The effects of particulate metals on osteoblast-like cells in vitro

颗粒金属对体外成骨细胞样细胞的影响

• 批准号: 13672067
• 负责人: TAKEDA Shoji
• 金额: $ 1.98万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672067/
• 关键词: metal; particulate; cytotoxicity; osteoblast-like cells; 金属粉末; 細胞生存率; ALP; 骨芽様細胞; 純金属

The effects of fifteen particulate metals on cell viability of osteoblast-like cells were studied in vitro, to determine whether an adverse effect on cells could be induced by the particulate forms or soluble ions. In addition, the dose-effects of six noncorrosive particulate metals on the two osteoblast-like cells were studied by analyzing MTT and alkaline phosphatase activity. The Al, Ti, Zr, Nb, Ta, Cr, Mo, and Fe particulates depressed cell viability at higher concentrations, but their extracts yielded no effect on cells, except for Mo. On the other hand, little difference in cell viability between particulates and extracts was observed for Cu, Si, V, W, and Co, while Mn and Ni particulates yielded more adverse effects on cells than did their extracts. Moreover, the interaction with noncorrosive particulate metals led to different cytotoxic results between MG-63 and HOS cells. The cell viability of MG-63 cells was depressed only at higher concentrations, but that of HOS cells was depressed at low concentration. Al, Ti, and Zr particulates more markedly depressed cell viability compared with Nb, Ta, and Cr particulates. Al particulates at higher concentrations increased ALP activity, but other particulates did not.

在体外研究了十五种颗粒金属对成骨细胞样细胞的细胞活力的影响，以确定颗粒形式或可溶性离子是否会诱导对细胞的不利影响。此外，通过分析 MTT 和碱性磷酸酶活性，研究了六种非腐蚀性颗粒金属对两种成骨细胞样细胞的剂量效应。 Al、Ti、Zr、Nb、Ta、Cr、Mo 和 Fe 颗粒在较高浓度下会降低细胞活力，但除 Mo 外，它们的提取物对细胞没有影响。另一方面，Cu、Si、V、W 和 Co 颗粒与提取物之间的细胞活力几乎没有差异，而 Mn 和 Ni 颗粒对细胞产生的不利影响比其提取物更大。此外，与非腐蚀性颗粒金属的相互作用导致 MG-63 和 HOS 细胞之间不同的细胞毒性结果。 MG-63细胞的细胞活力仅在较高浓度下被抑制，但HOS细胞的细胞活力在低浓度下被抑制。与 Nb、Ta 和 Cr 颗粒相比，Al、Ti 和 Zr 颗粒更显着地降低细胞活力。较高浓度的铝颗粒会增加 ALP 活性，但其他颗粒则不会。