The establishment of methods for reproduction of functional teeth by use of long-term culture

建立利用长期培养繁殖功能性牙齿的方法

• 批准号: 15390553
• 负责人: IMAI Hajime
• 金额: $ 9.28万
• 依托单位: Showa University (2004-2006)Tokyo Medical and Dental University (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390553/
• 关键词: whole embryo culture (WEC); the long-term maxillofacial organ culture; tooth-reproduction; anterior axial mesendoderm; prechordal plate; anterior neural ridge (ANR); Rathke's pouch; pituitary mormone; 頭部中内胚葉; 歯; 腺性下垂体; 再生法; ラット全胚培養法; 上顔面器官培養法

The purposes 1 and 2 of this study was "making an index about multipotent-rerated genes in cranial neural crest cells (NCCs) by the elucidating gene expression during tooth development" and "establishing a method to produce multipotenial cells by use of the index mentioned above", respectively. However, the amount of RNA of NCCs in the histological section was too small to make the index mentioned above. Therefore, I had stopped the experiments. Therefore, we mainly carried out the studies for "establishing methods reproducing the tooth, periodontal tissues and other craniofacial organs by use of rat whole embryo culture (WEC)", i.e., the purposes 3.During above experimens, we focused on the differences between developmental processes of tooth and pituitary, and carried out the studies. We got new findings as follows. 1. The ectoderm-endoderm boundaries and midbrain-hindbrain NCCs contributes to tooth initiation. 2. The non-neuronal anterior neural ridge (ANR) of ectoderm-endoderm boundaries gives rise to ACTH-producing cells. 3. The prechordal plate (PrP) intrudes itself in ANR and neural plate, contributes to prechordal mesenchyme and intraforebrain mesenchyme, and gives rise to endocrine cells in pituitary. 4. The site-specific distribution of PrP-derived cells is required to the differentiation of the tooth, adenohypophysis and hypothalamus. 5. The many developmental defects occur in PrP-ablation mice similarly to in Shh K.O. mice. We established the niche for reproducing the cells producing pituitary hormones, e.g., ACTH,αGSU,αMsh, TSHβ and LHβ, in the above results 3 by the long-term maxillofacial organ culture of Rathck's pouch accompanied with diencephalon. 6. We show the domain that is important for reproduction of tooth, jaw in a development process of a chin of birds. 7. We are establishing the reproduction methods to craniofacial organ, including hypothalamic nucleus relating to feeding behavior, by use of WEC.

这项研究的目的1和2是“通过在牙齿发育过程中阐明基因表达的颅神经元细胞（NCC）中的多能量基因的指数”，并“建立一种通过上述指数分别使用上述索引来产生多局部细胞的方法”。但是，组织学部分中NCC的RNA量太小，无法使上面提到的索引。因此，我停止了实验。因此，我们主要进行了“建立牙齿，牙周组织和其他颅面器官的方法，通过使用大鼠整个胚胎培养（WEC）（即目的）3。在上述实验中，我们专注于牙齿和垂体发展过程之间的差异，以及研究。我们得到的新发现如下。 1。外胚层 - 内胚层边界和中脑 - 脑NCCS有助于牙齿启动。 2。非胚层 - 胚层边界的非神经前神经元脊（ANR）产生了产生ACTH的细胞。 3。预教板（PRP）在ANR和神经元板中侵入自身，有助于前间充质和外脑内间质，并在垂体中引起内分泌细胞。 4。牙齿分化，腺旋脑分析和下丘脑的分化需要特定于PRP衍生细胞的位点特异性分布。 5。许多发育缺陷发生在PRP燃烧小鼠中，与SHH K.O中的相似。老鼠。我们在上述结果中建立了产生垂体激素的细胞，例如Acth，αGSU，αMSH，TSHβ和LHβ，在上述结果中，由Rathck袋的长期颌面器官培养3。 6。我们显示了在鸟下巴的发育过程中繁殖牙齿，下巴很重要的域。 7。我们正在建立对颅面器官的繁殖方法，包括通过使用WEC的下丘脑核与喂养行为有关的核。

项目成果

Mouse Shh is required for prechordal plate maintenance during brain and craniofacial morphogenesis

• DOI: 10.1016/j.ydbio.2008.11.022
• 发表时间: 2009-03-01
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Aoto, Kazushi;Shikata, Yayoi;Motoyama, Jun
• 通讯作者: Motoyama, Jun

ヒト正常および腫瘍株化顆粒膜細胞におけるステロイド代謝産物の異なる作用

类固醇代谢物对人类正常和肿瘤来源的颗粒细胞的不同作用

• 发表时间: 2006
• 期刊: 解剖学雑誌 81(supplement)
• 作者: 高橋則行;今井元;伊藤正則;木口一成;石塚文平;塩田清二
• 通讯作者: 塩田清二

Contribution of foregut endoderm to the development of adenohypophysis

前肠内胚层对腺垂体发育的贡献

• 发表时间: 2005
• 期刊: Acta Anatomica Nipponica 80(supplement)
• 作者: Imai H.;Takahashi N.;Suzuki R.;Shioda S.
• 通讯作者: Shioda S.

Contribution of non-neural anterior neural ridge(ANR)of rostral ectoderm-endoderm boundary on the development of adenohypophysis

头端外胚层-内胚层边界非神经性前神经嵴（ANR）对腺垂体发育的贡献

• 发表时间: 2005
• 期刊: Abstracts of the 15th ICCE(Published online in Wiley InterScience)
• 作者: Imai H.;Takahashi N.;Suzuki R.;Shioda S.
• 通讯作者: Shioda S.

The induction and differentiation of the cells contributing to primary oral cavity

初级口腔细胞的诱导和分化

• 发表时间: 2005
• 作者: Imai H.;Takahashi N.;Suzuki R.;Shioda S.;今井元;Imai H
• 通讯作者: Imai H