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Investigation of resin adhesive mechanism to carious dentin and the development of a new remineralization therapy of demineralized dentin

树脂对龋齿牙本质的粘附机制研究及新型脱矿牙本质再矿化疗法的开发

基本信息

批准号：
15390576
负责人：
YOSHIYAMA Masahiro
金额：
$ 4.1万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

The aim of this study was to investigate resin adhesive mechanism to caries-like demineralized dentin in vitro and in vivo by using beagle dogs, and to establish a new remineralization therapy of demineralized dentin and dentin - regenerative adhesive restoration system. The results of this study were as follows (1〜6) ;1.The results of the investigation of resin adhesive mechanism to carious dentin in vitro revealed that the increase of the permeability of resin monomer into demineralized dentin resulted in the excellent adhesive property.2.The release of fluoride from bonding agents was effective to the remineralization of demineralized dentin in vitro.3.The experimental re-mineralization solution (RE-06) was shown to promote the remineralization of demineralized dentin without the decrease of resin adhesive property in vitro.4.Collagen - conjugated EVA films were indicated to have an excellent affinity to cultured pulp cells in vitro.5.CTGF was suggested to promote the production of collagen and osteonectin of cultured pulp cells in vitro.6.In vivo dog experiments suggested that dentin could be regenerated on the exposed pulp area by keeping suitable spaces.These results suggested that dentin-remineralizing and dentin-regenerative adhesive systems can be developed.

本研究旨在通过Beagle犬离体和体内实验，探讨树脂对类龋脱矿牙本质的粘附机制，建立一种新的脱矿牙本质和牙本质再生粘附修复体系的治疗方法。本研究结果如下（1 - 6）; 1.树脂对龋坏牙本质的体外粘接机理研究结果表明，树脂单体对脱矿牙本质的渗透性增加，从而使树脂具有良好的粘接性能; 2.粘接剂中氟离子的释放对脱矿牙本质的体外再矿化有促进作用; 3.实验用的再矿化液（RE-100）对脱矿牙本质的体外再矿化有促进作用，其作用机理为：1.树脂单体对脱矿牙本质的渗透性增加，从而使树脂单体对脱矿牙本质的渗透性增加; 2.粘接剂中氟离子的释放对脱矿牙本质的体外再矿化有促进作用。06）促进脱矿牙本质的再矿化而不降低树脂的粘附性能。结果表明，交联伊娃膜对体外培养的牙髓细胞具有良好的亲和性; 5. CTGF能促进体外培养的牙髓细胞合成胶原和骨粘连蛋白; 6.狗体实验表明，暴露牙髓区可再生牙本质这些结果表明，牙本质再生粘接系统和牙本质再生粘接系统是可以开发的。