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A study on microbial flora and environmental factors related to selerotium grain formation

与硒粒形成相关的微生物区系和环境因素研究

基本信息

批准号：
13680102
负责人：
WATANABE Makiko
金额：
$ 2.37万
依托单位：
TOKYO INSTITUTE OF TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680102/
关键词：
sclerotium grains microbial flora forest soils soil properties density of selerotium grains aluminum 菌核様粒子活性アルミニウム細菌フロラ解析 X線元素分析表面構造元素分析

项目摘要

Sclerotium grains, the resting bodies of ectomycorhizal fungi, are formed in forest soils as black spherical structures protected by thick and melanin-pigmented walls. Microbial flora and environmental factors related to the formation of sclerotium grains were studied to elucidate the symbiotic function of selerotium grain development in soil ecosystem. The content and chemical binding states of the major elements, C, Al, and O, in sclerotium gains were examined by X-ray photoelectron spectroscopy analysis. As for the experiments on the microbial flora inside the grains, the expected isolation of C. geophilum was not yet successful and most of the isolates were unknown species except strains relative to Rhodotorula mucilaginosa spp. Bacterial community analysis revealed the predominance of Alphaproteobacteria, chiefly the Sphingomonas group. It was unlikely that our Sphingomonas isolates had remarkable tolerance to aluminum toxicity. The relationships between the distribution of fungal sclerotium grains and soil chemical properties were studied in forest soils of Podsole, Braunfahlerde and Braunerde-Podsoles in Harz Mts., Germany and in volcanic ash soils of Mt. Myoko, and Mt. Akita-Komagatake, Central Japan. The formation of sclerotium grains in various forest soils of the Harz Mts. was strongly regulated by the state of active aluminum and the content of toxic Al (Al3+) as confirmed in Japanese Andosols. The relationship between the existence of aluminum and selerotium development was elucidated regardless of the soil type and the strength of soil acidity.

菌核颗粒是外生菌根真菌的静息体，在森林土壤中形成黑色球形结构，由厚实的黑色素壁保护。通过对菌核颗粒形成相关的微生物区系和环境因子的研究，阐明菌核颗粒发育在土壤生态系统中的共生功能。用x射线光电子能谱法测定了菌核中主要元素C、Al、O的含量和化学结合状态。在籽粒内微生物区系的实验中，预计分离到的C. geophilum尚未成功，除了与粘液红杆菌（Rhodotorula mucilaginosa spp）相关的菌株外，大多数菌株都是未知种。细菌群落分析显示，α变形菌属（Alphaproteobacteria）占优势，主要是鞘氨单胞菌（Sphingomonas）群。我们的鞘氨单胞菌不太可能对铝毒性有显著的耐受性。研究了德国哈茨山Podsole、Braunfahlerde和Braunerde-Podsoles森林土壤以及日本中部Myoko山和秋田- komagatake山火山灰土壤中真菌菌核颗粒的分布与土壤化学性质的关系。日本安土中已证实，哈尔茨地区不同森林土壤菌核颗粒的形成受活性铝状态和有毒铝（Al3+）含量的强烈调控。阐明了不论土壤类型和土壤酸度强弱，铝的存在与硒的发育之间的关系。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Distribution and development of sclerotium grains as influenced by aluminum status in volcanic ash soils

火山灰土壤中铝含量对菌核颗粒分布和发育的影响

DOI：
发表时间：
2002
期刊：
Sci.Plant Nutr. 48
影响因子：
0
作者：
Watanabe;M.;Kado;T.;Ohta;H.;Fujitake;N.
通讯作者：
N.

非アゴフェン黒ボク土における土壌菌核粒子の分布と土壌化学性との関係

非阿戈芬黑土土壤菌核颗粒分布与土壤化学的关系

DOI：
发表时间：
2004
期刊：
ペドロジスト 48
影响因子：
0
作者：
坂上伸生;渡邊眞紀子;太田寛行;藤嶽暢英
通讯作者：
藤嶽暢英

Phenolic compounds-degrading Sphingomonas spp. in fungal sclerotium-like grains from Mt. Myoko (Japan) volcanic ash soil

酚类化合物可降解鞘氨醇单胞菌。

DOI：
发表时间：
2003
期刊：
Abstracts of 16th International Symposium on Environmental Boigeochemistry 11
影响因子：
0
作者：
Ohta;H.;Yagi;M.;Suzuki;J.;Fujitake;N.;Watanabe;M.
通讯作者：
M.

Morphology, chemical properties and distribution of sclerotium grains studied in forest soils

森林土壤菌核颗粒的形态、化学性质和分布研究

DOI：
发表时间：
2003
期刊：
Abstracts of 16th International Symposium on Environmental Boigeochemistry 11
影响因子：
0
作者：
Watanabe;M.;Fujitake;N.;Ohta;H.
通讯作者：
H.

Status of aluminum and humus as the regulating factors of the growth of sclerotium grain in forest soils, Japan

日本森林土壤中铝和腐殖质作为菌核粒生长调节因子的现状

DOI：
发表时间：
2004
期刊：
Proceedings of 6th International Symposium on Plant-Soil Interaction at Low pH
影响因子：
0
作者：
Sakagami;N.;Watanabe;M.;Ohta;H.;Fujitake;N.
通讯作者：
N.