Evaluation of cardiac function and anatomy of a knockout mouse with cardiac failure

心力衰竭敲除小鼠的心功能和解剖学评价

• 批准号: 15390385
• 负责人: NISHIDA Hiroshi
• 金额: $ 9.66万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390385/
• 关键词: echo; mouse; heart failure; anatomical; VSD; heart function; 先天性心疾患; ラット; 非侵襲的検査; ノックアウトマウス; Notchシグナル; 心室中隔欠損; 超音波検査; 右心系

The majority of knockout mouse models with failure in cardiovascular system are fatal, since it is difficult for these mice to survive. And, animals without blood vessels cannot live. In the this knockout mouse model that we used, malformation of the heart was localized and limited to the right side, therefore the animals were able to survive and reproduce. The heart was evaluated serially by the noninvasive method of echo and less invasive method of catheter. Prominent hypertrophy in the right atrium and right ventricular was observed in one mouse that died of heart failure. Observation of the mice from day 5 after birth to adulthood was performed using an echo diagnostic system HDI-5000 Sono CT XRES manufactured by PMS Company, using a high frequency linear probe CL15-7. The physical relationship of the blood vessels and the ventricles was evaluated three-dimensionally. Also, the right and left coronary arteries were observed to arise from the aorta. To explore cardiac anatomy and physiological function of a knockout mouse is helpful to demonstrate the intrinsic function of the gene, to identify the new function of the gene from the unexpected phenotype, and to examine the role of the gene in the phenotype by a relation between the genetic background and the genealogical standpoint.

心血管系统衰竭的基因敲除小鼠模型大多数是致命的，因为这些小鼠很难存活。没有血管的动物无法生存。在我们使用的这种基因敲除小鼠模型中，心脏畸形是局部的，并局限于右侧，因此动物能够生存和繁殖。采用超声无创法和导管微创法对心脏进行动态评价。在一只死于心力衰竭的小鼠中观察到右心房和右心室显著肥大。使用PMS公司制造的回波诊断系统HDI-5000 Sono CT XRES，使用高频线性探头CL 15 -7，对小鼠从出生后第5天到成年进行观察。血管和心室的物理关系进行了三维评价。此外，观察到右冠状动脉和左冠状动脉起源于主动脉。研究基因敲除小鼠的心脏解剖结构和生理功能，有助于揭示基因的内在功能，从意外表型中发现基因的新功能，并从遗传背景和系谱学角度探讨基因在表型中的作用。

项目成果

【循環器診療の質を問い直す】循環器診療の質の向上とそれを阻害するもの 循環器診療機関の配置は適正か 心臓血管外科

【重新审视心血管护理质量】心血管护理质量的提高及阻碍因素 心血管护理机构布局是否合适？

• 发表时间: 2005
• 期刊: Cardiovascular Med-Surg 7
• 作者: Nishida H;et al.;西田博;西田博;西田博;西田博
• 通讯作者: 西田博

循環器分野.バイオマテリアル

心血管领域.生物材料

• 发表时间: 2004
• 期刊: 生体材料 22
• 作者: Goya N;Ishikawa N;Ito F;Kobayashi C;Tomizawa Y;Toma H.;西田 博
• 通讯作者: 西田 博

【人工臓器 最近の進歩】 ペースメーカ

[人造器官的最新进展] 起搏器

• 发表时间: 2004
• 期刊: 人工臓器 33
• 作者: 宮川;富田幸子;冨澤康子;他;西田博
• 通讯作者: 西田博

Bilateral versus unilateral internal mammary revascularization in patients with diabetes

• DOI: 10.1161/01.cir.0000085995.87982.6e
• 发表时间: 2003-09-16
• 期刊: CIRCULATION
• 影响因子: 37.8
• 作者: Endo, M;Tomizawa, Y;Nishida, H
• 通讯作者: Nishida, H

Effect of ulinastatin on microcirculation during excessive hemorrhage using fluid therapy.

乌司他丁对液体治疗过度出血期间微循环的影响。

• 发表时间: 2003
• 期刊: In Vivo 17
• 作者: Takada K;Komori M;Notoya A;Tomizawa Y;Ozaki M.
• 通讯作者: Ozaki M.