A Unique Target in the Purine Biosynthesis of the Pathogen Helicobacter pylori

病原体幽门螺杆菌嘌呤生物合成的独特靶点

• 批准号: DP0345210
• 负责人: Prof George Mendz
• 金额: $ 8.63万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-02-06 至 2005-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0345210
• 关键词: Unique; Target; Purine; Biosynthesis; Pathogen

The uptake systems of purine and analogues of the human pathogen Helicobacter pylori will be characterised because they can be utilised to introduce cytotoxic compounds into the cells. The first step in de novo purine biosynthesis of the bacterium is catalysed by two different enzymes, which are components of other biosynthetic pathways. These unique properties make them excellent potential therapeutic targets. Their individual combined activities in purine biosynthesis will be characterised in situ and in vitro. Isogenic mutants with inactivated genes encoding for these enzymes will be constructed to investigate their role in the survival of the organism.

嘌呤和人类病原体幽门螺杆菌的类似物的摄取系统将被描述，因为它们可以被用来将细胞毒性化合物引入细胞。细菌从头合成嘌呤的第一步是由两种不同的酶催化的，这两种酶是其他生物合成途径的组成部分。这些独特的特性使它们成为极具潜力的治疗靶点。它们在嘌呤生物合成中的单独结合活性将在原位和体外进行表征。将构建编码这些酶的失活基因的等基因突变体，以研究它们在生物体生存中的作用。