Development and application of high-performance electrochemical sensors for detecting biologically active substances
检测生物活性物质的高性能电化学传感器的开发与应用
基本信息
- 批准号:13672254
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The development and application of electrochemical sensors including ion selective electrode continue to be an area of focus. In this study, we developed new organic ammonium ion selective electrodes using homooxacalix[3]arene and calixarene derivatives. These derivatives with C_3 or C_6 symmetry are known to be effective in the recognition of primary organic ammonium ions. Since primary ammonium ions act as C_3-symmetrical proton donors, receptors bearing a C_3 symmetrically arranged proton acceptor site can interact with primary ammonium ions. Moreover, the hydrophobic cavities generated by the aromatic walls of phenol residues of these derivatives are potentially useful for the inclusion of non polar moieties of organic ammonium ions. In the process of screening homooxacalix[3]arene and calix[6]arene derivatives for responses to various organic ammonium ions including biogenic amines, we found that tris(methoxyphenylpropyloxy)hexahomooxacalix[3]arene-triethyl ether, responds strongly to serotonin, while p-tert-butylcalix[6]arene-hexaacetic acid hexaethyl ester responds to ethylammonium. This suggested that the cavity of homooxacalix[3]arene was spread out enough to interact with large molecules such as serotonin, while the cavity of calix[6]arene was so small, leading to effective interaction with ethylammonium. We also found that calix[8]arene, having larger cavity than calix[6]arene, responded to biologically active amines having a complicated structure, such as norephedrine. Calix[8]arene derivatives made effective recognition of bulky and complicated organic ammonium ions, using a large cavity and choosing an appropriate position at the proton acceptor sites to interact with these primary amines.We also applied ion selective electrodes to analyze the interaction between polyamines and the outer membrane of Escherichla coli cells. This and other results obtained in this study can be seen through references described in the back of this report.
包括离子选择电极在内的电化学传感器的开发和应用一直是人们关注的热点。在这项研究中,我们开发了一种新的有机铵离子选择电极,该电极采用均半杯芳烃和杯芳烃衍生物。这些具有C_3或C_6对称性的衍生物在识别原生有机铵离子方面是有效的。由于原铵离子作为C_3对称的质子供体,具有C_3对称排列的质子受体位点的受体可以与原铵离子相互作用。此外,这些衍生物的酚残基芳香族壁产生的疏水空腔对有机铵离子的非极性部分的包合有潜在的作用。在筛选同质杯[3]芳烃和杯[6]芳烃衍生物对各种有机铵离子(包括生物胺)的响应过程中,我们发现三(甲氧基苯基丙氧基)六同质杯[3]芳烃-三乙醚对5 -羟色胺反应强烈,而杯[6]芳烃-六乙酸六乙酯对乙胺反应强烈。这说明同萼[3]芳烃的空腔足够分散,可以与5 -羟色胺等大分子相互作用,而杯[6]芳烃的空腔很小,可以与乙胺有效相互作用。我们还发现杯[8]芳烃比杯[6]芳烃具有更大的空腔,对结构复杂的生物活性胺(如去甲麻黄碱)有反应。杯状[8]芳烃衍生物利用大腔体并在质子受体位点选择合适的位置与这些伯胺相互作用,有效地识别了体积大且复杂的有机铵离子。我们还应用离子选择电极分析了多胺与大肠杆菌细胞外膜的相互作用。这和本研究中获得的其他结果可以通过本报告后面描述的参考文献看到。
项目成果
期刊论文数量(14)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takashi Katsu: "Comparative study of the response of membrane electrodes based on calix[6]arms and calix[8]arene derivatives to organic ammonium ions"Anal.Sci.. 19(5). 771-774 (2003)
Takashi Katsu:“基于杯[6]臂和杯[8]芳烃衍生物的膜电极对有机铵离子的响应的比较研究”Anal.Sci.. 19(5)。
- DOI:
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- 影响因子:0
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Keiko Yasuda: "Mode of action of novel polyamines increasing the permeability of bacterial outer membrane"International Journal of Antimicrobial Agents. (in press). (2004)
安田惠子:“新型多胺增加细菌外膜渗透性的作用模式”国际抗菌剂杂志。
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- 影响因子:0
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Takashi Katsu: "Thallium(I)-selective membrane electrodes based on calix[6]arene or calix[5]arene derivatives"Sensors and Actuators B. 87. 331-335 (2002)
Takashi Katsu:“基于杯[6]芳烃或杯[5]芳烃衍生物的铊(I)选择性膜电极”Sensors and Actuators B. 87. 331-335 (2002)
- DOI:
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- 影响因子:0
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Takashi Katsu: "Interaction between polyamines and bacterial, outer membranes as investigated with ion-selective electrodes"Antimicrob.Agents Chemother.. 46(4). 1073-1079 (2002)
Takashi Katsu:“用离子选择性电极研究多胺与细菌外膜之间的相互作用”Antimicrob.Agents Chemother.. 46(4)。
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- 影响因子:0
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- 通讯作者:
Takashi Katsu: "Ethylammonium-selective membrane electrode using p-tert-butylcalix[6]arene derivatives"Analytical Sciences. 18. 473-476 (2002)
Takashi Katsu:“使用对叔丁基杯[6]芳烃衍生物的乙基铵选择性膜电极”分析科学。
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TAKASHI Katsu其他文献
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