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Nuclear inclusions, neurodegeneration and related molecules-tridimensional study

核内含物、神经变性及相关分子-三维研究

基本信息

批准号：
15300118
负责人：
UCHIHARA Toshiki
金额：
$ 4.22万
依托单位：
Tokyo Metropolitan Organization for Medical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300118/
关键词：
CAG repeat disease nuclear inclusion multifluorolabeling three dimensional reconstruction silver staining tauopathy シヌクレイノパチー SCA1 Catalyzed reporter deposition

项目摘要

1.Triple-immunofluorolabeling was achieved through amplification of one or two of the signals with catalyzed reporter deposition method (J Histochem Cytochem 2003:51:1201-6, J Neurosci Methods 2004:135:67-70). This allowed supersensitive detection of the signals distinguishable from non amplified signals even if the two signals were probed with antibodies from the same species.2.On pontine neurons of SCA1 brains, transactional area of nuclei containing nuclear inclusions (NIs) was quantified and was compared with that of neurons not containing NIs. Larger transactional area was correlated with the presence of NI, suggesting that NI is related to neuroprotection rather than neuronal death. (J Neurol Neurosurg Psychiatry 2003:74:597-601, Adv Neurol Res 2004:48:334-345)3.An old autopsy case of Heredoataxie cerebelleuse de Pierre Marie was reevaluated and found to be indistinguishable from Machado-Joseph disease (Arch Neurol 2004:61:784-790)4.Tau deposition in cerebral infarction or inflammatory process was found to be distinguishable from degenerative tauopathy in terms of the absence of phosphorylation and fibrillary structure.5.Deposit of 4-repeat tau was found to be correlated with argyrophilia with Gallyas silver method (Acta Neuropathol 2005:109:299-305, ibid 2005:110:158-164, J Neurol Sci in press) and that of 3-repeat tau with Campbell method (Acta Neuropathol 2005:109:483-489, Handbook of Clinical Neurol in press)

1.三重免疫荧光标记是通过催化报告沉淀法扩增一个或两个信号而实现的(《组织化学细胞化学杂志》2003：51：1201-6，《神经科学杂志》方法2004：135：67-70)。这使得能够超灵敏地检测到与非放大信号区分开来的信号，即使这两个信号是用来自同一物种的抗体探测的。2.在SCA1脑的脑桥神经元上，含有核包涵体(NIS)的核团的交易面积被量化，并与不含NIS的神经元的交易面积进行比较。更大的交易面积与NI的存在相关，表明NI与神经保护有关，而不是与神经元死亡有关。《神经外科精神病学杂志》2003：74：597-601，ADV Neurol Res 2004：48：334-345)3.对一例陈旧性Heredoataxie erebelleusde Pierre Marie尸检病例进行重新评估，发现其与马查多-约瑟夫病(Arch Neurol 2004：61：784-790)无法区分。4.脑梗塞或炎症过程中Tau的沉积与退行性变的区别在于缺乏磷酸化和纤维结构。5.用Galyas银法发现4-重复tau的沉积与嗜银细胞增多有关(中华神经病学报2005：109：299-305,2005：110：158-164J Neurol Sci in Press)和3-Repeat tau的Campbell方法(神经病理学学报2005：109：483-489，《临床神经学手册》出版社)