A study on the detection of periodontal pathogens from prepubertal periodontitis patients by PCR

PCR法检测青春期前牙周炎患者牙周病原菌的研究

• 批准号: 11672050
• 负责人: OKADA Mitsugi
• 金额: $ 2.3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672050/
• 关键词: PCR; periodontal pathogens; children; prepubertal periodontitis; periodontal disease; transmission

The purpose of this study was to detect the presence of 10 putative periodontal pathogens in plaque samples from 104 children, collected from their toothbrushes using a polymerase chain reaction (PCR). The age range of all subjects was 2-12 years.1. Both A.actinomycetemcomitans and P.gingivalis were detected in the primary and mixed dentition. The prevalence of A.actinomycetemcomitans in healthy subjects was 4.8%, and those with gingivitis and periodontitis was 6.8% and 20.0% respectively, while the prevalence of P.gingivalis was 4.8% in healthy subjects, and 9.6% and 20.0% in those with gingivitis and periodontitis, respectively. Our survey, using a toothbrush, indicated that A.actinomycetemcomitans and P.gingivalis are rarely present in oral cavities of healthy children.2. C.sputigena prevalence in healthy, gingivitis, and periodontitis subjects was 48.0%, 36.5% and 25.0%, respectively, that for C.ochracea was 100%, 89.4%, and 50.0%, respectively, and that for C.gingivalis was 96.0%, 84.7%, and 75.0%, respectively. Our results showed that C.sputigena was moderately prevalent, whereas C.ochracea and C.gingivalis were commonly detected in the oral cavities of the tested children, suggesting that all of these species become established in the early years.3. The prevalence of B.forsythus in healthy subjects was 33.3%, those with gingivitis was 63.9%, and those with periodontitis was 58.3%, while the prevalence of T.denticola was 0.0% in healthy subjects, and 18.1% and 16.7% in those with gingivitis and periodontitis, respectively. The prevalence of C.rectus was 100% in all subjects. Our survey indicated that T.denticola could be more associated with periodontal diseases and B.forsythus has a moderately prevalence, while C.rectus is most commonly present in the oral cavity of children and could be established in their early years.

本研究的目的是利用聚合酶链反应（PCR）检测104名儿童的牙菌斑样本中10种假定的牙周病原体的存在。所有受试者的年龄范围为2-12岁。在初级牙列和混合牙列中均检出放线菌和牙龈假单胞菌。健康人群放线菌球菌感染率为4.8%，牙龈炎和牙周炎患者感染率分别为6.8%和20.0%；健康人群牙龈假单胞菌感染率为4.8%，牙龈炎和牙周炎患者感染率分别为9.6%和20.0%。我们的调查，使用牙刷，表明放线菌单胞菌和牙龈单胞菌很少存在于健康儿童的口腔中。健康人群、牙龈炎人群和牙周炎人群中痰原菌的患病率分别为48.0%、36.5%和25.0%，黄螺旋菌的患病率分别为100%、89.4%和50.0%，牙龈螺旋菌的患病率分别为96.0%、84.7%和75.0%。我们的研究结果表明，在接受测试的儿童的口腔中，sputigena是中等流行的，而C.ochracea和C.gingivalis是常见的，这表明所有这些物种都是在早期建立的。健康人群中连翘菌的患病率为33.3%，牙龈炎人群中患病率为63.9%，牙周炎人群中患病率为58.3%，而健康人群中牙齿乳牙菌的患病率为0.0%，牙龈炎人群中患病率为18.1%，牙周炎人群中患病率为16.7%。所有受试者中直梭菌的患病率均为100%。我们的调查显示齿状牙霉与牙周病的关系更密切，而连翘牙霉发病率中等，而直梭牙霉最常见于儿童口腔，可在儿童早期发病。

项目成果

Mitsugi Okada,Fumiko Hayashi, et al.: "PCR detection of 5 putative periodontal pathogens in dental plaque samples from children 2 to 12 years of age"Journal of Clinical Periodontology. 28・7(in press). (2001)

Mitsugi Okada、Fumiko Hayashi 等人：“2 至 12 岁儿童牙菌斑样本中 5 种推定牙周病原体的 PCR 检测”《临床牙周病学杂志》28・7（出版中）。

Mitsugi Okada, Fumiko Hayashi, Nobuo Nagasaka: "PCR detection of 5 putative periodontal pathogens in dental plaque samples from children 2 to 12 years of age."Journal of Clinical Periodontology. 28, (in press.). (2001)

Mitsugi Okada、Fumiko Hayashi、Nobuo Nagasaka：“PCR 检测 2 至 12 岁儿童牙菌斑样本中 5 种假定的牙周病原体。”临床牙周病学杂志。