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Stock identification of cultured fish and shellfish by composition of trace elements in the hard tissues : a new technology for identification of product origin.

通过硬组织中微量元素的成分来鉴定养殖鱼类和贝类的库存：一种识别产品来源的新技术。

基本信息

批准号：
15380125
负责人：
TAKAGI Yasuaki
金额：
$ 6.53万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

For trace-elemental fingerprint studies in fish otoliths, industry-coupled plasma mass spectrography (ICPMS) has not used in Japan. The present study was undertaken to develop ICPMS-based trace-elemental fingerprint studies in the hard tissues of cultured fish and shellfish in Japan. Following results were obtained.1. We developed standard methods to determine trace-elemental composition of fish otoliths and shells of shellfish using solution-based and laser abration ICPMS. Trace-elemental compositions were useful fingerprints that could identify product origin of cultured species.2. We clarified migratory patterns of some salmonid and eel species using Sr contents and Sr/Ca ratio of otoliths determined by EPMA and revealed that the method is a powerful tool to study migration patterns of anadromous, catadromous or diadromous fishes.3. We developed a DNA microarray to identify stocks of chum salmon. The array was accurate and time effective, and required no special equipments such as DNA sequencer. Therefore, the method is especially a powerful tool to identify chum salmon stocks in the field study and on board.4. We immunohistochemically localized otolith matrix proteins, OMP-1 and otolin-1, and suggested important rolls of these proteins in calcification and formation of otolith micro structures. We also revealed that the aragonite saturation ratio of endolymph was maintained over 1.0 through the day-night cycle, suggesting aragonite crystal growth could be continued through a day. On the other hand, mRNA expression of otolin-1 showed clear daily rhythm with significantly higher expressions during night-time, suggesting a key fuction of otolin-1 to construct daily rings. In masu salmon, increases in Sr concentrations and Sr/Ca ratios in the serum occurred 7 days after the seawater transfer. Such a time-lag must be taken into account to reconstruct migration patterns in the anadromous fishes.

对于鱼类耳石中痕量元素指纹研究，日本尚未使用工业耦合等离子体质谱（ICPMS）。本研究进行了开发ICPMS为基础的痕量元素指纹研究在日本的养殖鱼类和贝类的硬组织。主要研究结果如下：1.我们开发了标准的方法来确定微量元素的组成，鱼类耳石和贝类壳使用溶液为基础的和激光磨蚀ICPMS。微量元素成分是鉴别养殖品种产品来源的有用指纹图谱.利用电子探针测定的耳石Sr含量和Sr/Ca比值，阐明了部分鲑科鱼类和鳗鱼的洄游模式，揭示了该方法是研究溯河、降河或溯河洄游鱼类洄游模式的有力工具.我们开发了一种DNA微阵列来识别鲑鱼种群。该芯片准确、快速，不需要DNA测序仪等特殊设备。因此，该方法特别是一个强有力的工具，以识别鲑鱼种群在现场研究和船上.我们的电子化学定位的耳石基质蛋白，OMP-1和otolin-1，并建议这些蛋白质的钙化和形成的耳石微结构的重要辊。我们还发现，文石饱和比的内淋巴保持在1.0以上，通过昼夜循环，这表明文石晶体的生长可以持续一天。另一方面，otolin-1的mRNA表达呈现明显的日节律，夜间表达量显著增加，提示otolin-1在构建日年轮中具有重要作用。在马苏鲑鱼，增加血清中的Sr浓度和Sr/Ca比发生7天后，海水转移。在重建溯河产卵鱼类的洄游模式时，必须考虑到这种时滞。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Migratory histories of three types of Cottus pollux (small-egg, middle-egg, and large-egg types), as revealed by otolith microchemistry.

耳石微化学揭示了三种 Cottus pollux（小卵型、中卵型和大卵型）的迁徙历史。

DOI：
发表时间：
2003
期刊：
Ichthyological Research 50
影响因子：
0
作者：
Goto;A.et al.
通讯作者：
A.et al.

Occurrence of the non-anadromous life history in the shirauo, Salangichthys microdon

白鱼非溯河产卵生活史的发生

DOI：
发表时间：
2004
期刊：
Journal of Apply Ichthyology 20
影响因子：
0
作者：
Takahashi H.;T Nagai;A.Goto;Koga Kazuto;Takahashi Hiroshi;Yamamoto Yusuke;Shimizu Takaaki;Arai Takaomi
通讯作者：
Arai Takaomi

Small anadromous dolly varden on the southern limit of its distribution, as revealed by otolith Sr:Ca ratios.

耳石 Sr:Ca 比率显示，小型溯河产卵的 dolly varden 位于其分布的南端。