Research of the change of the construction and biocompatibility of expanded polytetrafluoroethylene (ePTFE) graft surface modified with fibronectin and fibronectin fragments

纤连蛋白及纤连蛋白片段修饰膨体聚四氟乙烯（ePTFE）移植物表面结构及生物相容性变化研究

• 批准号: 15390406
• 负责人: YASUDA Keishu
• 金额: $ 3.84万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390406/
• 关键词: e PTFE; fibronectin fragment; atmospheric pressure glow discharge; carboxylization; 人工血管; フィブロネクチン・フラグメント; 内皮化促進; atomospheric pressure glow discharge

This research was started to examine the endothelialization effect of the ePTFE prosthetic graft whose surface is modified by uniting fibronectin or fibronectin fragment GRGDSP To unite these peptide or protein, the surface of the ePTFE graft should be polymerized with carboxyl substances.At first, we investigate the technique of polymerization of the surface of ePTFE graft. Atmospheric pressure glow (APG) discharge treatment was successfully applied to modify ePTFE grafts from being hydrophobic to being hydrophilic with acryl acid (AAc) or poly acryl acid (PAA). Polymerization of AAc or PAA began occurring on the surface of ePTFE and then continued within porous structures. The surfaces of ePTFE were more effectively modified by PAA than AAc. The outer surface was more hydrophilic than the inner surface. When the outer tube of ePTFE was wrapped with PTFE film and processed using the same experimental method, a markedly enhanced surface modification on the inner surface, especially with the use of AAc, was observed..However, connection of fibronectin fragment to the surface of modified ePTFE grafts was not successful. We could not reach the reason why this process failed.

本研究旨在探讨通过纤连蛋白或纤连蛋白片段GRGDSP结合表面修饰的ePTFE假体移植物的内皮化效果。为了将这些肽或蛋白质结合起来，需要在ePTFE移植物的表面与羧基物质进行聚合。首先，我们研究了ePTFE移植物表面的聚合技术。常压辉光（APG）放电处理已成功应用于用丙烯酸（AAc）或聚丙烯酸（PAA）将ePTFE接枝物从疏水性改性为亲水性。 AAc 或 PAA 的聚合开始发生在 ePTFE 表面，然后在多孔结构内继续进行。 PAA 比 AAc 对 ePTFE 的表面进行了更有效的改性。外表面比内表面更亲水。当ePTFE外管包裹PTFE薄膜并使用相同的实验方法进行加工时，观察到内表面的表面改性显着增强，尤其是使用AAc时。然而，纤连蛋白片段与改性ePTFE移植物表面的连接并不成功。我们无法找到此过程失败的原因。