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Regulation of plant development by known and unknown inter-cellular signaling molecules

已知和未知的细胞间信号分子对植物发育的调节

基本信息

批准号：
15107001
负责人：
KAKIMOTO Tatsuo
金额：
$ 67.97万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (S)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2007
项目状态：
已结题

项目摘要

The aim of this study is to understand how inter-cellular signaling molecules regulate plant development. This study focused on biosynthesis, perception, signal transduction, and biological role of a classical plant hormone, cytokinin; and identification of new signaling molecules. Before the start of this project, we identified biosynthetic enzymes and a receptor of cytokinins, however, detailed analysis was yet to be done. In this project, we have clarified the biosynthetic routes of cytokinins, using multiple mutants of cytokinin biosynthetic isopentenyltransferases and tRNA modifying isopentenyltransferases. The multiple mutant also revealed the functions of endogenous cytokinins, including a novel function, regulation of cambial activity. We also disrupted all cytokinin receptor genes, which provided evidence that cytokinins are perceived exclusively by CRE1-class histidine kinases. We explored into detailed mechanisms of cytokinin signal transduction, showing that cytokinins regulate bidirectional phosphorelay signaling. We also screened for novel intercellular signaling molecules. For the first screening we overexpressed genes coding for putative secretory small products, and we found a number of genes that cause interesting phenotypes. Among those genes, we examined the roles of two genes, for which we named EPF1 and EPF2. EPF1 regulates the pattern of stomata, and EPF2 regulates epidermal cell density. Although the action-timings of EPF1 and EPF2 differ, they are perceived and transduced by the same set of proteins, TMM receptor-like protein, ER-family receptor kinases, and a MAPKKK, YODA. We will clarify the function of other candidate signaling-molecules.

本研究的目的是了解细胞间信号分子如何调控植物发育。本研究的重点是经典植物激素细胞分裂素的生物合成、感知、信号传导和生物学作用;以及新信号分子的鉴定。在此项目开始之前，我们确定了生物合成酶和细胞分裂素受体，但尚未进行详细分析。在这个项目中，我们已经澄清了细胞分裂素的生物合成途径，使用细胞分裂素生物合成异戊烯基转移酶和tRNA修饰异戊烯基转移酶的多个突变体。多重突变体还揭示了内源细胞分裂素的功能，包括一个新的功能，形成层活动的调节。我们还破坏了所有的细胞分裂素受体基因，这提供了细胞分裂素只被CRE 1类组氨酸激酶感知的证据。我们探讨了细胞分裂素信号转导的详细机制，表明细胞分裂素调节双向磷酸化信号转导。我们还筛选了新的细胞间信号分子。对于第一次筛选，我们过表达编码推定的分泌小产物的基因，我们发现了一些引起有趣的表型的基因。在这些基因中，我们研究了两个基因的作用，我们将其命名为EPF 1和EPF 2。EPF 1调节气孔的模式，EPF 2调节表皮细胞密度。虽然EPF 1和EPF 2的作用时间不同，但它们被同一组蛋白质、TMM受体样蛋白、ER家族受体激酶和MAPKKK、YODA感知和转导。我们将阐明其他候选信号分子的功能。

项目成果

期刊论文数量（55）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Roles of Arabidopsis ATP/ADP isopenteuyltransferases and tRNA isopentenyltransferases in cytokinin biosyathesis.

拟南芥 ATP/ADP 异戊烯基转移酶和 tRNA 异戊烯基转移酶在细胞分裂素生物合成中的作用。

DOI：
发表时间：
2006
期刊：
Proc Natl Acad Scs USA 103
影响因子：
0
作者：
Miyawaki K;Tarkowski P;Matsumoto-Kitano M;Kato T;Sato S;Tarkowska D;Tabata S;Saudberg G;Kakimoto T.
通讯作者：
Kakimoto T.

Roles of Arabidopsis ATP/ADP isopentenyltransferases and tRNA isopentenyltransferases in cytokinin biosynthesis

DOI：
10.1073/pnas.0603522103
发表时间：
2006-10-31
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Miyawaki, Kaori;Tarkowski, Petr;Kakimoto, Tatsuo
通讯作者：
Kakimoto, Tatsuo

AHK5 histidine kinase regulates root elongation through an ETR1-dependent abscisic acid and ethylene signaling pathway in Arabidopsis thaliana