Molecular Imprinted Biomass for the Separation of Metal Ions

用于分离金属离子的分子印迹生物质

• 批准号: 14550752
• 负责人: UEZU Kazuya
• 金额: $ 2.3万
• 依托单位: The University of Kitakyushu
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550752/
• 关键词: biosorption; molecular-recognizing material; molecular imprinting; microorganism; metal ion separation; biomass; function modification; adsorbent; バイオソープジョン

Adsorption performance of metal ion, lanthane, copper, and cobalt, by Rhizopus oligosporus (Rh.oligo.) fermentated in liquid media were investigated. Rh.oligo. P12 demonstrated the highest metal-adsorption amount, whose amount of metal adsorbed was 3.2 mmol/g-mycelia, among the Rh.oligo. species, which was comparable to the functional beads for conventional wastewater treatments. From the analysis of Freundlich and Scatchard plots, the several functional groups of Rh.oligo. P12 were concerned with the adsorption of metal. With increasing pH in metal solution, the amount of metal adsorbed was increased for lanthane and cobalt. The pH-dependence slope of lanthane and cobalt were 1/3 and 1/8, respectively, from which the interaction between metal and mycelia was speculated to be not via ionic interaction but via coordination interaction etc. The adsorption site of metal ions by Rh.oligo. would be their cell wall, and we have been modifying them by molecular imprinting for higher metal-adsorption fungi.

少孢根霉对稀土、铜、钴等金属离子的吸附性能在液体培养基中发酵。Rh.oligo. P12表现出最高的金属吸附量，其吸附量为3.2 mmol/g-菌丝体，在Rh. oligo。种，这是可比的功能珠常规废水处理。从Freundlich和Scatchard图的分析，几个功能团的Rh. oligo。P12与金属离子的吸附有关。随着金属溶液pH值的增加，镧和钴的吸附量增加。镧和钴的pH依赖性斜率分别为1/3和1/8，由此推测金属与菌丝体之间的相互作用不是通过离子相互作用，而是通过配位相互作用等。是它们的细胞壁，我们一直在通过分子印迹对它们进行修饰，以获得更高的金属吸附真菌。

项目成果

Eiichi Toorisaka, Kazuya Uezu, Masahiro Gito, Shintaro: "A molecular imprinted polymer that shows enzymatic activity"Biochemical Engineering Journal. 14・2. 85-91 (2003)

Eiichi Toorisaka、Kazuya Uezu、Masahiro Gito、Shintaro：“显示酶活性的分子印迹聚合物”生物化学工程杂志 14・2（2003）。

Eiichi Toorisaka, Kazuya Uezu, Masahiro Goto, Shintaro Furusaki: "A molecular imprinted polymer that shows enzymatic activity"Biochamical Engineering Journal. 14. 85-91 (2003)

Eiichi Toorisaka、Kazuya Uezu、Masahiro Goto、Shintaro Furusaki：“显示酶活性的分子印迹聚合物”生物化学工程杂志。

Tatsuya Oshima, Katsutoshi Inoue, knzuya Uezu, MaSahiro Goto: "Dominant factors affecting extraction behavior of amino compounds by a calix [6]arene carboxylic acid derivative"Analytica Chimica Acta. 509・2. 137-144 (2004)

Tatsuya Oshima、Katsutoshi Inoue、knzuya Uezu、MaSahiro Goto：“影响杯[6]芳烃羧酸衍生物提取氨基化合物行为的主要因素”Analytica Chimica Acta 509・2。

Hiroshi Morita, Masami Shiozawa, Yusaku Fujio: "Ribonuclease production of Rhizopus oryzae IFO 4697 under metal ion stress in liquid culture"Mycoscience. 43. 283-287 (2002)

Hiroshi Morita、Masami Shiozawa、Yusaku Fujio：“液体培养物中金属离子胁迫下米根霉 IFO 4697 的核糖核酸酶生产”Mycoscience。

Tatsuya Oshima, Katsutoshi Inoue, Kazuya Uezu, Masahiro Goto: "Dominant factors affecting extraction behavior of amino compounds by a calyx[6]arene carboxylic acid derivative"Analytica Chimica Acta. 509. 137-144 (2004)

Tatsuya Oshima、Katsutoshi Inoue、Kazuya Uezu、Masahiro Goto：“影响花萼[6]芳烃羧酸衍生物提取氨基化合物行为的主要因素”分析化学学报。