Study of Denture Plaque Adhesion to Denture Surfaces with Different Surface Conditions
Study of Denture Plaque Adhesion to Denture Surfaces with Different Surface Conditions
批准号:
13672060
负责人:
ISHIKAWA Chieko
金额:
$1.02万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2002
中文摘要
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英文摘要
Study of Microorganism Adherence to Denture Surfaces Using a Laser Microscopic- Observation of Resin Adhesion Candida at Low Magnification -A confocal laser microscopic was used in a simple procedure to observe the distribution of Candida adhering denture surfaces. Water soluble eosin (0.01% density with a dyeing time of 30 seconds and a flushing time of 60 minutes) was found to be appropriate for this method.This method is superior to existing methods because it provides a clear picture of Candida distribution at low magnification. It can be used to accurately evaluate overall Candida adherence to denture surfaces.Study of Candida Adhesion to Denture Surfaces with Different Surface Conditions-Atomic Force Microscope Observation of Denture Surface Conditions -Candida adhesion to denture linings commonly used in clinical applications was observed. Lining conditions varied "400, "1, 000, 5μ.Fluorescent observation used a laser microscopic. CFU units were used to represent degrees of Candida adherence. Surface observations were made with atomic force microscope.Adherence was substantial in all cases. However, atomic force microscope examination showed greater Candida adherence on the rough surfaces than on the smooth surfaces.The Atomic force microscope provides greater clarity and precision.
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