上咽頭癌におけるGalectin-3の機能解析

Galectin-3在鼻咽癌中的功能分析

• 批准号: 14770896
• 负责人: 赤埴 詩朗
• 金额: $ 2.05万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14770896/
• 关键词: 上咽頭癌; Chemo-Radiation; Galectin-3; アポトーシス

1.上咽頭癌及び正常組織におけるGalectin-3の発現の解析当施設の上咽頭癌約50症例が研究対象である。Chemo-Radiation前の上咽頭癌及び周囲正常組織の生検標本を用いてABC法にて顕微鏡下にGalectin-3を同定しまた同時に良性腫瘍や異形成等の前癌病変においても同様にGalectin-3を同定しその比較定量を行なった。正常粘膜ではGalectin-3の発現は抑えられているが上咽頭由来のポリープでは炎症細胞浸潤の程度によりその発現レベルが左右されることが判明した。加えてGalectip-3の発現が正常粘膜、異形成、癌といった一連の過程で正の相関をもって増加することも判明し一定の研究成果があったがGalectin-3の発現の程度と腫瘍分化度等の予後因子ならびに再発、生命予後との関連については症例数を増やして解析中である。2.上咽頭癌の薬剤耐性におけるGalectin-3の関与上咽頭癌細胞株が得られなかったため生検目的で切除した上咽頭癌組織切片から細胞浮遊液を作製した後Single Cell Cultureを行い現在新たな上咽頭癌細胞株を樹立した。樹立後この上咽頭癌細胞株にベクタを用いてGalectin-3のcDNAを形質導入し(以下Transfectantと記す)、このTransfectantのおいてGalectin-3の発現が増大されることを確認し、次にベクタのみを形質導入した対照との間でシスプラチン感受性、シスプラチンによるアポトシス誘導等を細胞生物学的手法を用いて比較検討した。結果Transfectantはシスプラチン抵抗性を持ちシスプラチン処理におけるGalectin-3の抗アポトーシス機能の関与が示唆された。

1. Analysis of Galectin-3 findings in upper pharynx cancer and normal tissues when about 50 cases of upper pharynx cancer were studied. The biomarkers of pre-Chemo-Radiation upper pharynx cancer and normal tissues of peripharynx were detected by ABC method. The Galectin-3 markers were detected by the same assay and the Galectin-3 markers were detected by quantitative analysis. Normal mucous membrane Galectin-3 showed inhibition of pharynx and pharynx, inflammation, cellular infiltration, cell infiltration, inflammation, inflammation, cellular infiltration, inflammation, pharynx, pharynx and pharynx. The following factors, such as normal mucous membrane, formation, cancer, normal mucosa, formation, cancer, Galectin-3, degree of differentiation, and so on, were analyzed in the analysis of the number of cases in which normal mucosa, cancer and cancer were detected. two。 Upper pharynx cancer tolerance Galectin-3 cell lines and upper pharynx cancer cell lines were obtained. objective to remove the cancer tissue section of the upper pharynx and to detect the cell line of the new pharynx cancer cell line by Single Cell Culture after the tissue section of the upper pharynx cancer. After that, the cell lines of upper pharynx cancer were exposed to Galectin-3 cDNA (the following Transfectant records), and the following Transfectant records were used. The results showed that the sensitivity of the tumor was significantly higher than that of the control group (the following Transfectant records). The methods of cell biology, such as the guidance of cell biology, are used to compare the results of cell biology. Results the results of Transfectant test showed that the resistance rate of Galectin-3 was significantly higher than that of control group (P < 0.01).