Dynamic regulation of cell polarity in Arabidopsis

拟南芥细胞极性的动态调控

• 批准号: 5332901
• 负责人: Professor Dr. Klaus Palme
• 金额: --
• 依托单位: Institut für Biologie II
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5332901?language=en
• 关键词: Dynamic; regulation; cell; polarity; Arabidopsis

We are interested in the mechanisms by which plant cells and tissues develop and maintain polarity. To date, there is only very little known about how plant cells establish polarity and whether there is a common mechanism used by different plant cells, between different plant species, or between plants and other eukaryotic organisms. What are the mechanisms for polarity establishment? We recently identified the first polarly localised plasma membrane proteins, members of the PIN gene family, and members of the plant Rho GTPase gene family that serve as markers for cell polarity in plants. We will use these unique markers to genetically dissect the biochemical machinery involved in their polar distribution. Genetic and biochemical information of this machinery will be essential to understand how intracellular asymmetry is generated and maintained. These experiments will also help to assess how cells within tissues use spatial cues to align their axis of symmetry and to direct an asymmetry to one cortical region of a plant cell. In our studies we will address (i) the functional basis of the cellular apical-basal organisation, (ii) the mechanisms through which polar exocytotic activity is regulated, and (iii) the consequences for plant cell and whole plant function of interfering with the exocytotic pathway.

我们对植物细胞和组织发展和维持极性的机制很感兴趣。迄今为止，关于植物细胞如何建立极性，以及不同植物细胞之间、不同植物物种之间或植物与其他真核生物之间是否存在共同的机制，我们所知甚少。极性建立的机制是什么？我们最近发现了第一个极性定位的质膜蛋白，PIN基因家族成员和植物Rho GTPase基因家族成员，它们作为植物细胞极性的标记。我们将使用这些独特的标记来从遗传学上剖析与它们的极性分布有关的生化机制。这种机制的遗传和生化信息对于理解细胞内不对称是如何产生和维持的至关重要。这些实验也将有助于评估组织内的细胞如何利用空间线索来调整它们的对称轴，并将不对称指向植物细胞的一个皮质区域。在我们的研究中，我们将解决(i)细胞顶基组织的功能基础，（ii）通过极性胞外活动调节的机制，以及（iii）干扰胞外通路对植物细胞和整个植物功能的影响。