Accelerates bone maturation with the synthetic 3-benzothiepin derivative in distraction osteogenesis in rats.

在大鼠牵张成骨过程中，使用合成的 3-苯并硫杂平衍生物加速骨成熟。

• 批准号: 13672085
• 负责人: KOBAYASHI Tadaharu
• 金额: $ 2.11万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672085/
• 关键词: mandibular distraction osteogenesis; rat model of distraction osteogenesis; micro-computed tomography; accelerates bone maturation; 合成3-benzothiepin誘導体; 下顎骨骨延長; 動物実験モデル; 骨形成関連因子

Distraction osteogenesis is a method of increasing bone length. The application of distraction osteogenesis to craniofacial surgery has altered the approach and treatment of congenital and acquired craniofacial defects. This study investigated the histologic and ultrastructural changes associated with mandibular distraction osteogenesis in animal model.Male Wister rats, 12 weeks old and weighing about 400g, were purchased from Charls River Japan. The operation was performed under general anesthesia. The skin overlaying the right hemimandible was shaved and incised, and the mandible exposed. Four 1.2 x 12-mm titanium micro screws were placed anterior and posterior to the planned osteotomy site. The mandible was osteotomized between the 2nd and 3rd molars using a diamond disk, a custom-made distraction device consisting of a Jack-screw embedded in methyl methacrylate mold was applied, and the skin and soft tissue were closed in layers. After a 5-day latency period, the mandibles were dis … More tracted at a rate of 0.2 mm twice a day for 10 days and rats were sacrificed at four different time points (5,15,36,and 50 days) for histologic examination and three-dimensional micro-computed tomographic reconstructions.After completion of distraction, obvious anterior cross-bite was recognized with a few radiopaque lines noted within the distraction gap. Three weeks after distraction, increasing radiopacity was noted within the distraction gap. Five weeks after distraction, fully ossified bony regenerate was demonstrated radiographically within the distraction gap. Histologic examination of the mandibles demonstrated typical endochondral bone formation and intramembraneous bone formation after completion of distraction. Three weeks after distraction, incresed newly formed bone was noted and resulted in a near complete closure of the distraction gap. Five weeks after distraction, the distraction gap was completely ossified and increasing evidence of regenerate bone remodeling to more mature lamellar bone was noted. In conclusion, the utility of this rat model of distraction osteogenesis was demonstrated Less

牵张成骨是一种增加骨长度的方法。牵张成骨术在颅面外科手术中的应用改变了先天性和后天性颅面缺损的入路和治疗方法。本研究在动物模型上研究了下颌骨牵张成骨的组织学和超微结构变化。雄性Wister大鼠，12周龄，体重约400克，购自日本查理斯河。手术在全身麻醉下进行。右半下颌骨上的皮肤被刮平并切开，下颌骨露出来。在计划截骨部位前后放置4枚1.2 x 12毫米钛微型螺钉。下颌第二磨牙和第三磨牙之间采用金刚石盘截骨，使用定制的由嵌入甲基丙烯酸甲酯模具的千位螺钉组成的牵引装置，分层封闭皮肤和软组织。潜伏期5 d后，以每天2次0.2 mm的速度牵引下颌骨，连续10 d，分别于5、15、36、50天4个不同时间点处死大鼠，进行组织学检查和三维显微计算机断层扫描重建。牵张完成后，可见明显的前牙交叉咬合，牵张间隙内可见少许不透线。牵张后三周，牵张间隙内桡骨不透明增加。撑开后5周，在撑开间隙内x线片显示完全骨化的骨再生。完成牵张后，下颌骨的组织学检查显示典型的软骨内骨形成和膜内骨形成。撑开后三周，新形成的骨增加，导致撑开间隙几乎完全闭合。牵张后5周，牵张间隙完全骨化，越来越多的证据表明再生骨重塑为更成熟的板层骨。结果表明，该模型在牵张成骨大鼠模型中的应用价值较低

项目成果

小林正治, 泉 健次, 本間克彦, 高田佳之, 新垣 晋, 齊藤 力: "下顎骨再建にトランスポート骨延長法を応用した2例"新潟歯学会誌. 32. 295-299 (2002)

Masaharu Kobayashi、Kenji Izumi、Katsuhiko Honma、Yoshiyuki Takada、Susumu Aragaki、Chikara Saito：“运输骨延长法应用于下颌重建的两个案例”新泻齿科杂志 32. 295-299 (2002)。