Responses of Hypothalamic-Pituitary-Adrenal axis to cold pressor stimulation

下丘脑-垂体-肾上腺轴对冷加压刺激的反应

• 批准号: 13672032
• 负责人: SUZUKI Koji
• 金额: $ 2.24万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672032/
• 关键词: cold pressor stimulation; HPA axis; cortisol; ACTH; CRF; β-endophine; pain threshold

Objective : Functional abnormalities of Hypothalamic-Pituitary-Adrenal (HPA) axis have been known implicated in chronic pain conditions (e.g., fibromyalgia). This study evaluated the changes in plasma endogenous opioid, HPA hormones concentration and trigeminal heat pain threshold upon cold pressor (CP) stimulation. Methods : In CP trials, ten healthy males (mean age : 25.3^^+__-1.3yrs) immersed their right hands for 2 minutes in 4℃ cold water. Mock trials were also done without the cold water. Whole blood samples were collected 5 minutes before, during, and 5, 15, 30, 45, 60 minutes after CP and mock stimulation for measuring Corticotropin-Releasing-Factor (CRF), Adrenocorticotropic hormone (ACTH), β-endorphine and cortisol. Whole blood was centrifuged for plasma separation, then frozen (-20℃) for later assay. Plasma CRF, ACTH, β-endorphine and cortisol concentrations were determined using radioimmunoassay techniques. Heat pain thresholds in subjects' right V2 region were simultaneous … More ly recorded 5 minutes before and 5, 30, 60 minutes after CPS using Thermal Sensory Analyzer (Medoc, Israel). Results : Mean plasma ACTH and β-endorphine levels significantly increased 5 minutes after cessation of CP stimulation and returned to the baseline in 60 minutes (p<0.01, One-way repeated measure ANOVA). Mean plasma cortisol levels also increased 30 minutes after cessation of CP stimulation (p<0.01), while no plasma CRF change was detectable (p=0.83). Mean heat pain thresholds did not show statistically significant changes during the experimental time course (p=0.41), since 4 of 10 subjects showed no obvious change in the heat pain thresholds even with the same CP stimulation schedule. Meanwhile, no variables observed in this study showed a significant change in the mock trial. Conclusions : These results suggest that CP stimulation induces progressive increase in plasma endogenous opioid and HPA hormones levels in healthy males, but this acute plasma endogenous opioid increase does not always associate to heat pain threshold responses in V2 skin region. Less

目的：已知在慢性疼痛条件（例如纤维肌痛）中已知下丘脑 - 垂体 - 肾上腺（HPA）轴的功能异常。这项研究评估了冷压（CP）模拟时血浆内源性阿片类药物，HPA激素浓度和三叉气疼痛阈值的变化。方法：在CP试验中，十名健康男性（平均年龄：25.3 ^^+__- 1.3yrs）将其右手浸入4℃冷水中2分钟。在没有冷水的情况下，也进行了模拟试验。在CP和模拟刺激前5分，5、15、30、45、60分钟和模拟刺激以测量皮质激素释放因子（CRF），肾上腺皮质机疗法（ACTH），β-内态和皮质醇之前，收集全血样品。将全血被离心以进行血浆分离，然后冷冻（-20℃）以进行以后测定。使用放射免疫测定技术确定血浆CRF，ACTH，β-内啡肽和皮质醇浓度。受试者右V2区域中的热疼痛阈值很简单……在CPS前5分5分，使用热感觉分析仪（MEDOC，以色列）的5、30、60分钟。结果：停止CP模拟后5分钟，平均血浆ACTH和β-内啡肽水平显着增加，并在60分钟内返回基线（P <0.01，单向重复测量ANOVA）。停止CP刺激后，平均血浆皮质醇水平也增加了30分钟（P <0.01），而没有血浆CRF变化可检测到（P = 0.83）。在实验时间过程中，平均热疼痛阈值在统计学上没有显示出统计学上的显着变化（P = 0.41），因为即使有相同的CP刺激时间表，10受试者中的4个也没有显示出热痛阈值的明显变化。平均值，而在这项研究中没有观察到的变量显示模拟试验发生了重大变化。结论：这些结果表明，CP模拟会导致健康雄性血浆内源性阿片类药物和HPA骑马水平的逐步增加，但是这种急性血浆内源性阿片类药物的增加并不总是与V2皮肤区域中的热疼痛阈值反应相关。较少的

项目成果

Suzuki K, Maekawa K, Minakuchi M, Inoue E, Kuboki T, Yatani H: "Hypothalamic-Pituitary-Adrenal Axis Hormones and Painful Cold Pressor Stimulation"14^<th> Annual Meeting of the Japanese Society for TMJ. Program and Abstracts. 80 (2001)

Suzuki K、Maekawa K、Minakuchi M、Inoue E、Kuboki T、Yatani H：“下丘脑-垂体-肾上腺轴激素和疼痛冷加压刺激”日本颞下颌关节学会第 14 届年会。

Kenji Maekawa, Koji Suzuki, et al.: "Function of β2-Adrenergic Receptors in Chronic Localized Myalgia"Journal of Orofacial Pain. (in press). (2003)

Kenji Maekawa、Koji Suzuki 等人：“β2-肾上腺素受体在慢性局部肌痛中的功能”《口面部疼痛杂志》（2003 年）。

Kenji Maekawa, Takuo Kuboki, Eitoku Inoue, Mami Inoue-Minakuchi, Koji Suzuki, Hirofumi Yatani, Glenn T. Clark: "Function of β_2-Adrenergic Receptors in Chronic Localized Myalgia"Journal of Orofacial Pain. (in press).

Kenji Maekawa、Takuo Kuboki、Eitoku Inoue、Mami Inoue-Minakuchi、Koji Suzuki、Hirofumi Yatani、Glenn T. Clark：“β_2-肾上腺素受体在慢性局部肌痛中的功能”口面部疼痛杂志（正在出版）。

Suzuki K, Maekawa K, Nakazawa Y, Kuboki T, Yatani H: "Hypothalamic-Pituitary-Adrenal Axis Hormones and Painful Cold Pressor Stimulation"Abstracts and Program 104^<th> Scientific Meeting of the Japan Prosthodontic Society. 46(108) Special Issue. 175 (2002)

Suzuki K、Maekawa K、Nakazawa Y、Kuboki T、Yatani H：“下丘脑-垂体-肾上腺轴激素和疼痛冷加压刺激”摘要和日本修复协会第 104 届科学会议计划。