Drug Interactions between Sedatives and Analgesics in Rat Cerebral Cortical Synaptoneurosomes, Phospholipids Model Membrane, and Autonomic Nervous Activity

目前，我们利用脑电图和（通过观察患者的压力和紧张程度进行心电图实时分析、通过引入行为药理学观察装置进行行为观察、使用放射性同位素的试管结合实验、激素排泄物的定量分析、以及使用质子核磁共振分析具有广泛生物活性的内源性神经活性物质靛红，进行了以下一般研究。 ・脑电图的结果表明，脑电图成分：n波当喷他佐辛和咪达唑仑同时服用时，（13-30Hz）成分增加并延长了镇静状态。 ・喷他佐辛使交感神经活动增加，而咪达唑仑使交感神经活动减少。因此，发现喷他佐辛和咪达唑仑对交感神经的作用是直接相反的作用。 ・副交感神经活动因喷他佐辛而增加，但因咪达唑仑而减少。将添加肾上腺素的局麻药溶液注入口腔粘膜后，肾上腺静脉中的肾上腺素浓度降低，但股动脉中的肾上腺素浓度升高。 ・镇静结束后2小时后，无论使用镇痛药或镇静剂，对于焦虑感均没有效果。 ・镇静后的恶心、呕吐、头晕会因使用镇痛药而加剧。 ・我们可以尚未找到合适的方法同时服用镇静剂和镇痛剂，以最大限度地减少两种药物的副作用。・我们开发并合成了噻唑香豆素衍生物：3-(4-氯甲基噻唑-2-y)-7-二甲基-氨基色烯--2-酮和3-(5-乙酰基-4-甲基噻唑-2-基)-7-二乙基氨基色烯-2酮，适用于高效液相色谱法的靛红荧光衍生物形成剂未来，我们希望将我们的研究集中在可应用于临床研究的行为观察上，并促进将脑电图和心电图实时分析实验与生化实验相结合的合作研究，以期为“为牙科领域的患者和医疗保健提供者提供“在最佳条件下的成功手术、治疗和常规手术管理，在最佳条件下提供足够的安全性”做出贡献。

Presently, we conducted the following general studies using the electroencephalogram and( electrocardiogram real time analysis through observation of the degree of stress and tension in patients, behavioral observation by introducing a behavioral pharmacological observation apparatus, test tubes binding experiments using radioisotopes, quantitative analysis of hormone excretions, with analysis of isatine which is an endogenous nerve active substance having broad biological activities using proton-NMR.・The results of the electroencephalogram revealed that the electroencephalogram component: n-wave (13-30Hz) component increased when both pentazocine and midazolam were given together and extended the sedative state.・Sympathetic nerve activities increased with pentazocine, while midazolam decreased these activities. Therefore, the effects of pentazocine and midazolam on the sympathetic nerves were found to be directly opposite actions.・Parasympathetic nerve activities increased with pentaz … More ocine, but decreased with midazolam.・With injection of an epinephrine-added local anesthetic solution to the oral mucous membrane, the epinephrine level in the adrenal veins decreased, but the epinephrine level increased in the femoral artery.・We found no effects from administration of either analgesics or sedatives in terms of anxiety two hours after sedation was completed.・Nausea, vomiting and dizziness after sedation were exacerbated by administering analgesics.・We could not find a suitable method of administering both sedatives and analgesics to minimize the side effects of both agents.・We developed and synthesized thiazole-coumarin derivatives: 3-(4-chloromethylthiazole-2-y)-7-dimethyl-aminochromen--2-one and 3-(5-acetyl-4-methylthiazole-2-yl)-7-diethylaminochromen-2one, fluorescent derivative forming agents against isatine for high performance liquid chromatography which are applicable as fluorescent derivative forming agents for HPLS when analyzing biological functions and biokinetics.In the future, we would like to concentrate our studies in behavioral observations which can be applied to clinical studies, and to promote collaborative studies combining experiments using the electroencephalogram and electrocardiogram real time analysis with biochemical experiments, in an attempt to contribute our efforts to provide "successful operations, treatments and routine operation management with adequate safety under optimal conditions for both patients and medical care providers in the field of dentistry." Less