Investigation of resin adhesive mechanism to carious dentin and the development of a new remineralization therapy of demineralized dentin

本研究以beagle犬为实验对象，研究树脂粘接剂对龋样脱矿牙本质的体外和体内修复机制，建立一种新的脱矿牙本质再矿化治疗方法和牙本质再生粘接剂修复体系。本研究结果如下（1 ~ 6）；树脂对龋齿牙本质的体外粘接机理研究结果表明，树脂单体对脱矿牙本质的渗透性增加，使其具有优异的粘接性能。结合剂释放的氟化物对脱矿牙本质的再矿化是有效的。实验再矿化液RE-06能促进脱矿牙本质的再矿化，但不影响牙本质的树脂粘接性能。体外实验表明，胶原偶联EVA膜对培养的牙髓细胞具有良好的亲和性。CTGF可促进体外培养牙髓细胞胶原蛋白和骨连接素的生成。狗的体内实验表明，牙本质可以在暴露的牙髓区域保持适当的空间进行再生。这些结果表明可以开发牙本质再矿化和牙本质再生胶粘剂系统。

The aim of this study was to investigate resin adhesive mechanism to caries-like demineralized dentin in vitro and in vivo by using beagle dogs, and to establish a new remineralization therapy of demineralized dentin and dentin - regenerative adhesive restoration system. The results of this study were as follows (1〜6) ;1.The results of the investigation of resin adhesive mechanism to carious dentin in vitro revealed that the increase of the permeability of resin monomer into demineralized dentin resulted in the excellent adhesive property.2.The release of fluoride from bonding agents was effective to the remineralization of demineralized dentin in vitro.3.The experimental re-mineralization solution (RE-06) was shown to promote the remineralization of demineralized dentin without the decrease of resin adhesive property in vitro.4.Collagen - conjugated EVA films were indicated to have an excellent affinity to cultured pulp cells in vitro.5.CTGF was suggested to promote the production of collagen and osteonectin of cultured pulp cells in vitro.6.In vivo dog experiments suggested that dentin could be regenerated on the exposed pulp area by keeping suitable spaces.These results suggested that dentin-remineralizing and dentin-regenerative adhesive systems can be developed.