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Suppression of function in automatic nerve and elder rat brain synaptoneurosomes for aged cerebral infarction rats with administration of sedatives and analgesics

镇静镇痛对老年脑梗死大鼠自主神经及脑突触神经小体功能的抑制

基本信息

批准号：
17592101
负责人：
KUDO Masaru
金额：
$ 2.3万
依托单位：
Health Sciences University of Hokkaido
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Presently, we studied the effect of analgesic and sedative combined therapy to estimate the ethopharmacological activity, heart rate and body temperature, using the aged rats (SHRSP or SD), and also examined the influence for the brain of aged rats with binding experiments using radioisotopes.1. breed of aged rats: Male aged (7-10week old) Wistar SD and SHRSP rats were supplied in our center for experimental animals. In this study, we used only over 40 week old rats as aged. In the period of breeding, preparation, and operation, about seventy percent of the rats were died.2. Blood pressure: Blood pressure was determined by the tail-cuff method using a noninvasive automated blood pressure apparatus (Softron BP-98A).3. Activity, heart rate, and body temperature: We obtained and used the receiver and transmitters (PDT-400HR) of the telemeter system for small animals (Vital View 4000). Activity, heart rate, and body temperature were recorded by the telemeter system.4. Injection of Midazola … More m and Pentazocine simultaneously for the aged rats: We injected simultaneously 0. 5mg/kg Midazolam (MID 0. 5) and 6 or 12mg/kg Pentazocine (PEN 6, PEN 12) in aged rats, and prolonging hypofunction of coordinated movement were recommended as the sedative and analgesic effects.5. Injection of Midazolam and Pentazocine simultaneously for the aged SHRSP rats: The lower activities were seen in the rats injected of MID 0.5 and PEN 6 simultaneously (MID O. 5+PEN 6) 15 and 30 minutes after injection. The lower activities were seen in the rats injected of MID 0.5 and PEN 12 simultaneously 15,30 and 60 minutes after injection. There were no significant differences of activities in all groups 120 minutes after injection. There were not statistical differences of heart rate and body temperature between the experimental groups and control group for all study points.6. The in vitro protocol of GABAA receptor: GABAA receptor ligand-stimulated 36C1-influx proves to be of value for the screening of sedatives and analgesics. We could not make clear GABAA receptor ligand-stimulated 36C1-influx proves. Aged rats slaughtered by anesthetic, prevent the spread of mycoplasma contagion in our center for experimental animals.We verified ethopharmacologically the effect and the side effects of interactions between analgesics and sedatives for brain infarction aged rats. Less

目前，我们利用老龄大鼠（SHRSP或SD）研究了镇痛、镇静联合治疗的药理活性、心率和体温，并利用放射性同位素结合实验研究了镇痛、镇静联合治疗对老龄大鼠脑的影响。老龄大鼠品种：本中心提供雄性老龄（7-10周龄）Wistar SD和SHRSP大鼠作为实验动物。在这项研究中，我们只使用了40周龄以上的大鼠。在繁殖、准备和操作期间，约有70%的大鼠死亡。2 .血压：采用无创自动血压仪（Softron BP-98A），采用尾袖法测定血压。活动、心率和体温：我们获得并使用了小动物遥测系统（Vital View 4000）的接收器和发射器（PDT-400HR）。活动、心率和体温由遥测系统记录。老年大鼠同时注射咪达唑拉和戊唑嗪：同时注射0。5mg/kg咪达唑仑(mid0。5)和6或12mg/kg的喷他嗪（PEN 6, PEN 12）对老年大鼠的镇静镇痛作用被推荐。老年SHRSP大鼠同时注射咪达唑仑和戊唑嗪：注射后15和30分钟同时注射mid0.5和pen6 （mido .5 + pen6）的大鼠活性较低。同时注射MID 0.5和PEN 12的大鼠在注射后15、30和60分钟活性较低。注射后120分钟，各组小鼠活动无显著差异。实验组与对照组在各研究点心率、体温差异均无统计学意义。GABAA受体体外实验方案：GABAA受体配体刺激36c1内流对镇静镇痛药物的筛选具有重要价值。我们无法明确GABAA受体配体刺激的36c1内流证明。老龄大鼠经麻醉屠宰，预防支原体传染病在本中心实验动物的传播。我们从药理学上验证了镇痛药和镇静剂相互作用对脑梗死老年大鼠的作用和副作用。少