Study for oral infectious control during peripheral blood stem cell transplantation

外周血干细胞移植过程中口腔感染控制研究

• 批准号: 13672186
• 负责人: TANIMOTO Ichiro
• 金额: $ 1.86万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672186/
• 关键词: PBSCT; compromised host; oral infection control; periodontal desease; bacterial detection; leukocyte

The oral infection control of the compromised patients during peripheral blood stem cell transport (PBSCT) is very important to prevent spreading the infection to other region. We should remove periodontopathic bacteria, but there is the possibility that the treatment without the consideration causes the exposure the blood vessel system to oral bacteria. The purpose of this research is the following 3 points. 1, Setting screening parameter item to quantify the oral infection. 2. Evaluate the progress during PBSCT. 3. Establish the oral bacteria detecting system from peripheral blood.1. There 3 items were necessary to plan dentistry treatment of the PBSCT patients. Measurement of periodontal pocket depth, detection and quantification of periodontal pocket bacterial, and leukocyte quantification and qualification were important. 2. We had survey periodontal conditions and immune responses of 12 patients before/during/after PBSCT. Although there were differences in periodontal condition, or immune system responses, we succeed the control of periodontal infection with all the patients. 3. We established the oral bacterial detecting system. Using this system, we became possible to detect and quantify oral bacterial without time-consuming cultivation.

在外周血干细胞移植(PBSCT)过程中，对受损患者的口腔感染进行控制，对于防止感染扩散到其他地区是非常重要的。我们应该清除牙周病细菌，但有可能未经考虑的治疗会使血管系统暴露在口腔细菌中。本研究的目的主要有以下三点。1、设置筛查参数项，量化口腔感染情况。2.评估外周干细胞移植的进展情况。3.建立外周血口腔细菌检测系统。计划PBSCT患者的牙科治疗需要3项内容。牙周袋深度的测量、牙周袋细菌的检测和定量、白细胞的定量和鉴定是重要的。2.对12例患者进行了PBSCT前、中、后牙周状况及免疫反应的调查。虽然在牙周状况或免疫系统反应方面有所不同，但我们成功地控制了所有患者的牙周感染。3.建立了口腔细菌检测系统。使用这个系统，我们能够检测和量化口腔细菌，而不需要耗时的培养。

项目成果

工藤 値英子: "歯周病治療により炎症マーカーが改善したと考察された重度歯周炎を伴うリウマチ患者の症例報告"日歯保存誌. 46. 110-117 (2003)

Eiko Kudo：“患有严重牙周炎的风湿病患者的病例报告，其炎症标志物被认为通过牙周病治疗得到了改善”日本牙科保护杂志 46. 110-117 (2003)。

Hiroshi Maeda et al.: "Quantitative Real-Time PCR Using TaqMan and SYBR Green system for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, TetQ gene and Total Bacteria"FEMS Immunol Med Microbiol. (投稿中).

Hiroshi Maeda 等人：“使用 TaqMan 和 SYBR Green 系统对放线放线杆菌、牙龈卟啉单胞菌、中间普氏菌、TetQ 基因和总细菌进行定量实时 PCR”FEMS 免疫医学微生物学（正在进行中）。

Maeda et al: "Quantitative real-time PCR using TaqMan and SYBR Green system for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, TetQ gene and total Bacteria"FEMS Immunol Med Microbiol. (to contribute).

Maeda 等人：“使用 TaqMan 和 SYBR Green 系统对放线放线杆菌、牙龈卟啉单胞菌、中间普雷沃氏菌、TetQ 基因和总细菌进行定量实时 PCR”FEMS 免疫医学微生物学。

Kudo et al: "A case report of Rheumatoid arthritis patient with severe periodontitis whose inframmatory marker improved by periodontal treatment"Japanese Journal of Conservative Dentistry. 46(1). 110-117 (2003)

Kudo等人：“类风湿性关节炎合并严重牙周炎患者的病例报告，其炎症标志物通过牙周治疗得到改善”《日本保守牙科杂志》。

Chiyo Fujimoto et al.: "Application of denaturing gradient gel electrophoresis (DGGE) to the analysis of microbiol communities of subgingival plaque"Journal of Periodontal Research. (in press). (2003)

Chiyo Fujimoto 等人：“应用变性梯度凝胶电泳 (DGGE) 分析龈下菌斑微生物群落”牙周研究杂志。