Separation of Hydrolysate Amino Acids by Electrodialysis with a Bioplar Membrane and Ion-exchange Membranes

使用生物膜和离子交换膜通过电渗析分离水解产物氨基酸

• 批准号: 14550746
• 负责人: TAKAHASHI Hiroshi
• 金额: $ 2.37万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550746/
• 关键词: bipolar membrane; electrodialysis; ion-exchange membrane; pH step; amino acid; pH勾配; タンパク質加水分解

We have developed a new technique of electrodialysis with a bipolar membrane and ion-exchange membranes. The technique has a separating zone with pH steps between compartments, and may be possible to separate ampholytes by difference of the isoelectric points. The electrodialyzer composed of six compartments, which were divided by a cation-exchange membrane, a bipolar membrane, and anion-exchange membranes, respectively. When a voltage was applied to the electrodialyzer, hydroxide ions were electrically generated by the anion-exchange layer in the bipolar membrane, and moved to anode side compartments through anion-exchange membranes. The hydroxide concentration in each compartment was almost equall to the concentration of coexisting cations, and formed alkaline-pH steps in the electrodialyzer. Moreover, we attempted to apply the bipolar membrane technique to separation of acidic and neutral amino acids. In the range of pH steps of 8 to 13, acidic amino acid permeated through the anion-exchange membranes, and accumulated in the anode compartment, although neutral amino acids remained in the feed compartment. Thus, the separation by the new technique proceeded well. All the experimental data were simulated by a mathematical model, which considers dissociation of amino acids, electro-neutrality in the solution, ion-exchange equilibrium, hydroxide production rate with a bipolar membrane, and flux in the anion-exchange membranes. The model well explained the experimental results of the formation of pH steps in the electrodialyzer, and of the amino acids separation.

我们开发了一种新的电渗析技术与双极膜和离子交换膜。该技术具有在隔室之间具有pH阶跃的分离区，并且可以通过等电点的差异来分离两性电解质。电渗析器由六个隔室组成，分别由阳离子交换膜、双极膜和阴离子交换膜分隔。当向电渗析器施加电压时，氢氧离子通过双极膜中的阴离子交换层电生成，并通过阴离子交换膜移动到阳极侧隔室。各室中氢氧化物的浓度与共存阳离子的浓度几乎相等，并在电渗析器中形成碱性-pH台阶。此外，我们还尝试将双极膜技术应用于酸性氨基酸和中性氨基酸的分离。在pH值为8 ~ 13的范围内，酸性氨基酸透过阴离子交换膜，在阳极室中积累，而中性氨基酸则保留在进料室中。因此，通过新技术的分离进行得很好。所有的实验数据进行了模拟的数学模型，该模型考虑了氨基酸的解离，在溶液中的电中性，离子交换平衡，氢氧化物的生产率与双极膜，和通量的阴离子交换膜。该模型很好地解释了实验结果的形成pH台阶的电渗析器，和氨基酸的分离。

项目成果

高橋博, 牧田健吾, 菊地賢一: "バイポーラ膜併用型電気透析による中性アミノ酸の相互分解離"化学工学会第36回秋季大会講演要旨集V2A08. (2003)

Hiroshi Takahashi、Kengo Makita、Kenichi Kikuchi：“双极膜联合电渗析对中性氨基酸的相互分解”第 36 届化学工程师学会秋季会议摘要（2003 年）。

バイポーラ膜併用型電気透析におけるアルカリpH勾配の形成とアミノ酸の膜透過特性

双极膜电渗析中碱性pH梯度的形成及氨基酸的膜通透特性

• 发表时间: 2002
• 期刊: 化学工学会第67年会講演要旨集 I105
• 作者: 牧田健吾;高橋博;菊地賢一
• 通讯作者: 菊地賢一

Formation of Alkaline pH steps in an electrodialysis with a Bipolar and Ion-exchange Membranes, and Its Application to Amino Acids Separation

双极性离子交换膜电渗析中碱性 pH 阶跃的形成及其在氨基酸分离中的应用

• 发表时间: 2004
• 期刊: Proc.of 10^<th> The Asian Pacific Confederation of Chemical Engineers 1P-12-014
• 作者: Hiroshi Takahashi;K.Makita;K-I.Kikuchi
• 通讯作者: K-I.Kikuchi

Formation of Alkaline pH Steps in an Electrodialyzer with a Bipolar and Ion-exchange Membranes, and Its Application to Amino Acids Separation

双极性离子交换膜电渗析器中碱性 pH 阶跃的形成及其在氨基酸分离中的应用

• 发表时间: 2004
• 期刊: Proc.of 10^<th> The Asian Pacific Confederation of Chemical Engineers 1P-12-014
• 作者: Hiroshi Takahashi;K.Makita;K-I.Kikuchi
• 通讯作者: K-I.Kikuchi

バイポーラ膜併用型電気透析における中性アミノ酸の相互分離

双极膜电渗析中性氨基酸的相互分离

• 发表时间: 2003
• 期刊: 化学工学会第36回秋季大会講演要旨集 V2A08
• 作者: 高橋博;牧田健吾;菊地賢一
• 通讯作者: 菊地賢一