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The establishment of methods for reproduction of functional teeth by use of long-term culture

建立利用长期培养繁殖功能性牙齿的方法

基本信息

批准号：
15390553
负责人：
IMAI Hajime
金额：
$ 9.28万
依托单位：
Showa University (2004-2006)Tokyo Medical and Dental University (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390553/
关键词：
whole embryo culture (WEC)the long-term maxillofacial organ culture tooth-reproduction anterior axial mesendoderm prechordal plate anterior neural ridge (ANR)Rathke's pouch pituitary mormone 頭部中内胚葉歯腺性下垂体再生法ラット全胚培養法上顔面器官培養法

项目摘要

The purposes 1 and 2 of this study was "making an index about multipotent-rerated genes in cranial neural crest cells (NCCs) by the elucidating gene expression during tooth development" and "establishing a method to produce multipotenial cells by use of the index mentioned above", respectively. However, the amount of RNA of NCCs in the histological section was too small to make the index mentioned above. Therefore, I had stopped the experiments. Therefore, we mainly carried out the studies for "establishing methods reproducing the tooth, periodontal tissues and other craniofacial organs by use of rat whole embryo culture (WEC)", i.e., the purposes 3.During above experimens, we focused on the differences between developmental processes of tooth and pituitary, and carried out the studies. We got new findings as follows. 1. The ectoderm-endoderm boundaries and midbrain-hindbrain NCCs contributes to tooth initiation. 2. The non-neuronal anterior neural ridge (ANR) of ectoderm-endoderm boundaries gives rise to ACTH-producing cells. 3. The prechordal plate (PrP) intrudes itself in ANR and neural plate, contributes to prechordal mesenchyme and intraforebrain mesenchyme, and gives rise to endocrine cells in pituitary. 4. The site-specific distribution of PrP-derived cells is required to the differentiation of the tooth, adenohypophysis and hypothalamus. 5. The many developmental defects occur in PrP-ablation mice similarly to in Shh K.O. mice. We established the niche for reproducing the cells producing pituitary hormones, e.g., ACTH,αGSU,αMsh, TSHβ and LHβ, in the above results 3 by the long-term maxillofacial organ culture of Rathck's pouch accompanied with diencephalon. 6. We show the domain that is important for reproduction of tooth, jaw in a development process of a chin of birds. 7. We are establishing the reproduction methods to craniofacial organ, including hypothalamic nucleus relating to feeding behavior, by use of WEC.

本研究的目的1和目的2分别是“通过阐明牙齿发育过程中基因的表达来建立颅神经脊细胞多能基因的指标”和“建立一种利用上述指标生产多潜能细胞的方法”。然而，组织切片中的NCCs的RNA含量太少，不能作为上述指标。因此，我停止了实验。为此，我们主要开展了“利用大鼠全胚胎培养建立牙齿、牙周组织和其他头面部器官的复制方法”的研究，即目的3。在上述实验中，我们重点研究了牙齿和脑垂体发育过程的差异，并进行了研究。我们的新发现如下。1.外胚层-内胚层边界和中脑-后脑NCCs参与了牙齿的形成。2.外胚层-内胚层边界的非神经性前神经脊(ANR)形成ACTH分泌细胞。3.前脑索间质(PrP)侵入ANR和神经板，形成前索间充质和前脑内间充质，形成内分泌细胞。4.PrP来源细胞的定位分布是牙齿、腺垂体和下丘脑分化所必需的。5.PrP消融小鼠与Shh K.O.小鼠相似，出现许多发育缺陷。在上述结果3中，我们通过长期的颌面部器官培养建立了分泌垂体激素的细胞，如促肾上腺皮质激素、促甲状腺激素β和促黄体生成素β的生态位。6.我们展示了在鸟类下巴发育过程中对牙齿、颌骨的繁殖至关重要的区域。7.我们正在利用WEC建立与摄食行为有关的头面部器官，包括下丘脑核团的复制方法。