Quantity and polymorphism analysis of fMLP receptor gene in patients with prepubertal periodontitis

n -甲酰基-蛋氨酸-亮氨酸-苯丙氨酸（fMLP）肽是通过与fMLP受体结合而起作用的吞噬细胞化学引诱剂。本研究采用实时RT-PCR技术对8例青春期前牙周炎患者及8例直系亲属的献血者的fMLP受体mRNA含量进行系统分析，并对其克隆等位基因进行直接测序。对于fMLP受体，我检测到所有患者（6.7-83.7%）和4名家庭成员（17.5-84.2%）的趋化活性下降。趋化活性降低后，fMLP受体mRNA呈升高（比对照组高1.18 ~ 8.37倍）和降低（比对照组低0.05 ~ 0.08倍）两种模式。如果一个孩子有抑郁的趋化活性，那么父母中至少有一方也被发现有抑郁活性。对于fMLP受体，我在本研究样本中未检测到snp，但通过实时RT-PCR分析，青春期前牙周炎患者及其直系亲属的fMLP受体基因中可能存在一些snp位点。

N-formyl-methionyl-leucyl-phenylalanine (fMLP) peptide are phagocyte chemoattractants that act by binding to fMLP receptor. Here I systematically analyzed the amount of fMLP receptor mRNA by real time RT-PCR and direct sequence of cloned alleles from blood donors from the 8 patients with prepubertal periodontitis and 8 immediate family members. For fMLP receptor I detected depressed chemotactic activity in all present patients (6.7-83.7%) and four family members (17.5-84.2%). There were two patterns found of the increased (1.18-8.37 times more than that of the control) and decreased (0.05-0.08 times less than that) fMLP receptor mRNA in the depressed chemotactic activity. If a child has the depressed chemotactic activity, then at least one of the parents was also found of the depressed activity. For fMLP receptor I detected no SNPs in a sample of the present study, however, there could be some sites of SNPs in fMLP receptor gene of the prepubertal periodontitis patients and immediate family members by real time RT-PCR analysis.