Is there gonadal stimulation hormone in sea urchins?

海胆中是否含有性腺刺激激素？

• 批准号: 23658152
• 负责人: TAKAGI Yasuaki
• 金额: $ 2.41万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658152/
• 关键词: ウニ; 生殖巣; 生殖腺刺激ホルモン; 器官培養; 生体外器官培養; 内分泌; MYP; リアルタイムPCR; ホルモン; エゾバフンウニ; 卵巣; 精巣; 遺伝子微量定量系; 栄養細胞

Sea urchin gonadal growth is characterized by intragonadal nutrient storage and the use of the stored nutrient for gametogenesis. Before gametogenesis initiation, gonads increase in size by accumulating nutrients such as proteins, lipids and carbohydrates in nutritive phagocytes that fill the gonadal lumina of both sexes. The major protein is termed major yolk glycoprotein or major yolk protein (MYP), which is a glycoprotein having molecular weight of 160-180 kDa, and has significant roles in gametogenesis. However, we have no information about gonadal development mechanisms, as well as the regulation mechanism on the synthesis of MYP in gonads. Therefore, to understand the mechanisms of gonadal development, we developed quantitative PCR system of MYP mRNA expression as well as an organ culture systems using sea urchin gonads. These techniques will enable a endocrinological research on gonadal developments in the sea urchin.

海胆性腺生长的特点是通过性腺内的营养储存和使用储存的营养配子发生。在配子发生开始之前，性腺通过在充满两性性腺腔的营养性吞噬细胞中积累营养物质如蛋白质、脂质和碳水化合物来增加大小。主要蛋白质被称为主要卵黄糖蛋白或主要卵黄蛋白（MYP），其是分子量为160-180 kDa的糖蛋白，并且在配子发生中具有重要作用。然而，我们还没有关于性腺发育机制的信息，以及性腺中MYP合成的调控机制。因此，为了了解性腺发育的机制，我们建立了MYP mRNA表达的定量PCR体系以及利用海胆性腺的器官培养系统。这些技术将使内分泌研究的性腺发育的海胆。