权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Endocrine Disrupting Effects of Airborne Particle Matter and Their Responsible Constituents

空气颗粒物及其主要成分的内分泌干扰作用

基本信息

批准号：
13672342
负责人：
KIZU Ryoichi
金额：
$ 0.7万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672342/
关键词：
airborne suspended participate matter antiestrogenic effect antiandrogenic effect antagonist aryl hydrocarbon receptor polycyclic aromatic hydrocarbon MCF-7 cell PC3 cell 多環芳番族炭化水素 PC-3細胞 α-ナフトフラボンディーゼル車排気粉じんエストロゲン様作用

项目摘要

There is an increasing environmental concern on exogenious chemicals that exhibit adverse effects on endocrine systems of human and wild life, so-called endocrine disrupting substances. Endocrine disrupting effects of airborne suspended particulate matter (SPM) has also attracted much attention. Therefore, wecollected SPM in Kanazawa, Ishikawa, and Sapporo, Hokkaido, at April, July, October and January. Then, we assayed their endocrine disrupting effects in cultured human-originated cell lines and investigated the responsible constituents. The results obtained are described below. 1. Everey SPM samples exhibited the antiestrogenic and antiandrogenic effects. 2. At both Kanazawa and Sapporo, SPM samples showed greater effect in winter than in summer. This was considered to be due to increased energy consumption in winter compared with that in summer. 3. The endocrine disrupting effects of SPM samples were redued when the cells were treated with SPM samples in combination with an aryl hydrocarbon receptor (AhR) agonist. This results indicated that the endocrine disrupting effects were due in part to constituents acting as an AhR agonists. 4. Since polycyclic aromatic hydrocarbons (PAHs) are typical AhR agonists in environment, several PAHs were determined for their concentration in SPM samples and assayed for their endocrine disrupting effects. The results revealed that the endocrine disrupting effects of SPM samples are based in part on PAHs. 5. Competitive hormone receptor binding assay and yeast two-hybrid assay revealed that SPM samples contains the constituents capable to bind to estrogen receptor or androgen receptor as antagonists.

外源性化学物质对人类和野生动物的内分泌系统产生不利影响，即所谓的内分泌干扰物质，已引起越来越多的环境关注。大气悬浮颗粒物（SPM）的内分泌干扰效应也引起了广泛关注。因此，我们在金泽、石川和北海道的札幌分别于4月、7月、10月和1月采集SPM。然后，我们在培养的人源性细胞系中测定了它们的内分泌干扰作用，并调查了负责的成分。所得结果如下所述。1.各样品均具有抗雌激素和抗雄激素作用。2.在金泽和札幌，SPM样本显示出更大的影响，在冬季比夏季。这被认为是由于与夏季相比，冬季的能源消耗增加。3.当细胞用SPM样品与芳烃受体（AhR）激动剂组合处理时，SPM样品的内分泌干扰作用降低。这一结果表明，内分泌干扰作用部分是由于作为AhR激动剂的成分。4.由于多环芳烃（PAHs）是环境中典型的AhR激动剂，因此测定了SPM样品中多环芳烃的浓度，并研究了其内分泌干扰效应。结果表明，SPM样品的内分泌干扰效应部分是基于多环芳烃。5.竞争性激素受体结合试验和酵母双杂交试验表明，SPM样品中含有能够与雌激素受体或雄激素受体结合的拮抗剂成分。