Nuclear inclusions, neurodegeneration and related molecules-tridimensional study

1.用催化报告细胞沉积法扩增一个或两个信号，实现三重免疫荧光标记（J Histochem Cytochem 2003:51:12 1-6, J Neurosci Methods 2004:135:67-70）。这使得超灵敏的信号检测与非扩增信号区分，即使这两个信号是由来自同一物种的抗体探测的。在SCA1脑的脑桥神经元上，定量测定含有核包裹体（NIs）的核的交易面积，并与不含NIs的神经元进行比较。更大的交易区与NI的存在相关，表明NI与神经保护有关，而不是神经元死亡。[J] .中华神经外科杂志，2003,24:344 - 344。一个古老的尸检病例皮埃尔·玛丽小脑病变重新评估，发现与Machado-Joseph病无法区分（Arch Neurol 2004:61:784-790）4。脑梗死或炎症过程中的Tau沉积被发现与退行性Tau病有区别，因为没有磷酸化和原纤维结构。用Gallyas银法发现4-repeat tau沉积与嗜银症相关（Acta Neuropathol 2005:109:299-305, ibid 2005:110:158-164, J Neurol Sci in press），用Campbell法发现3-repeat tau沉积与嗜银症相关（Acta Neuropathol 2005:109:483-489, Handbook of Clinical Neurol in press）

1.Triple-immunofluorolabeling was achieved through amplification of one or two of the signals with catalyzed reporter deposition method (J Histochem Cytochem 2003:51:1201-6, J Neurosci Methods 2004:135:67-70). This allowed supersensitive detection of the signals distinguishable from non amplified signals even if the two signals were probed with antibodies from the same species.2.On pontine neurons of SCA1 brains, transactional area of nuclei containing nuclear inclusions (NIs) was quantified and was compared with that of neurons not containing NIs. Larger transactional area was correlated with the presence of NI, suggesting that NI is related to neuroprotection rather than neuronal death. (J Neurol Neurosurg Psychiatry 2003:74:597-601, Adv Neurol Res 2004:48:334-345)3.An old autopsy case of Heredoataxie cerebelleuse de Pierre Marie was reevaluated and found to be indistinguishable from Machado-Joseph disease (Arch Neurol 2004:61:784-790)4.Tau deposition in cerebral infarction or inflammatory process was found to be distinguishable from degenerative tauopathy in terms of the absence of phosphorylation and fibrillary structure.5.Deposit of 4-repeat tau was found to be correlated with argyrophilia with Gallyas silver method (Acta Neuropathol 2005:109:299-305, ibid 2005:110:158-164, J Neurol Sci in press) and that of 3-repeat tau with Campbell method (Acta Neuropathol 2005:109:483-489, Handbook of Clinical Neurol in press)