Study of Denture Plaque Adhesion to Denture Surfaces with Different Surface Conditions

不同表面条件的义齿牙菌斑附着力研究

• 批准号: 13672060
• 负责人: ISHIKAWA Chieko
• 金额: $ 1.02万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672060/
• 关键词: Candida; Laser Mircroscopic; Atomic Force Microscope; Denture; 義歯; 走査型レーザー顕微鏡

Study of Microorganism Adherence to Denture Surfaces Using a Laser Microscopic- Observation of Resin Adhesion Candida at Low Magnification -A confocal laser microscopic was used in a simple procedure to observe the distribution of Candida adhering denture surfaces. Water soluble eosin (0.01% density with a dyeing time of 30 seconds and a flushing time of 60 minutes) was found to be appropriate for this method.This method is superior to existing methods because it provides a clear picture of Candida distribution at low magnification. It can be used to accurately evaluate overall Candida adherence to denture surfaces.Study of Candida Adhesion to Denture Surfaces with Different Surface Conditions-Atomic Force Microscope Observation of Denture Surface Conditions -Candida adhesion to denture linings commonly used in clinical applications was observed. Lining conditions varied "400, "1, 000, 5μ.Fluorescent observation used a laser microscopic. CFU units were used to represent degrees of Candida adherence. Surface observations were made with atomic force microscope.Adherence was substantial in all cases. However, atomic force microscope examination showed greater Candida adherence on the rough surfaces than on the smooth surfaces.The Atomic force microscope provides greater clarity and precision.

激光显微镜对义齿表面微生物黏附的研究--树脂黏附假丝酵母菌的低倍观察--用激光共聚焦显微镜观察义齿表面黏附假丝酵母菌的分布。结果表明，水溶性曙红(浓度为0.01%，染色时间为30秒，冲洗时间为60分钟)适合于这种方法。这种方法优于现有的方法，因为它在低倍放大下提供了念珠菌分布的清晰图像。对不同表面条件下义齿表面念珠菌粘附性的研究--义齿表面状况的原子力显微镜观察--临床上常用的义齿衬里与念珠菌粘附性的观察。衬里条件变化“400，”1,000，5μ。使用激光显微镜进行荧光观察。CFU单位用来表示念珠菌的黏附程度。表面观察是用原子力显微镜进行的。所有病例的粘附性都很强。然而，原子力显微镜检查显示，念珠菌在粗糙表面上的附着比在光滑表面上更强。原子力显微镜提供了更高的清晰度和精度。