加齢変化が嗅神経細胞の産生・維持機構に及ぼす影響についての基礎的研究

衰老对嗅觉神经元产生和维持机制影响的基础研究

• 批准号: 14770886
• 负责人: 近藤 健二
• 金额: $ 1.73万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14770886/
• 关键词: 嗅神経細胞; 老化; 再生; 免疫組織化学; olfactory marker protein; BrdU

対象と方法生後10日、3ヶ月、16ヶ月のICRマウスに細胞増殖マーカーであるBrdUを投与し、投与後2時間、1日、2日、3日、5日、7日、14日、28日後に各群3〜5匹ずつ固定、鼻腔組織を摘出した。これを脱灰ののち包埋、冠状断で薄切した。切片を抗BrdU抗体および成熟嗅神経細胞の特異的マーカーである抗OMP抗体を用いて免疫2重染色し、BrdU陽性細胞の分布およびそのBrdU投与後の時間経過による変化、さらに同細胞がOMPと2重に陽性になるまでの時間経過を各年齢群で比較し、嗅神経細胞の前駆細胞の増殖能、およびこれが成熟嗅神経細胞に分化する速度を検討した。結果と考察10日齢群においてBrdU陽性細胞は次のような細胞動態を示した。まずBrdU投与後2時間ではBrdU陽性細胞は嗅上皮の基底部および最表層の支持細胞層に位置していた。投与後1〜3日目では個々のBrdU染色がやや薄く、または核内の小顆粒状になり、陽性細胞数はやや増加した。またBrdU陽性細胞の一部がやや表層に移動したが、明らかなOMPとの2重陽性を示す細胞は確認できなかった。5日目ではOMP染色性を示すBrdU陽性細胞が出現し、7日目ではこの2重陽性細胞の数が増加した。14日目ではほとんど全てのBrdU陽性細胞がOMP陽性となったが、BrdU陽性細胞の総数は7日目に比べ大きく減少した。28日目はさらにBrdU陽性細胞数が減少していた。3ヶ月齢群、16ヶ月齢群においてはBrdU陽性細胞数が10日齢群に比べて大きく減少していたが、OMPとの2重陽性を示す細胞の出現過程については10日齢群とほぼ同様の所見であった。以上の結果よりBrdUを取り込んだ新生細胞がOMP陽性となるまでの時間経過は10日齢群と16ヶ月齢群で大きな差異は認められず、嗅神経への分化速度は加齢変化で大きな影響を受けていないことが推察される。

The ICR test was conducted on the 10th, 3rd, and 16th day after birth, and the BrdU test was conducted on the 2nd, 1st, 2nd, 3rd, 5th, 7th, 14th, and 28th day after birth. This is the first time that a person has ever been involved in a crime. Anti-BrdU antibody and specific OMP staining of mature olfactory neurons were observed in sections. Distribution of BrdU-positive cells and time lapse after BrdU administration were observed. Time lapse after BrdU-positive cells became OMP and 2-positive cells was observed. Population comparisons were made among different years. Proliferation of olfactory neurons and differentiation rate of mature olfactory neurons were observed. Results The dynamic changes of BrdU positive cells in 10-day-old group were observed. 2 hours after BrdU administration, BrdU positive cells were located in the basal part of olfactory epithelium and the uppermost supporting cell layer. 1 ~ 3 days after injection, the number of BrdU positive cells increased, and the number of small granular cells increased. Some BrdU-positive cells were found to be mobile in the surface layer, while others were positive in the surface layer. OMP staining showed that BrdU positive cells appeared on the 5th day, and the number of double positive cells increased on the 7th day. On the 14th day, the total number of BrdU-positive cells decreased compared with that on the 7th day. On the 28th, the number of BrdU positive cells decreased. The number of BrdU-positive cells in the 3-day-old group and the 16-day-old group decreased greatly compared with that in the 10-day-old group, and the number of OMP-positive cells in the 2-day-old group decreased significantly compared with that in the 10-day-old group. The above results indicate that the differentiation rate of newborn cells from OMP positive to OMP positive is significantly different from that of OMP positive cells from 10 days to 16 months, and that the differentiation rate of olfactory cells is significantly different from that of OMP positive cells from 10 days to 16 months.

项目成果

Ishimoto S, Kondo K, Ito K, Oshima K: "Hoarseness after cardiac surgery : possible contribution of low temperature to the recurrent nerve paralysis."Laryngoscope. 113. 1088-1089 (2003)

Ishimoto S、Kondo K、Ito K、Oshima K：“心脏手术后声音嘶哑：低温可能导致复发性神经麻痹。”喉镜。