40kDa-Binding Protein to Phytochrome and Light-signal Transduction

40kDa 光敏色素结合蛋白和光信号转导

• 批准号: 13680748
• 负责人: TOKUTOMI Satoru
• 金额: $ 2.3万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680748/
• 关键词: PHYTOCHROME; PHOTOMORPHOGENESISI; PHOTORECEPTOR; PHOTOREACTION; SIGNAL-TRANSDUCTION; BINDING-PROTEIN; 光環境情報; 光センシング

Phytochrome is a plant photoreceptor for variety of photomorphogenetic responses that has two UV-visible absorption forms, Pr and Pfr. Pfr is thought to be an active form. Phytochrome B, responsible for low fluence rate responses migrates into nucleus to bind an interacting protein, PIF3, on phototransformation from Pr to Pfr, that initiates transcription of flowering-related genes, however, signaling of phytochrome A is poorly understood. We have detected multiple binding proteins to Pfr of phytochrome A. We determined N-terminal amino acid sequences of several of these binding proteins. One of them, 40kDa species is identified as a homologue of the enzyme that is involved in the important process of IAA, a plant hormone, synthesis. We cloned the gene and expressed it. Enzymatic activities of the expressed products were measured, proposing its involvement in IAA synthesis. Its interaction with phytochrome A and possible photoregulation of its activities are to be investigated.

光敏色素是一种植物光感受器，具有两种紫外可见吸收形式，即Pr和Pfr。PFR被认为是一种活性形式。光敏色素B负责低通量反应，在从Pr到Pfr的光转化过程中迁移到细胞核结合相互作用的蛋白PIF3，启动开花相关基因的转录，但对光敏色素A的信号转导知之甚少。我们已经检测到多个与光敏色素A的PFR结合的蛋白，并测定了其中几个结合蛋白的N末端氨基酸序列。其中一个40 kDa的物种被鉴定为参与植物激素IAA合成的重要过程中的酶的同源物。我们克隆了这个基因并进行了表达。对表达产物的酶活性进行了测定，表明其参与了IAA的合成。它与光敏色素A的相互作用以及对其活性的可能的光调节还有待研究。

项目成果

徳富 哲: "植物のカラービジョン"化学と生物. 40巻1号. 61-65 (2002)

Satoshi Tokutomi：“植物的颜色视觉”化学与生物学，第 40 卷，第 1. 61-65 (2002)。

Iwata T., Nozaki., D., Tokutomi, S., Kagawa, T., Wada, M., Kandori, H.: "Light-Induced Structural Changes in the LOV2 Domain of Adiantum Phytochrome3 Studied by Low-Temperature FTIR and UV-Visible Spectroscopy"Biochemistry. 42. 8183-8191 (2003)

Iwata T.、Nozaki.、D.、Tokutomi, S.、Kakawa, T.、Wada, M.、Kandori, H.：“通过低温 FTIR 和研究研究了铁线蕨光敏色素 3 LOV2 结构域的光诱导结构变化”

Saitou, T., Hashidume, Ai., Tokutomi S., Kamada, H.: "Reduction of phytochrome level and light-induced formation of adventitous shoots by introduction of antisense genes for phytochrome A in horseradish hairy roots"Plant, Cell, Tissue & Organ Culture. vol

Saitou, T.、Hashidume, Ai.、Tokutomi S.、Kamada, H.：“通过在辣根毛状根中引入光敏色素 A 的反义基因，降低光敏色素水平和光诱导的不定芽形成”植物、细胞、组织

和田正三他: "植物細胞工学シリーズ16 植物の光センシング"秀潤社. 192 (2001)

Shozo Wada 等：《植物细胞工程系列 16 植物光学传感》Shujunsha 192 (2001)。

Saitou, T., Hashidume, Ai., Tokutomi, S., Kamada, H.: "Reduction of phytochrome level and light-induced formation of adventitious shoots by introduction of antisense genes for phytochrome A in horseradish hairy roots"Plant, Cell, Tissue & Organ Culture. 1

Saitou, T.、Hashidume, Ai.、Tokutomi, S.、Kamada, H.：“通过在辣根毛状根中引入光敏色素 A 的反义基因，降低光敏色素水平和光诱导的不定芽形成”植物、细胞、