Suppression of function in automatic nerve and elder rat brain synaptoneurosomes for aged cerebral infarction rats with administration of sedatives and analgesics

目前，我们研究了镇痛和镇静联合治疗的效果，以老年大鼠（SHRSP或SD）为实验对象，测定其药理活性、心率和体温，并采用放射性同位素结合实验研究其对老年大鼠脑的影响.老龄大鼠品种：实验动物选用7- 10周龄雄性Wistar SD和SHRSP大鼠。在本研究中，我们只使用40周龄以上的大鼠作为老年大鼠。在饲养、准备和操作过程中，约有70%的大鼠死亡.血压：使用无创自动血压仪（Softron BP-98 A）通过尾袖法测定血压。活动、心率和体温：我们获得并使用了小动物遥测系统（Vital View 4000）的接收器和发射器（PDT-400 HR）。活动、心率和体温由遥测系统记录。Midazola注射液 关于我们 m和喷他佐辛同时给药：老龄大鼠同时注射0. 5 mg/kg咪达唑仑（MID 0. 5)6或12 mg/kg喷他佐辛（PEN 6，PEN 12）对老年大鼠的镇静镇痛作用，延长协调运动功能减退的时间.老年SHRSP大鼠同时注射咪达唑仑和喷他佐辛：同时注射MID 0.5和PEN 6（MID 0.5和PEN 6）的大鼠活动降低。5+PEN 6）注射后15和30分钟。注射MID 0.5和PEN 12后15、30和60分钟，大鼠的活动降低。注射后120分钟各组活动无显著性差异。实验组与对照组在各研究点的心率、体温差异均无统计学意义. GABAA受体的体外方案：GABAA受体配体刺激的36 C1-内流被证明是用于镇静剂和镇痛剂的筛选的价值。GABAA受体配体刺激36 C1内流的证据尚不清楚。本中心以麻醉法宰杀老年大鼠，预防支原体感染的实验动物，从动物药理学角度验证了镇痛药与镇静药相互作用对脑梗死老年大鼠的作用及副作用。少

Presently, we studied the effect of analgesic and sedative combined therapy to estimate the ethopharmacological activity, heart rate and body temperature, using the aged rats (SHRSP or SD), and also examined the influence for the brain of aged rats with binding experiments using radioisotopes.1. breed of aged rats: Male aged (7-10week old) Wistar SD and SHRSP rats were supplied in our center for experimental animals. In this study, we used only over 40 week old rats as aged. In the period of breeding, preparation, and operation, about seventy percent of the rats were died.2. Blood pressure: Blood pressure was determined by the tail-cuff method using a noninvasive automated blood pressure apparatus (Softron BP-98A).3. Activity, heart rate, and body temperature: We obtained and used the receiver and transmitters (PDT-400HR) of the telemeter system for small animals (Vital View 4000). Activity, heart rate, and body temperature were recorded by the telemeter system.4. Injection of Midazola … More m and Pentazocine simultaneously for the aged rats: We injected simultaneously 0. 5mg/kg Midazolam (MID 0. 5) and 6 or 12mg/kg Pentazocine (PEN 6, PEN 12) in aged rats, and prolonging hypofunction of coordinated movement were recommended as the sedative and analgesic effects.5. Injection of Midazolam and Pentazocine simultaneously for the aged SHRSP rats: The lower activities were seen in the rats injected of MID 0.5 and PEN 6 simultaneously (MID O. 5+PEN 6) 15 and 30 minutes after injection. The lower activities were seen in the rats injected of MID 0.5 and PEN 12 simultaneously 15,30 and 60 minutes after injection. There were no significant differences of activities in all groups 120 minutes after injection. There were not statistical differences of heart rate and body temperature between the experimental groups and control group for all study points.6. The in vitro protocol of GABAA receptor: GABAA receptor ligand-stimulated 36C1-influx proves to be of value for the screening of sedatives and analgesics. We could not make clear GABAA receptor ligand-stimulated 36C1-influx proves. Aged rats slaughtered by anesthetic, prevent the spread of mycoplasma contagion in our center for experimental animals.We verified ethopharmacologically the effect and the side effects of interactions between analgesics and sedatives for brain infarction aged rats. Less