Detection system for the influenza virus by novel functional polymer and artificial enzyme

新型功能聚合物和人工酶流感病毒检测系统

• 批准号: 23656495
• 负责人: UEZU Kazuya
• 金额: $ 2.58万
• 依托单位: The University of Kitakyushu
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23656495/
• 关键词: 分子認識; ウィルスセンサー; ポリマーブラシ; シグナル変換; 分離精製; 化学センサー; 環境分析

The hemagglutinin protein on influenza virus can recognize the sialic acid on the cell membrane, and the virus infects the human cell. In this study, we utilize this mechanism to prepare the functional membrane, which can detects the influenza virus. This functional membrane was prepared by radiation-induced graft polymerization. To immobilize the sialic acid as the recognition site of the hemagglutinin protein on the influenza virus, the coupled iminodiacetic acid and 1-ethyl-3-carbodiimide hydrochloride was introduced onto the porous hollow fiber membrane. Copper ion as a signal substance was adsorbed on the sialic acid immobilizesd porous hollow fiber membrane, and it indicated the possibility of the sensor for influenza virus.

流感病毒上的血凝素蛋白能识别细胞膜上的唾液酸，病毒感染人体细胞。本研究利用这一机制制备了检测流感病毒的功能膜。该功能膜是通过辐射接枝聚合制备的。为了将唾液酸作为流感病毒血凝素蛋白的识别位点，将偶联的亚氨基二乙酸和1-乙基-3-碳二亚胺盐酸盐引入到多孔中空纤维膜上。铜离子作为信号物质吸附在唾液酸固定的多孔中空纤维膜上，表明了作为流感病毒传感器的可能性。