Highly sensitive and highly accurate analysis by capillary electrophoresis

通过毛细管电泳进行高灵敏度和高精度分析

• 批准号: 14550782
• 负责人: HIROKAWA Takeshi
• 金额: $ 2.3万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550782/
• 关键词: Capillary electrophoresis; Microchip electrophoresis; On-line preconcentration; Transient isotachophoresis; Highly sensitive and highly accurate analysis; 過渡的等速電気泳動; キャピラリー電気泳動; 過渡的等速電気前濃縮; 電気的試料注入; 微量分析; 高精度分析; 高感度分析

An on-line preconcentration method utilizing transient isotachophoresis with electrokinetic injection for capillary zone electrophoresis (CZE) and microchip electrophoresis (MCE) was studied by means of computer simulation and experiments. Consequently it was found that this preconcentration method was very useful to realize highly sensitive and highly accurate analysis by CE and MCE, and named as electokinetic supercharging (EKS). Simulational results could be utilized efficiently for optimizing experimental conditions of EKS : The important points were appropriate selection of the leading electrolyte and the terminating one and their molar amount introduced to the separation system depending on the mobility of sample components and the sample amount. Existence of electroosmotic flow(EOF) was not favorable for preconcentration and it should be suppressed for better preconcentration efficiency. Confirming these simulational results by experiments, we achieved LOD of sub-ppb level for ions of alkaline metals, alkaline earth metals and rare-earth elements by EKS-CE, which is comparable with IC and ICP-AES. The proposed transient isotachophoretic method could be applied to e.g. river water with low ionic strength and also to e.g. seawater with high ionic strength. Analysis of iodide and iodate in seawater is a good example of the latter case. The method is also applicable to MCE : We could obtain LOD of 20 times as small as that of usual method (pinched injection) for the analysis of DNA fragments and proteins by EKS-MCE. These results were published in J.Chromatogr. and Electrophoresis and higly evaluated. A patent related to the proposed method ォ病s applied through JST

An on-line preconcentration method utilizing transient isotachophoresis with electrokinetic injection for capillary zone electrophoresis (CZE) and microchip electrophoresis (MCE) was studied by means of computer simulation and experiments. Consequently it was found that this preconcentration method was very useful to realize highly sensitive and highly accurate analysis by CE and MCE, and named as electokinetic supercharging (EKS). Simulational results could be utilized efficiently for optimizing experimental conditions of EKS : The important points were appropriate selection of the leading electrolyte and the terminating one and their molar amount introduced to the separation system depending on the mobility of sample components and the sample amount. Existence of electroosmotic flow(EOF) was not favorable for preconcentration and it should be suppressed for better preconcentration efficiency. Confirming these simulational results by experiments, we achieved LOD of sub-ppb level for ions of alkaline metals, alkaline earth metals and rare-earth elements by EKS-CE, which is comparable with IC and ICP-AES. The proposed transient isotachophoretic method could be applied to e.g. river water with low ionic strength and also to e.g. seawater with high ionic strength. Analysis of iodide and iodate in seawater is a good example of the latter case. The method is also applicable to MCE : We could obtain LOD of 20 times as small as that of usual method (pinched injection) for the analysis of DNA fragments and proteins by EKS-MCE. These results were published in J.Chromatogr. and Electrophoresis and higly evaluated. A patent related to the proposed method applied through JST

项目成果

Z.Q.Xu: "High-sensitive CGE analysis of DNA fragments on electrophoresis microchip using electrokinetic injection with transient isotachophoresis preconcentration"J. Chromatogr. A. 990. 53-61 (2003)

Z.Q.Xu：“使用动电注射和瞬时等速电泳预浓缩对电泳微芯片上的 DNA 片段进行高灵敏 CGE 分析”J。

Z.Q.Xu: "High-sensitive capillary gel electrophoretic analysis of DNA fragments on an electrophoresis microchip using electrokinetic injection with transient isotachophoresis preconcentration"J.Chromatogr.A. 990. 53-61 (2003)

Z.Q.Xu：“使用动电注射和瞬时等速电泳预浓缩对电泳微芯片上的 DNA 片段进行高灵敏度毛细管凝胶电泳分析”J.Chromatogr.A.

T.Hirokawa: "Trace ion analysis of seawater by capillary electrophoresis : determination of iodide using transient isotachophoretic preconcentration"Electrophoresis. 24. 2328-2334 (2003)

T.Hirokawa：“通过毛细管电泳对海水进行痕量离子分析：使用瞬时等速电泳预浓缩测定碘化物”电泳。

T.Hirokawa et al.: "High-sensitive capillary zone electrophoresis analysis by electrokinetic injection ォkith transient isotachophoretic preconcentration Electrokinetic supercharging"Electrophoresis. 24. 498-504 (2003)

T. Hirokawa 等人：“通过电动注射相瞬态等速电泳预浓缩电动增压进行高灵敏度毛细管区带电泳分析” 电泳 24. 498-504 (2003)

J-B.Kim et al.: "Mechanistic Study on Analyte Focusing by Dynamic pH Junction in Capillary Electrophoresis Using Computer Simulation"Anal.Chem.. 75. 3986-3993 (2003)

J-B.Kim 等人：“利用计算机模拟对毛细管电泳中动态 pH 连接分析物聚焦的机制研究”Anal.Chem.. 75. 3986-3993 (2003)