Molecular and functional profiling of vesicle trafficking pathways in Arabidopsis thaliana

拟南芥囊泡运输途径的分子和功能分析

• 批准号: 5402544
• 负责人: Professor Dr. Klaus Palme
• 金额: --
• 依托单位: Institut für Biologie II
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5402544?language=en
• 关键词: Molecular; functional; profiling; vesicle; trafficking

The secretory pathway of plants controls homeostasis of cellular membrane compartments, and transports macromolecules between intracellular compartments and the extracellular space. Despite conservation of the basic secretory machinery between plants and other eukaryotes, recent findings suggest distinct structural and functional differences in plants. To systematically study key players of plant secretion, which control formation of transport vesicles, vesicle delivery to target membranes, and intracellular trafficking by internal and external cues, we propose a combined functional genomics and proteomics approach. Focus will be on the Rab and ARF small G protein families and their cellular partners from Arabidopsis thaliana. Such an approach will uncover important aspects of the organisational blue print of the plant secretory pathway, its fundamental functional differences to other eukaryotes, and, finally, its contribution to form and function of plants.

植物的分泌途径控制细胞膜区室的稳态，并在细胞内区室和细胞外空间之间运输大分子。尽管植物和其他真核生物之间的基本分泌机制的保护，最近的研究结果表明，在植物中的结构和功能的差异。为了系统地研究植物分泌的关键参与者，这些参与者控制运输囊泡的形成，囊泡递送到靶膜，以及通过内部和外部线索的细胞内运输，我们提出了功能基因组学和蛋白质组学相结合的方法。重点将放在Rab和ARF小G蛋白家族和他们的细胞合作伙伴从拟南芥。这种方法将揭示植物分泌途径的组织蓝图的重要方面，其与其他真核生物的基本功能差异，以及最后，其对植物的形式和功能的贡献。