration load, AQP-2 moved to the cell membrane by the dosage of Vasopressin, water moved to endolymphatic cavity from the blood capillary and then the endolymphatic hydrops was occurred. It was suggested that dehydration load made the cells in membranous labyrinth get ready for the endolymphatic hydrops. Less

到目前为止，内淋巴积水的发生机制尚不清楚。有报道称，内耳膜迷路中的加压素V-2受体和水通道蛋白-2(AQP-2)参与了内淋巴积水的发生。以往的报道证实，在豚鼠腹腔内注射加压素时，会出现内淋巴积水。然而，尚不能证实该动物模型能够反映包括梅尼埃病在内的内淋巴积水的疾病。在研究1中，通过向内淋巴积水动物模型(Wistar大鼠)腹腔注射血管加压素，观察了内淋巴积水动物模型是否存在听力障碍。研究一证实71.4%发生听力障碍。研究二采用加压素腹腔注射建立内淋巴积水动物模型，观察内淋巴积水动物模型听力障碍的时程。…研究2进一步证实：1)在给予加压素后30-120分钟内出现听力障碍，2)听力恢复50%，3)听力障碍以一侧为主(80%)。结果表明，在加载脱水24小时的内淋巴积水动物模型中，仅在注射加压素10分钟后出现一侧听力障碍。认为AQP-2是通过脱水负荷在细胞内合成的，AQP-2在加压素作用下进入细胞膜，水从毛细血管进入内淋巴腔，发生内淋巴积水。提示脱水负荷使膜迷路内的细胞为内淋巴积水做好了准备。较少

项目成果

Hideki Naganuma et al.: "Three-Dimensional Analysis of Morphological Aspects of The Human Saccular Macula"Ann Oto Rhinol Laryngol. 110(11). 1017-1024 (2001)

Hideki Naganuma 等人：“人类囊状黄斑形态方面的三维分析”Ann Oto Rhinol Laryngol。

Anzai N, Kawahara K, et al.: "Divelopment of renal potassium excretion capacity in the neonatal rat"JPN J Physiol. 51. 745-752 (2001)

Anzai N，Kawahara K，等：“新生大鼠肾钾排泄能力的发展”JPN J Physiol。

Hideaki Naganuma., et al.: "Three-dimensional analysis of morphological aspects of the human saccular macula"Annals of otology, Rhinology & Laryngology. 110. 1017-1024 (2001)

Hideaki Naganuma., et al.：“人类囊状黄斑形态方面的三维分析”耳科、鼻科年鉴

Hideaki Naganuma et al.: "Three-Dimensional Analysis of Morphological Aspects of The Human Saccular Macula"Ann Oto Rhinol Laryngol. 110(11). 1017-1024 (2001)

Hideaki Naganuma 等人：“人类囊状黄斑形态方面的三维分析”Ann Oto Rhinol Laryngol。

Nishikitani M, et al.: "Serosal application of Ba^<2+> induces oscillatory chloride secretion via activation of submucosal cholinergic neurones in guineapig distal colon"Acta Physiologica Scandinavica. 174. 257-264 (2002)

Nishikitani M等人：“Ba 2 的浆膜应用通过激活豚鼠远端结肠中的粘膜下胆碱能神经元诱导振荡氯化物分泌”Acta Physiologica Scandinavica。