Analysis of molecular mechanisms of viral growth and immune reaction in ocular herpetic infections

眼部疱疹感染病毒生长和免疫反应的分子机制分析

基本信息

  • 批准号:
    15390531
  • 负责人:
  • 金额:
    $ 7.81万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2006
  • 项目状态:
    已结题

项目摘要

1. Pathpgenesis of herpetic stromal keratitis in CCR5- and CXCR3-deficient mice.We have analyzed two chemokine receptors, CCR5 and CXCR3, which are expressed on CD4+ Th1 cells, in mice herpetic stromal keratitis (HSK) model. CCR5-deficient (CCR5KO), CXCR3-deficient (CXCR3KO), CCR5/CXCR3 double-deficient (DKO) and wild type (WT) mice were infected intracorneally with HSV-1 and examined clinically by biomicroscope. HSK clinical scores in DKO mice were significantly decreased compared with WT mice, which was reversed by the transfer of spleen cells of WT mice. Histologically, corneal infiltrating cells, including T cells (CD4+ and CD8+ cells) and neutrophils were significantly reduced in CCR5KO, CXCR3KO, and DKO mice. Transcript levels of immune-related cell surface marker in the eye were reduced in DKO mice. There were no significant differences of virus titers in eyes among any groups of mice. Collectively, the suppression of chemotaxis and activation of CD4+ Th1 cells by the lacking of … More CXCR3 and CCR5 caused the decrease of other infiltrating cells, resulting in the reduction of HSK without any increase of viral growth.2. Clinical application of real-time polymerase chain reaction for diagnosis of herpetic diseases of anterior segment of the eyeTo assess the value of quantification of herpes simplex virus (HSV)-DNA for the differential diagnosis of herpetic diseases of the anterior segment of the eye, 144 samples of 90 patients with ocular inflammatory diseases were examined for HSV-DNA by real-time PCR. In cases of typical herpetic epithelial keratitis, a high number of copies of HSV-DNA were detected (mean : 1.0×10^7 copies in epithelial scrapings and 3.5×10^5 copies in tear fluid). In stromal keratitis, the copies of HSV-DNA in the tear fluid (mean : 4.7×10^2 copies) was significantly lower than that in cases of epithelial keratitis. In the cases tested to exclude HSV, 17.0% showed low copy numbers of HSV-DNA indicating an unexpected involvement of HSV in these cases. Collectively, real-time PCR is an informative method to diagnose herpetic eye diseases and evaluate the possible involvement of HSV in other inflammatory ocular diseases. Less
1. CCR 5和CXCR 3缺陷小鼠疱疹性角膜基质炎的发病机制我们分析了两种趋化因子受体CCR 5和CXCR 3在小鼠疱疹性角膜基质炎(HSK)模型中的表达。用HSV-1角膜内感染CCR 5缺陷型(CCR 5 KO)、CXCR 3缺陷型(CXCR 3 KO)、CCR 5/CXCR 3双缺陷型(DKO)和野生型(WT)小鼠,并通过生物显微镜进行临床检查。与WT小鼠相比,DKO小鼠的HSK临床评分显著降低,而WT小鼠的脾细胞转移可逆转这种情况。在组织学上,CCR 5 KO、CXCR 3 KO和DKO小鼠中角膜浸润细胞,包括T细胞(CD 4+和CD 8+细胞)和中性粒细胞显著减少。在DKO小鼠中,眼睛中免疫相关细胞表面标志物的转录水平降低。各组小鼠眼部病毒滴度无显著性差异。总的来说,缺乏CD 4 + Th 1细胞对趋化性和活化的抑制 ...更多信息 CXCR 3和CCR 5引起其他浸润细胞的减少,导致HSK的减少而不增加病毒的生长.实时荧光定量聚合酶链反应(real-time PCR)检测单纯疱疹病毒(HSV)-DNA在眼前段疱疹性疾病中的临床应用为探讨HSV-DNA定量检测在眼前段疱疹性疾病鉴别诊断中的价值,采用实时荧光定量PCR方法检测了90例眼部炎症性疾病患者144份标本中的HSV-DNA。在典型的疱疹性角膜上皮炎病例中,检测到大量的HSV-DNA拷贝(平均:上皮刮片中为1.0×10^7拷贝,泪液中为3.5×10^5拷贝)。基质性角膜炎泪液中HSV-DNA的拷贝数(平均4.7×10^2拷贝)明显低于上皮性角膜炎。在排除HSV的检测病例中,17.0%显示HSV-DNA的低拷贝数,表明这些病例中存在HSV的意外参与。总的来说,实时PCR是一种诊断疱疹性眼病和评估HSV可能参与其他炎症性眼病的信息方法。少

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Strategy against ocular surface infections
对抗眼表感染的策略
角膜ヘルペスが難治になる時
当角膜疱疹变得顽固时
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Zhang W et al.;井上幸次;Inoue Y;井上幸次;井上幸次;Inoue Y;Inoue Y;井上 幸次;Inoue Y;井上 幸次;井上幸次;Inoue Y;井上 幸次;Kosaki R et al.;三浦和美他;井上幸次;井上幸次
  • 通讯作者:
    井上幸次
Varicella Keratitis
水痘角膜炎
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Zhang W et al.;井上幸次;Inoue Y;井上幸次;井上幸次;Inoue Y;Inoue Y;井上 幸次;Inoue Y;井上 幸次;井上幸次
  • 通讯作者:
    井上幸次
井上幸次: "アトピー性皮膚炎と眼感染症"臨床眼科. 57. S40-S45 (2003)
Koji Inoue:“特应性皮炎和眼部感染”临床眼科 57. S40-S45 (2003)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Dendritic keratitis caused by an acyclovir-resistant herpes simplex virus with frameshift mutation.
由具有移码突变的抗阿昔洛韦单纯疱疹病毒引起的树突状角膜炎。
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    W.Zhang;T.Suzuki;A.Shiraishi;I.Shimamura;Y.Inoue;Y.Ohashi
  • 通讯作者:
    Y.Ohashi
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INOUE Yoshitsugu其他文献

INOUE Yoshitsugu的其他文献

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{{ truncateString('INOUE Yoshitsugu', 18)}}的其他基金

Molecular analysis for anti-inflammatory treatment against infectious keratitis
感染性角膜炎抗炎治疗的分子分析
  • 批准号:
    17K11481
  • 财政年份:
    2017
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analyses of molecular target in the treatment of herpetic keratitis
带状疱疹性角膜炎治疗的分子靶点分析
  • 批准号:
    20592076
  • 财政年份:
    2008
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Immunological Control of Ocular Herpetic Infections
眼部疱疹感染的免疫控制
  • 批准号:
    12470365
  • 财政年份:
    2000
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The Roles of T Cells in the Pathogenesis of Ocular Viral Infections
T 细胞在眼部病毒感染发病机制中的作用
  • 批准号:
    09470379
  • 财政年份:
    1997
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

Corneal neuropeptides and herpetic stromal keratitis
角膜神经肽与疱疹性基质角膜炎
  • 批准号:
    8616376
  • 财政年份:
    2013
  • 资助金额:
    $ 7.81万
  • 项目类别:
Corneal neuropeptides and herpetic stromal keratitis
角膜神经肽与疱疹性基质角膜炎
  • 批准号:
    8504248
  • 财政年份:
    2013
  • 资助金额:
    $ 7.81万
  • 项目类别:
Corneal neuropeptides and herpetic stromal keratitis
角膜神经肽与疱疹性基质角膜炎
  • 批准号:
    9248405
  • 财政年份:
    2013
  • 资助金额:
    $ 7.81万
  • 项目类别:
Corneal neuropeptides and herpetic stromal keratitis
角膜神经肽与疱疹性基质角膜炎
  • 批准号:
    8912631
  • 财政年份:
    2013
  • 资助金额:
    $ 7.81万
  • 项目类别:
Corneal neuropeptides and herpetic stromal keratitis
角膜神经肽与疱疹性基质角膜炎
  • 批准号:
    9034583
  • 财政年份:
    2013
  • 资助金额:
    $ 7.81万
  • 项目类别:
Corneal neuropeptides and herpetic stromal keratitis
角膜神经肽与疱疹性基质角膜炎
  • 批准号:
    8812856
  • 财政年份:
    2013
  • 资助金额:
    $ 7.81万
  • 项目类别:
Role of corneal neuropeptides in the pathogenesis of herpetic stromal keratitis
角膜神经肽在疱疹性基质性角膜炎发病机制中的作用
  • 批准号:
    7875060
  • 财政年份:
    2010
  • 资助金额:
    $ 7.81万
  • 项目类别:
Role of corneal neuropeptides in the pathogenesis of herpetic stromal keratitis
角膜神经肽在疱疹性基质性角膜炎发病机制中的作用
  • 批准号:
    8035311
  • 财政年份:
    2010
  • 资助金额:
    $ 7.81万
  • 项目类别:
A novel peptide therapy for treatment of herpetic stromal keratitis
一种治疗疱疹性基质性角膜炎的新型肽疗法
  • 批准号:
    7512143
  • 财政年份:
    2009
  • 资助金额:
    $ 7.81万
  • 项目类别:
A novel peptide therapy for treatment of herpetic stromal keratitis
一种治疗疱疹性基质性角膜炎的新型肽疗法
  • 批准号:
    7945287
  • 财政年份:
    2009
  • 资助金额:
    $ 7.81万
  • 项目类别:
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