Quantity and polymorphism analysis of fMLP receptor gene in patients with prepubertal periodontitis

青春期前牙周炎患者fMLP受体基因数量及多态性分析

• 批准号: 13672153
• 负责人: OKADA Mitsugi
• 金额: $ 0.77万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672153/
• 关键词: Prepubertal periodontitis; Leukocytes chemotaxis; fMLP receptor; Polymorphism; Human genome; 好中球; 走化性; fMLP; real time RT-PCR

N-formyl-methionyl-leucyl-phenylalanine (fMLP) peptide are phagocyte chemoattractants that act by binding to fMLP receptor. Here I systematically analyzed the amount of fMLP receptor mRNA by real time RT-PCR and direct sequence of cloned alleles from blood donors from the 8 patients with prepubertal periodontitis and 8 immediate family members. For fMLP receptor I detected depressed chemotactic activity in all present patients (6.7-83.7%) and four family members (17.5-84.2%). There were two patterns found of the increased (1.18-8.37 times more than that of the control) and decreased (0.05-0.08 times less than that) fMLP receptor mRNA in the depressed chemotactic activity. If a child has the depressed chemotactic activity, then at least one of the parents was also found of the depressed activity. For fMLP receptor I detected no SNPs in a sample of the present study, however, there could be some sites of SNPs in fMLP receptor gene of the prepubertal periodontitis patients and immediate family members by real time RT-PCR analysis.

N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸 (fMLP) 肽是吞噬细胞趋化剂，通过与 fMLP 受体结合发挥作用。在这里，我通过实时RT-PCR和直接序列分析了来自8名青春期前牙周炎患者和8名直系亲属的献血者的fMLP受体mRNA量。对于 fMLP 受体，我检测到所有患者 (6.7-83.7%) 和四名家庭成员 (17.5-84.2%) 的趋化活性降低。在趋化活性降低中，发现fMLP受体mRNA增加（比对照多1.18-8.37倍）和减少（比对照少0.05-0.08倍）两种模式。如果孩子具有抑制的趋化活性，那么至少其中一位父母也被发现具有抑制的趋化活性。对于fMLP受体，我在本研究的样本中未检测到SNP，但通过实时RT-PCR分析，青春期前牙周炎患者及其直系亲属的fMLP受体基因中可能存在某些位点的SNP。