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Molecular system ensuring genomic inheritance through successive generations

确保基因组遗传代代相传的分子系统

基本信息

批准号：
14208088
负责人：
KISHIMOTO Takeo
金额：
$ 34.2万
依托单位：
Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14208088/
关键词：
cell cycle control nuclear formation meiosis fertilization DNA replication signal transduction cyclin-CDK Mos-MAPK 前核形成 PI3キナーゼインポーチンα Mcm p90Rsk 染色体構築ヒトデ卵カエル卵

项目摘要

Molecular mechanisms that ensure genomic inheritance through successive generations have been studied, based on the cell cycle control and the nuclear formation. Our findings are summarized below.1. Meiotic cell cycle progression : Almost complete signaling pathway that leads to meiotic reinitiation in starfish oocytes has been revealed to be composed of the putative receptor for maturation-inducing hormone, 1-MeAde / Gβ1γ2 / I-β type PI3-kinase / PDK1 and putative PDK2 / Akt (PKB) / Cdc2-cyclin B / Plk1. After meiotic reinitiation, p90Rsk is activated immediately downstream of the Mos-MAPK pathway to regulate positively both meiosis I to II transition and G1-phase arrest after completion of meiosis II.2. Formation of male and female pronuclei : Cell-free extracts derived from Xenopus oocytes or eggs were utilized to analyze these processes. Upon fertiliztion, NAP1 functions as a molecular chaperone for histone H1 at the chromatin remodeling in sperm nuclei. During oocyte maturation, the ability of nuclear import that depends on importin α is accelerated, and the LI-type nuclear lamin is newly synthesized, possibly providing a clue to understand how the ability for nuclear formation is acquired in this period.3. Initiation of S-phase upon fertilization : In unfertilized mature eggs of starfish, MCMs are already loaded onto chromatin in female pronucleus, but the loading of Cdc45, which allows the loading of DNA polymerase a to DNA, is prevented downstream of the Mos-MAPK-p90Rsk pathway. Abolishment of p90Rsk activity is sufficient for initiation of DNA replication, thus providing a clue to answer the classic question in biology how fertilization initiates S-phase.

基于细胞周期控制和核形成，已经研究了确保基因组通过连续世代遗传的分子机制。我们的研究结果总结如下。减数分裂细胞周期进程：在海星卵母细胞中，几乎完整的导致减数分裂重新启动的信号通路已被揭示由成熟诱导激素的推定受体1-MeAde/G β 1 γ 2/I-β型PI3-激酶/PDK 1和推定的PDK 2/Akt（PK B）/Cdc 2-cyclin B/Plk 1组成。减数分裂重新启动后，p90Rsk在Mos-MAPK通路的下游立即被激活，以积极调节减数分裂I至II的转换和减数分裂II完成后的G1期阻滞。雄性和雌性原核的形成：利用来自非洲爪蟾卵母细胞或卵的无细胞提取物来分析这些过程。受精后，NAP1作为组蛋白H1的分子伴侣参与精子核染色质的重塑。在卵母细胞成熟过程中，依赖于importin α的核输入能力加快，LI型核纤层蛋白新合成，这可能为理解这一时期核形成能力的获得提供线索.受精后S期的启动：在海星未受精的成熟卵中，MCMs已经加载到雌性原核的染色质上，但允许DNA聚合酶a加载到DNA的Cdc45的加载被阻止在Mos-MAPK-p90Rsk通路的下游。p90Rsk活性的消除足以启动DNA复制，从而为回答生物学中受精如何启动S期的经典问题提供了线索。

项目成果

期刊论文数量（14）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

APC/C-Cdc2O-mediated degradation of cyclin B participates in CSF arrest in unfertilized Xenopus eggs.

APC/C-Cdc2O 介导的细胞周期蛋白 B 降解参与未受精非洲爪蟾卵中 CSF 的停滞。

DOI：
发表时间：
2005
期刊：
Dev.Biol. 279
影响因子：
0
作者：
Yamamoto;T. 他
通讯作者：
T. 他

Oocyte Extracts for the Study of meiotic M-M Transition.

用于研究减数分裂 M-M 转变的卵母细胞提取物。

DOI：
发表时间：
2006
期刊：
Methods Mol.Biol. 322
影响因子：
0
作者：
K.Ohsumi;T.M.Yamamoto;M.Iwabuchi
通讯作者：
M.Iwabuchi

Nucleosome assembly protein-1 is a linker histone chaperone in Xenopus eggs

DOI：
10.1073/pnas.0500822102
发表时间：
2005-06-07
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Shintomi, K;Iwabuchi, M;Ohsumi, K
通讯作者：
Ohsumi, K

More than G1 or G2 arrest : Useful starfish oocyte system for investigating skillful MAP kinase (Review).

超过 G1 或 G2 停滞：用于研究熟练 MAP 激酶的有用海星卵母细胞系统（评论）。