CLATHRIN SHEETS AND CYTOSKELETAL STRCTURES ON THE PROTOPLASMIC SURAFCE OF THE VENTRAL MEMBRANES OF OSTEOCLASTS IN CULTURE

培养中破骨细胞腹膜原生质表面的网格蛋白片和细胞骨架结构

基本信息

批准号：
13671925
负责人：
AKISAKA Toshitaka
金额：
$ 1.6万
依托单位：
ASAHI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Physical cell shearing resulted in various degrees of disruption of the basolateral (upper) membranes, cytoskeletons or cell organelles, and exposed the protoplasmic surface of vental (adhesion) membranes of steoclasts that were attached to the underlying substratum such as cover slips, mica or synthetic apatite plates. Freeze-dried replicas of the ventral membranes left behind on the substratum after cell-shearing provided 3-dimensional information on the ultrastructure of the protoplasmic membrane surface of cultured osteoclasts. An extensive area of the protoplasmic surface and various amounts of cytoskeletal structures attached to the adherent vental surface of the plasma membrane were visible. In particular, the most characteristic finding of the present study is that numerous clathrin sheets displaying various sizes, shapes and curvature were revealed on the ventral membrane. The polygon substructures of the clathrin lattices appeared to be composed of hexagons with a few pentago … More ns interspersed. They were seen at the paramarginal area where they were situated at the sites of close contact with the underlying substratum. In addition, clathrin lattices were never observed on the basolateral (upper) membranes. In favorable stereo views, most of cytoskeletons were not in direct contact with the clathrin sheets. However, a few observations indicated that possible remnants of cytoskeletons attached to clathrin latticesAlthough it is generally accepted that the cytoskeletal podosomes in motile cells such as osteoclasts play a major role in cell adhesion, the present study indicates that membrane-associated clathrin may also function during the attachment to the substrate. In this regard, clathrin is thought to be required for receptor-mediated endocytosis, but whether it may also function in cell attachment is still a matter for debate. This type of clathrin-related adhesion appears to be a previously unrecognized site of cell / substrate adhesion in osteoclasts. To assess this possible function, we focused on clathrin and related cytoskeletal elements on the ventral membranes of cultured osteoclasts Less

物理细胞剪切会导致不同程度的基底外侧（上）膜，细胞骨架或细胞细胞器，并暴露了齿状膜（粘附）膜的原生质表面，它们附着在底层底物上，例如覆盖片，米奇或合成的平均层。细胞剪切后，腹侧膜的冻干复制品在底层上留下，提供了有关培养的破骨细胞培养的原生质膜表面的超微结构的三维信息。可见质质表面的广泛区域和各种量的细胞骨架结构附着在质膜的粘附腹表面上。特别是，本研究的最有特征性的发现是，在腹膜上揭示了许多甲板，表现出各种尺寸，形状和曲率。网格蛋白晶格的多边形子结构似乎由六角形组成，其中有一些五特那……更多的NS散布在散布。它们在与基础底物紧密接触的位置的临界区域中看到。另外，从未在基底外侧（上）膜上观察到网格蛋白晶格。从有利的立体声角度来看，大多数细胞骨架都没有直接与网状蛋白片接触。然而，一些观察结果表明，附着在网状蛋白晶格上的细胞骨架的可能残留物通常被认为是，诸如整骨细胞（例如骨细胞）中的细胞骨架足以在细胞粘合剂中起主要作用，目前的研究表明，在膜相关的层流上也可以在附上膜相关的层lination pernation。在这方面，被认为是受体介导的内吞作用所必需的，但是它是否也可能在细胞附着中起作用仍然是争论的问题。这种类型的网状蛋白相关粘合剂似乎是整骨细胞中细胞 /底物粘合剂的先前未识别的位点。为了评估这一可能的功能，我们专注于培养破骨细胞腹膜上的网格蛋白和相关细胞骨架元素的较少