Action spectrum of UV-induced mutation in skin

紫外线诱发皮肤突变的作用谱

• 批准号: 13680617
• 负责人: IKEHATA Hironobu
• 金额: $ 2.69万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680617/
• 关键词: mutation; UV; skin; transgenic mouse; skin cancer; statospheric ozone layer; 皮膚がん

It is important to determine the action spectrum of ultraviolet light (UV)-induced mutation in skin for risk evaluation of sunlightinduced skin cancer in human population because the recent increase of the UV component of sunlight has caused significant induction of human skin carcinoma. Utilizing a bacterial lacZ gene-introduced transgenic mouse Muta^<TM>, which has been widely used for in vivo assay of induced mutations in an animal body exposed to various mutagens, we studied the induced mutations in mouse dorsal skin irradiated with monochromatic UV light at various wavelengths ranging around UVB and UVA regions. We showed the UV lights less than or equal to 334 nm could increase dose-dependently the frequency of the lacZ mutants in skin epidermis genome. The obtained action spectrum of mutation induction in UV-irradiated skin epidermis showed the maximum efficiency of mutation induction at the wavelengths shorter than or equal to 295 nm and 10,000-fold differences between the maximum and that at 334 nm. These results indicate the major contribution of UVB to skin cancer with the sunlight exposure of human skin and enable an quantitative evaluation of the risk of skin cancer in human population exposed to sunlight of known dose.

由于最近日光中紫外线成分的增加已经引起了人类皮肤癌的显着诱发，因此确定紫外线在皮肤中诱发突变的作用光谱对于评估人类日光诱发皮肤癌的风险是很重要的。利用细菌LacZ基因导入的转基因小鼠Muta^&lt；TM&gt；，研究了单色紫外光照射小鼠背部皮肤后UVB和UVA区附近不同波长的诱变。我们发现，小于或等于334 nm的紫外光可以剂量依赖性地增加皮肤表皮基因组中LacZ突变体的频率。经紫外线照射的皮肤表皮的诱变作用谱显示，295 nm以下波长的诱变效率最高，与334 nm的最大诱变效率相差10,000倍。这些结果表明，紫外线对皮肤癌的主要贡献是皮肤暴露在阳光下，并使人们能够定量评估暴露在已知剂量的阳光下的皮肤癌风险。

项目成果

Hironobu Ikehata: "Thermolysin improves mutation analysis in skin epidermis from ultraviolet light-irradiated Muta^<TM> mouse"Environmental and Molecular Mutagenesis. 38. 55-58 (2001)

Hironobu Ikehata：“嗜热菌素改善了紫外线照射的 Muta^<TM> 小鼠皮肤表皮的突变分析”环境和分子诱变。

Dcehata,H., S,.Aiba, H.Ozawa, T.Ono: "Thermolysin improves mutation analysis in skin epidermis from ultraviolet light-irradiated Muta^<TM> Mouse"Environ. Mol. Mutagen.. 38. 55-58 (2001)

Dcehata,H., S,.Aiba, H.Ozawa, T.Ono：“Thermolysin 改善了紫外线照射的 Muta^<TM> 小鼠皮肤表皮的突变分析”Environ。

Hironobu Ikehata: "Analysis of mutation spectrum in UVB-exposed mouse skin epidermis and dermis : frequent occurrence of C -> T transition at methylated CpG-associated dipyrimidine sites"Environmental and Molecular Mutagenesis. (発表予定). (2003)

Hironobu Ikehata：“UVB 暴露的小鼠皮肤表皮和真皮中的突变谱分析：甲基化 CpG 相关联嘧啶位点频繁发生 C -> T 转变”环境和分子诱变（即将发表）。

Hironobu Ikehata: "Thermolysin improves mutation analysis in skin epidermis from ultraviolet light-irradiated Muta^<TM> Mouse"Environmental and Molecular Mutagenesis. 30. 55-58 (2001)

Hironobu Ikehata：“嗜热菌素改善了紫外线照射的 Muta^<TM> 小鼠皮肤表皮的突变分析”环境和分子诱变。

Hironobu Ikehata: "Mutation induction with UVB in mouse skin epidermis is suppressed in acute high-dose exposure"Mutation Research. 508. 41-47 (2002)

Hironobu Ikehata：“小鼠皮肤表皮中 UVB 的突变诱导在急性高剂量暴露中受到抑制”突变研究。