A study to clarify the role of postsynaptic cells in synaptic plasticity by using double patch clamp method.
利用双膜片钳方法阐明突触后细胞在突触可塑性中的作用的研究。
基本信息
- 批准号:13680735
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have been studying the role of postsynaptic CaMKII during development of synapses at Drosophila neuromuscular junctions by using the system which consists of two muscles innervated by the same neurons. Activity of CaMKII can be manipulated in one of the two muscles or both muscles by GAL4-UAS expression system. We simultaneously recorded the response of those adjacent postsynaptic muscle cells by the double-patch clamp method. This seems to be a unique way to detect the specific effect of postsynaptic changes. We have found that postsynaptic activation of CaMKII promotes coordinated maturation of pre- and postsynaptic sites of newly hatched larvae in a synapse-specific manner. We also showed that its promotive effects were reduced in the older larvae which were about 7 hrs after hatching. On the other hand, synapses on the muscle cell in which CaMKII activity was not manipulated were affected in the older larvae. These results suggest that the action of CaMKII changes during synaptic development and the propagation of synaptic modification from-one synapse to the other appear only after a certain developmental stage of synapses.
我们一直在研究突触后CaMK Ⅱ在果蝇神经肌肉接头突触发育过程中的作用,通过使用系统,其中包括两个肌肉由相同的神经元支配。通过GAL 4-UAS表达系统可以在两块肌肉之一或两块肌肉中操纵CaMK II的活性。采用双膜片钳技术同时记录相邻突触后肌细胞的反应。这似乎是一种独特的方法来检测突触后变化的具体影响。我们已经发现,突触后激活的CaMKII促进协调成熟的前和突触后网站的新孵化的幼虫在突触特异性的方式。我们还发现,它的促进作用在孵化后约7小时的老幼虫中降低。另一方面,突触上的肌细胞中的CaMKII活性不受操纵的影响,在老年幼虫。这些结果表明,在突触发育过程中,CaMK Ⅱ的作用发生了变化,突触修饰从一个突触传播到另一个突触,只有在突触发育到一定阶段后才出现。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kazama, H., Morimoto Tanifuji, T., Nose, A.: "Postsynaptic Activation of CaMKH Promotes Coordinated Pre and Postsynaptic Maturation of Drosophila Neuromuscular Junctions"Neuroscience. 117・3. 615-625 (2003)
Kazama, H.、Morimoto Tanifuji, T.、Nose, A.:“CaMKH 的突触后激活促进果蝇神经肌肉接头的突触前和突触后协调成熟”神经科学 117・3。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kazama, H., Morimoto-Tanifuji, T., and Nose A.: Neuroscience. 117-3. 615-625 (2003)
Kazama, H.、Morimoto-Tanifuji, T. 和 Nose A.:神经科学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
TANIFUJI Takako其他文献
TANIFUJI Takako的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
相似海外基金
Investigating a novel role of DRAK2 in T cell migration and synapse formation
研究 DRAK2 在 T 细胞迁移和突触形成中的新作用
- 批准号:
10680274 - 财政年份:2023
- 资助金额:
$ 2.3万 - 项目类别:
A synthetic biosensor of immunologic synapse formation allowing multiplexed T cell antigen discovery for autoimmune neurologic disorders
一种免疫突触形成的合成生物传感器,可发现自身免疫性神经系统疾病的多重 T 细胞抗原
- 批准号:
10740610 - 财政年份:2023
- 资助金额:
$ 2.3万 - 项目类别:
Identification of genes involved in photoreceptor recognition and synapse formation
鉴定参与光感受器识别和突触形成的基因
- 批准号:
10766366 - 财政年份:2023
- 资助金额:
$ 2.3万 - 项目类别:
Molecular mechanism of inhibitory synapse formation
抑制性突触形成的分子机制
- 批准号:
22K06805 - 财政年份:2022
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidating the functions of zonula occludens (ZO) proteins in regulating chemical synapse formation
阐明闭合带 (ZO) 蛋白在调节化学突触形成中的功能
- 批准号:
486398 - 财政年份:2022
- 资助金额:
$ 2.3万 - 项目类别:
Studentship Programs
Molecular mechanisms of human synapse formation induced by secreted bloodborne factors
血源性分泌因子诱导人突触形成的分子机制
- 批准号:
DGECR-2022-00219 - 财政年份:2022
- 资助金额:
$ 2.3万 - 项目类别:
Discovery Launch Supplement
Molecular mechanisms of human synapse formation induced by secreted bloodborne factors
血源性分泌因子诱导人突触形成的分子机制
- 批准号:
RGPIN-2022-04532 - 财政年份:2022
- 资助金额:
$ 2.3万 - 项目类别:
Discovery Grants Program - Individual
Investigating mechanisms of neuronal growth and synapse formation
研究神经元生长和突触形成的机制
- 批准号:
RGPIN-2019-06332 - 财政年份:2022
- 资助金额:
$ 2.3万 - 项目类别:
Discovery Grants Program - Individual
Molecular mechanisms of target-specific synapse formation
靶标特异性突触形成的分子机制
- 批准号:
10184919 - 财政年份:2021
- 资助金额:
$ 2.3万 - 项目类别:
The integrin-dependent B cell actomyosin network drives immune synapse formation and B cell functions
整合素依赖性 B 细胞肌动球蛋白网络驱动免疫突触形成和 B 细胞功能
- 批准号:
546047-2020 - 财政年份:2021
- 资助金额:
$ 2.3万 - 项目类别:
Postdoctoral Fellowships