Development of Green Bioreactor for Concentrated Xanthan Production

浓缩黄原胶生产绿色生物反应器的开发

• 批准号: 14550742
• 负责人: TERASAKA Koichi
• 金额: $ 2.18万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550742/
• 关键词: Xanthan; Thickening Agent; Viscous Liquid; Non-Newtonian Liquid; Oxygen Transfer; Bubble Column; Gas Holdup; Chemical Reactor Engineering; 非ニュートン液体; バイオリアクター; 降伏応力

Xanthan which is a natural product by Xanthomonas campestris is very useful thickener and additive for food. The production process for xanthan is usually utilized stirred tank bioreactors due to well fermentation in industry. However, the yield stress of culture medium increases rapidly with fermentation so that gas-liquid mixing in stirred tank becomes sometimes poor. Even if a power input for impeller increases to keep mixing well, the liquid far from the impeller becomes stagnant as well as the fungi may be damaged by large shear stress dose to the impeller. When the fungi die due to shortage of oxygen in medium, the xanthan fermentation finishes. For dilute concentration of xanthan gum produced in the solution, large evaporation or separation processes must be required in separation and purification process. In those downstream process, a large amount of waste solutions generates.On the other hand, bubble columns are well known as good aerobic reactors whose mixture is done by bub … More ble convection. Moreover, bubble columns can be easily sterilized and scaling-up.In this study, the purpose is to develop a bubble column bioreactor for the higher concentrated xanthan production up to protect water environment. This study was proceeded as following steps :(1)Fermentation on agar(2)Fermentation in flask and small bubble column(3)Xanthan production in a stirred tank bioreactor.(4)Xanthan production in a standard bubble column(5)Development of novel bubble column bioreactor having high oxygen transferOxygen concentration decreased with fermentation in both stirred tank and standard bubble column. For the xanthan fermentation, the oxygen transfer into culture medium limited the xanthan production due to the liquid became very viscous. Of all bioreactors used in this study, the partitioned bubble column has the higher oxygen transfer coefficient even in the most viscous xanthan solution. Therefore, the proposed bubble column bioreactor is suitable more efficiently to produce xanthan than the other processes. Less

黄原胶是野油菜黄单胞菌的天然产物，是一种非常有用的食品增稠剂和添加剂。黄原胶的生产工艺通常采用搅拌槽式生物反应器，因为工业上发酵效果较好。然而，随着发酵的进行，培养基屈服应力迅速增大，有时搅拌槽内的气液混合变差。即使增加搅拌器的功率输入以保持良好的混合，远离搅拌器的液体也会因叶轮受到较大的剪应力剂量而变得停滞，真菌也会受到破坏。当真菌因培养基中的氧气不足而死亡时，黄原胶发酵结束。对于溶液中产生的稀释黄原胶，在分离纯化过程中必须进行大量的蒸发或分离过程。另一方面，鼓泡塔是众所周知的好氧反应器，它的混合是由BUB…完成的更明显的对流。此外，鼓泡塔易于杀菌和放大。本研究的目的是开发一种鼓泡塔生物反应器，用于生产更高浓度的黄原胶，以保护水环境。本研究按以下步骤进行：(1)琼脂发酵(2)摇瓶小鼓泡塔发酵(3)搅拌釜式生物反应器生产黄原胶(4)标准鼓泡塔生产黄原胶(5)新型高氧转移式鼓泡塔生物反应器的研制在黄原胶发酵中，由于液体粘稠度大，氧气向培养基内的转移限制了黄原胶的产量。在本研究使用的所有生物反应器中，隔板鼓泡塔即使在粘度最高的黄原胶溶液中也具有较高的氧气传递系数。因此，鼓泡塔生物反应器比其他工艺更适合生产黄原胶。较少

项目成果

Gas holdup for slug bubble flow of viscous liquids having a yield stress in bubble columns

气泡塔中具有屈服应力的粘性液体的段塞气泡流的含气率

• 发表时间: 2003
• 期刊: Chemical Engineering Science 58
• 作者: Koichi Terasaka;Hideki Tsuge
• 通讯作者: Hideki Tsuge

Oxygen Transfer in Viscous Non-Newtonian Liquids Having Yield Stress in Bubble Columns Operated under Slug Flow Condition

段塞流条件下操作的鼓泡塔中具有屈服应力的粘性非牛顿液体中的氧传递

• 发表时间: 2003
• 期刊: Chemical Engineering Science 58
• 作者: Koichi Terasaka;Koichi Terasaka
• 通讯作者: Koichi Terasaka

Koichi TERASAKA: "Gas holdup for slug bubble flow of viscous liquids having a yield stress in bubble columns"Chemical Engineering Science. 58. 513-517 (2003)

Koichi TERASAKA：“气泡塔中具有屈服应力的粘性液体的段状气泡流的气体含率”《化学工程科学》。

Gas holdup for slug bubble flow of viscous liquids having yield stress in bubble columns

气泡塔中具有屈服应力的粘性液体的段塞气泡流的含气率

• 发表时间: 2003
• 期刊: Chemical Engineering Science 58
• 作者: Koichi Terasaka

Koichi Terasaka: "Oxygen Transfer in Viscous Non-Newtonian Liquids Having Yield Stress in Bubble Columns Operated under Slug Flow Condition"Chemical Engineering Science. 58. 5331-5337 (2003)

Koichi Terasaka：“在团状流条件下操作的气泡塔中具有屈服应力的粘性非牛顿液体中的氧转移”化学工程科学。